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Literature summary extracted from

  • Burrell, M.; Hanfrey, C.C.; Murray, E.J.; Stanley-Wall, N.R.; Michael, A.J.
    Evolution and multiplicity of arginine decarboxylases in polyamine biosynthesis and essential role in Bacillus subtilis biofilm formation (2010), J. Biol. Chem., 285, 39224-39238.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.19 expressed in Escherichia coli BL21(DE3) cells Bacillus subtilis
4.1.1.19 expressed in Escherichia coli BL21(DE3) cells Clostridioides difficile
4.1.1.19 expressed in Escherichia coli BL21(DE3) cells Chloroflexus aurantiacus
4.1.1.19 expressed in Escherichia coli BL21(DE3) cells Gramella forsetii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.19 0.59
-
 L-arginine at pH 6.5, 70°C Chloroflexus aurantiacus
4.1.1.19 0.63
-
 L-arginine at pH 7.5, 37°C Bacillus subtilis
4.1.1.19 0.73
-
 L-arginine at pH 7.5, 60°C Clostridioides difficile
4.1.1.19 1.1
-
 L-arginine at pH 7.5, 70°C Bacillus subtilis
4.1.1.19 2.2
-
 L-arginine at pH 8.5, 30°C Gramella forsetii
4.1.1.19 7.5
-
 L-ornithine at pH 7.5, 70°C Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.19 Bacillus subtilis
-
-
-
4.1.1.19 Bacillus subtilis 168
-
-
-
4.1.1.19 Chloroflexus aurantiacus
-
-
-
4.1.1.19 Chloroflexus aurantiacus J-10-fl
-
-
-
4.1.1.19 Clostridioides difficile
-
-
-
4.1.1.19 Clostridioides difficile 630
-
-
-
4.1.1.19 Gramella forsetii
-
-
-
4.1.1.19 Gramella forsetii KT0803
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.19 HiTrap Chelating HP column chromatography Bacillus subtilis
4.1.1.19 HiTrap Chelating HP column chromatography Clostridioides difficile
4.1.1.19 HiTrap Chelating HP column chromatography Chloroflexus aurantiacus
4.1.1.19 HiTrap Chelating HP column chromatography Gramella forsetii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.19 0.39
-
 at pH 7.5, 37°C Gramella forsetii
4.1.1.19 1.5
-
 at pH 7.5, 37°C Chloroflexus aurantiacus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.19 L-arginine
-
 Bacillus subtilis agmatine + CO2
-
 ?
4.1.1.19 L-arginine
-
 Clostridioides difficile agmatine + CO2
-
 ?
4.1.1.19 L-arginine
-
 Chloroflexus aurantiacus agmatine + CO2
-
 ?
4.1.1.19 L-arginine
-
 Gramella forsetii agmatine + CO2
-
 ?
4.1.1.19 L-arginine
-
 Bacillus subtilis 168 agmatine + CO2
-
 ?
4.1.1.19 L-arginine
-
 Chloroflexus aurantiacus J-10-fl agmatine + CO2
-
 ?
4.1.1.19 L-arginine
-
 Clostridioides difficile 630 agmatine + CO2
-
 ?
4.1.1.19 L-arginine
-
 Gramella forsetii KT0803 agmatine + CO2
-
 ?
4.1.1.19 L-lysine activity is 1.9% of that with L-arginine Chloroflexus aurantiacus 1,5-diaminopentane + CO2
-
 ?
4.1.1.19 L-lysine activity is 1.9% of that with L-arginine Chloroflexus aurantiacus J-10-fl 1,5-diaminopentane + CO2
-
 ?
4.1.1.19 L-ornithine very low activity Bacillus subtilis 1,4-diaminobutane + CO2
-
 ?
4.1.1.19 L-ornithine activity is 1.4% of that with L-arginine Chloroflexus aurantiacus 1,4-diaminobutane + CO2
-
 ?
4.1.1.19 L-ornithine very low activity Bacillus subtilis 168 1,4-diaminobutane + CO2
-
 ?
4.1.1.19 L-ornithine activity is 1.4% of that with L-arginine Chloroflexus aurantiacus J-10-fl 1,4-diaminobutane + CO2
-
 ?
4.1.1.19 meso-diaminopimelate activity is less than 2% of that with L-arginine Chloroflexus aurantiacus ?
-
 ?
4.1.1.19 meso-diaminopimelate activity is less than 2% of that with L-arginine Chloroflexus aurantiacus J-10-fl ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.19 ADC
-
 Bacillus subtilis
4.1.1.19 ADC
-
 Clostridioides difficile
4.1.1.19 ADC
-
 Chloroflexus aurantiacus
4.1.1.19 ADC
-
 Gramella forsetii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.19 30
-
-
 Gramella forsetii
4.1.1.19 75
-
 optimum temperature at above 75°C Bacillus subtilis
4.1.1.19 80
-
 ADC is most active at temperatures above 80°C Chloroflexus aurantiacus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.19 70 80 at 70°C, full enzyme activity is retained, even after 30 min of incubation. Enzyme activity is almost completely abolished within 5 min by incubating the enzyme at 80°C Chloroflexus aurantiacus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.19 0.025
-
 L-arginine at pH 7.5, 60°C Clostridioides difficile
4.1.1.19 0.04
-
 L-ornithine at pH 7.5, 70°C Bacillus subtilis
4.1.1.19 0.21
-
 L-arginine at pH 7.5, 37°C Bacillus subtilis
4.1.1.19 0.48
-
 L-arginine at pH 8.5, 30°C Gramella forsetii
4.1.1.19 1.4
-
 L-arginine at pH 7.5, 70°C Bacillus subtilis
4.1.1.19 11
-
 L-arginine at pH 6.5, 70°C Chloroflexus aurantiacus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.19 6.5
-
-
 Chloroflexus aurantiacus
4.1.1.19 7.7
-
-
 Bacillus subtilis
4.1.1.19 8.5
-
-
 Gramella forsetii

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.19 pyridoxal 5'-phosphate
-
 Bacillus subtilis
4.1.1.19 pyridoxal 5'-phosphate
-
 Clostridioides difficile
4.1.1.19 pyridoxal 5'-phosphate
-
 Chloroflexus aurantiacus
4.1.1.19 pyridoxal 5'-phosphate
-
 Gramella forsetii

General Information

EC Number General Information Comment Organism
4.1.1.19 physiological function the Bacillus subtilis biosynthetic aspartate aminotransferase fold arginine decarboxylase is essential for biofilm formation Bacillus subtilis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.1.19 0.0056
-
 L-ornithine at pH 7.5, 70°C Bacillus subtilis
4.1.1.19 0.035
-
 L-arginine at pH 7.5, 60°C Clostridioides difficile
4.1.1.19 0.22
-
 L-arginine at pH 8.5, 30°C Gramella forsetii
4.1.1.19 0.34
-
 L-arginine at pH 7.5, 37°C Bacillus subtilis
4.1.1.19 1.3
-
 L-arginine at pH 7.5, 70°C Bacillus subtilis
4.1.1.19 18
-
 L-arginine at pH 6.5, 70°C Chloroflexus aurantiacus