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Literature summary extracted from

  • Suzuki, R.; Katayama, T.; Kim, B.J.; Wakagi, T.; Shoun, H.; Ashida, H.; Yamamoto, K.; Fushinobu, S.
    Crystal structures of phosphoketolase: thiamine diphosphate-dependent dehydration mechanism (2010), J. Biol. Chem., 285, 34279-34287.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.22 selenomethionine-labeled enzyme is expressed in Escherichia coli B834 (DE3) cells Bifidobacterium breve

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.2.22 cryocrystallography technique, using 24% (w/v) PEG 6000, 0.1 M bicine buffer, pH 9.0 Bifidobacterium breve

Protein Variants

EC Number Protein Variants Comment Organism
4.1.2.22 E479A inactive Bifidobacterium breve
4.1.2.22 H142A the mutant shows reduced kcat with D-fructose 6-phosphate compared to the wild type enzyme Bifidobacterium breve
4.1.2.22 H142N the mutant shows reduced kcat with D-fructose 6-phosphate compared to the wild type enzyme Bifidobacterium breve
4.1.2.22 H320A inactive Bifidobacterium breve
4.1.2.22 H320N inactive Bifidobacterium breve
4.1.2.22 H548A the mutant shows reduced kcat with D-fructose 6-phosphate compared to the wild type enzyme Bifidobacterium breve
4.1.2.22 H553A inactive Bifidobacterium breve
4.1.2.22 H553N inactive Bifidobacterium breve
4.1.2.22 H64A inactive Bifidobacterium breve
4.1.2.22 H64N inactive Bifidobacterium breve
4.1.2.22 H97A inactive Bifidobacterium breve
4.1.2.22 H97N inactive Bifidobacterium breve
4.1.2.22 K605A inactive Bifidobacterium breve
4.1.2.22 N549A inactive Bifidobacterium breve
4.1.2.22 Q321A the mutant shows reduced kcat with D-fructose 6-phosphate compared to the wild type enzyme Bifidobacterium breve
4.1.2.22 S440A the mutant shows reduced kcat with D-fructose 6-phosphate compared to the wild type enzyme Bifidobacterium breve
4.1.2.22 Y501F the mutant shows reduced kcat with D-fructose 6-phosphate compared to the wild type enzyme Bifidobacterium breve

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.22 0.52
-
 phosphate mutant enzyme H548A, pH and temperature not specified in the publication Bifidobacterium breve
4.1.2.22 0.57
-
 phosphate mutant enzyme S440A, pH and temperature not specified in the publication Bifidobacterium breve
4.1.2.22 1.2
-
 phosphate wild type enzyme, pH and temperature not specified in the publication Bifidobacterium breve
4.1.2.22 1.4
-
 D-fructose 6-phosphate mutant enzyme Y501F, pH and temperature not specified in the publication Bifidobacterium breve
4.1.2.22 1.6
-
 phosphate mutant enzyme Q321A, pH and temperature not specified in the publication Bifidobacterium breve
4.1.2.22 1.7
-
 phosphate mutant enzyme H142A, pH and temperature not specified in the publication Bifidobacterium breve
4.1.2.22 2.3
-
 D-fructose 6-phosphate mutant enzyme H548A, pH and temperature not specified in the publication Bifidobacterium breve
4.1.2.22 2.6
-
 phosphate mutant enzyme H142N, pH and temperature not specified in the publication Bifidobacterium breve
4.1.2.22 4.4
-
 D-fructose 6-phosphate mutant enzyme Q321A, pH and temperature not specified in the publication Bifidobacterium breve
4.1.2.22 7.2
-
 D-fructose 6-phosphate mutant enzyme H142N, pH and temperature not specified in the publication Bifidobacterium breve
4.1.2.22 7.4
-
 D-fructose 6-phosphate mutant enzyme H142A, pH and temperature not specified in the publication Bifidobacterium breve
4.1.2.22 9.7
-
 D-fructose 6-phosphate wild type enzyme, pH and temperature not specified in the publication Bifidobacterium breve
4.1.2.22 25.5
-
 phosphate mutant enzyme Y501F, pH and temperature not specified in the publication Bifidobacterium breve
4.1.2.22 53.5
-
 D-fructose 6-phosphate mutant enzyme S440A, pH and temperature not specified in the publication Bifidobacterium breve

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.22 Mg2+ required Bifidobacterium breve

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.22 D-Fructose 6-phosphate + phosphate Bifidobacterium breve
-
 Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
 ?
4.1.2.22 D-xylulose 5-phosphate + phosphate Bifidobacterium breve
-
 acetyl phosphate + D-glyceraldehyde 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.22 Bifidobacterium breve D6PAH1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.22 D-Fructose 6-phosphate + phosphate
-
 Bifidobacterium breve Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
 ?
4.1.2.22 D-xylulose 5-phosphate + phosphate
-
 Bifidobacterium breve acetyl phosphate + D-glyceraldehyde 3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.22 homodimer x-ray crystallography Bifidobacterium breve

Synonyms

EC Number Synonyms Comment Organism
4.1.2.22 XFPK
-
 Bifidobacterium breve
4.1.2.22 xylulose 5-phosphate/fructose 6-phosphate phosphoketolase
-
 Bifidobacterium breve

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.22 0.2
-
 D-fructose 6-phosphate mutant enzyme H142A, pH and temperature not specified in the publication Bifidobacterium breve
4.1.2.22 0.65
-
 D-fructose 6-phosphate mutant enzyme H142N, pH and temperature not specified in the publication Bifidobacterium breve
4.1.2.22 0.99
-
 D-fructose 6-phosphate mutant enzyme Q321A, pH and temperature not specified in the publication Bifidobacterium breve
4.1.2.22 1.67
-
 D-fructose 6-phosphate mutant enzyme H548A, pH and temperature not specified in the publication Bifidobacterium breve
4.1.2.22 3.28
-
 D-fructose 6-phosphate mutant enzyme Y501F, pH and temperature not specified in the publication Bifidobacterium breve
4.1.2.22 5.83
-
 D-fructose 6-phosphate mutant enzyme S440A, pH and temperature not specified in the publication Bifidobacterium breve
4.1.2.22 25.67
-
 D-fructose 6-phosphate wild type enzyme, pH and temperature not specified in the publication Bifidobacterium breve

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.2.22 thiamine diphosphate dependent on Bifidobacterium breve