BRENDA - Enzyme Database

Metabolism of pentose sugars in the hyperthermophilic archaea Sulfolobus solfataricus and Sulfolobus acidocaldarius

Nunn, C.E.; Johnsen, U.; Schoenheit, P.; Fuhrer, T.; Sauer, U.; Hough, D.W.; Danson, M.J.; J. Biol. Chem. 285, 33701-33709 (2010)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.26
expression in Escherichia coli
Saccharolobus solfataricus
2.3.3.9
expression in Escherichia coli
Saccharolobus solfataricus
4.1.3.1
expression in Escherichia coli
Saccharolobus solfataricus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.26
0.1
-
NADH
pH 7.5, 70°C, recombinant enzyme and native enzyme from xylose-grown cell extract
Saccharolobus solfataricus
1.1.1.26
5
-
glyoxylate
pH 7.5, 70°C, recombinant enzyme and native enzyme from xylose-grown cell extract
Saccharolobus solfataricus
1.1.1.359
0.18
-
D-xylose
pH 8.0, 70°C, native enzyme from xylose-grown cell extract; pH 8.0, 70°C, recombinant enzyme
Saccharolobus solfataricus
1.1.1.359
0.47
-
L-arabinose
pH 8.0, 70°C, native enzyme from xylose-grown cell extract
Saccharolobus solfataricus
1.1.1.359
0.5
-
L-arabinose
pH 8.0, 70°C, recombinant enzyme
Saccharolobus solfataricus
1.2.1.26
0.37
-
glycolaldehyde
pH 6.0, 70°C, native enzyme form xylose-grown cells
Saccharolobus solfataricus
2.3.3.9
0.002
-
glyoxylate
pH 8.0, 70°C, native enzyme from xylose-grown cell extract; pH 8.0, 70°C, recombinant enzyme
Saccharolobus solfataricus
2.3.3.9
0.05
-
acetyl-CoA
pH 8.0, 70°C, native enzyme from xylose-grown cell extract
Saccharolobus solfataricus
2.3.3.9
0.06
-
acetyl-CoA
pH 8.0, 70°C, recombinant enzyme
Saccharolobus solfataricus
4.1.3.1
0.96
-
isocitrate
pH 7.5, 70°C, recombinant enzyme
Saccharolobus solfataricus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.3.9
189000
-
-
Saccharolobus solfataricus
4.1.3.1
190000
-
-
Saccharolobus solfataricus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.2.55
2-dehydro-3-deoxy-D-xylonate
Saccharolobus solfataricus
non-phosphorylative Entner-Doudoroff pathway for catabolism of D-xylose and L-arabinose
pyruvate + glycolaldehyde
-
-
?
4.1.2.55
2-dehydro-3-deoxy-D-xylonate
Saccharolobus solfataricus P2
non-phosphorylative Entner-Doudoroff pathway for catabolism of D-xylose and L-arabinose
pyruvate + glycolaldehyde
-
-
?
4.1.2.55
2-dehydro-3-deoxy-L-arabinonate
Saccharolobus solfataricus
non-phosphorylative Entner-Doudoroff pathway for catabolism of D-xylose and L-arabinose
pyruvate + glycolaldehyde
-
-
?
4.1.2.55
2-dehydro-3-deoxy-L-arabinonate
Saccharolobus solfataricus P2
non-phosphorylative Entner-Doudoroff pathway for catabolism of D-xylose and L-arabinose
pyruvate + glycolaldehyde
-
-
?
4.2.1.39
D-gluconate
Saccharolobus solfataricus
-
2-dehydro-3-deoxy-D-gluconate + H2O
-
-
?
4.2.1.39
D-gluconate
Saccharolobus solfataricus P2
-
2-dehydro-3-deoxy-D-gluconate + H2O
-
-
?
4.2.1.39
additional information
Saccharolobus solfataricus
gluconate dehydratase being active only with C6 metabolites
?
-
-
-
4.2.1.39
additional information
Saccharolobus solfataricus P2
gluconate dehydratase being active only with C6 metabolites
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.26
Saccharolobus solfataricus
Q97U35
-
-
1.1.1.26
Saccharolobus solfataricus P2
Q97U35
-
-
1.1.1.359
Saccharolobus solfataricus
O93715
-
-
1.1.1.359
Saccharolobus solfataricus P2
O93715
-
-
1.2.1.26
Saccharolobus solfataricus
Q97UA1
-
-
1.2.1.26
Saccharolobus solfataricus P2
Q97UA1
-
-
2.3.3.9
Saccharolobus solfataricus
Q97YI7
-
-
2.3.3.9
Saccharolobus solfataricus P2
Q97YI7
-
-
4.1.2.55
Saccharolobus solfataricus
O54288
-
-
4.1.2.55
Saccharolobus solfataricus P2
O54288
-
-
4.1.3.1
Saccharolobus solfataricus
Q97YI8
-
-
4.1.3.1
Saccharolobus solfataricus P2
Q97YI8
-
-
4.2.1.39
Saccharolobus solfataricus
-
-
-
4.2.1.39
Saccharolobus solfataricus P2
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.1.26
-
Saccharolobus solfataricus
2.3.3.9
-
Saccharolobus solfataricus
4.1.3.1
-
Saccharolobus solfataricus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.26
culture condition:xylose-grown cell
-
Saccharolobus solfataricus
-
1.1.1.359
culture condition:xylose-grown cell
-
Saccharolobus solfataricus
-
1.2.1.26
culture condition:xylose-grown cell
-
Saccharolobus solfataricus
-
2.3.3.9
culture condition:xylose-grown cell
-
Saccharolobus solfataricus
-
4.1.3.1
culture condition:xylose-grown cell
-
Saccharolobus solfataricus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.26
glyoxylate + NADH + H+
-
715548
Saccharolobus solfataricus
glycolate + NAD+
-
-
-
?
1.1.1.26
glyoxylate + NADH + H+
-
715548
Saccharolobus solfataricus P2
glycolate + NAD+
-
-
-
?
1.1.1.359
D-xylose + NADP+ + H2O
-
715548
Saccharolobus solfataricus
?
-
-
-
?
1.1.1.359
D-xylose + NADP+ + H2O
-
715548
Saccharolobus solfataricus P2
?
-
-
-
?
1.1.1.359
L-arabinose + NADP+ + H2O
-
715548
Saccharolobus solfataricus
?
-
-
-
?
1.1.1.359
L-arabinose + NADP+ + H2O
-
715548
Saccharolobus solfataricus P2
?
-
-
-
?
1.2.1.26
2,5-dioxopentanoate + NADP+ + H2O
the enzyme is specific for NADP+
715548
Saccharolobus solfataricus
2-oxoglutarate + NADPH + 2 H+
-
-
-
?
1.2.1.26
2,5-dioxopentanoate + NADP+ + H2O
the enzyme is specific for NADP+
715548
Saccharolobus solfataricus P2
2-oxoglutarate + NADPH + 2 H+
-
-
-
?
1.2.1.26
DL-glyceraldehyde + NADP+ + H2O
the enzyme is specific for NADP+
715548
Saccharolobus solfataricus
glycerate + NADPH + H+
-
-
-
?
1.2.1.26
DL-glyceraldehyde + NADP+ + H2O
the enzyme is specific for NADP+
715548
Saccharolobus solfataricus P2
glycerate + NADPH + H+
-
-
-
?
1.2.1.26
glycolaldehyde + NADP+ + H2O
the enzyme is specific for NADP+
715548
Saccharolobus solfataricus
glycolate + NADPH + H+
-
-
-
?
1.2.1.26
glycolaldehyde + NADP+ + H2O
the enzyme is specific for NADP+
715548
Saccharolobus solfataricus P2
glycolate + NADPH + H+
-
-
-
?
2.3.3.9
acetyl-CoA + H2O + glyoxylate
-
715548
Saccharolobus solfataricus
(S)-malate + CoA
-
-
-
?
2.3.3.9
acetyl-CoA + H2O + glyoxylate
-
715548
Saccharolobus solfataricus P2
(S)-malate + CoA
-
-
-
?
4.1.2.55
2-dehydro-3-deoxy-D-xylonate
non-phosphorylative Entner-Doudoroff pathway for catabolism of D-xylose and L-arabinose
715548
Saccharolobus solfataricus
pyruvate + glycolaldehyde
-
-
-
?
4.1.2.55
2-dehydro-3-deoxy-D-xylonate
since the substrate is not commercially available a coupled assay is carried out
715548
Saccharolobus solfataricus
pyruvate + glycolaldehyde
-
-
-
r
4.1.2.55
2-dehydro-3-deoxy-D-xylonate
non-phosphorylative Entner-Doudoroff pathway for catabolism of D-xylose and L-arabinose
715548
Saccharolobus solfataricus P2
pyruvate + glycolaldehyde
-
-
-
?
4.1.2.55
2-dehydro-3-deoxy-D-xylonate
since the substrate is not commercially available a coupled assay is carried out
715548
Saccharolobus solfataricus P2
pyruvate + glycolaldehyde
-
-
-
r
4.1.2.55
2-dehydro-3-deoxy-L-arabinonate
non-phosphorylative Entner-Doudoroff pathway for catabolism of D-xylose and L-arabinose
715548
Saccharolobus solfataricus
pyruvate + glycolaldehyde
-
-
-
?
4.1.2.55
2-dehydro-3-deoxy-L-arabinonate
since the substrate is not commercially available a coupled assay is carried out
715548
Saccharolobus solfataricus
pyruvate + glycolaldehyde
-
-
-
r
4.1.2.55
2-dehydro-3-deoxy-L-arabinonate
non-phosphorylative Entner-Doudoroff pathway for catabolism of D-xylose and L-arabinose
715548
Saccharolobus solfataricus P2
pyruvate + glycolaldehyde
-
-
-
?
4.1.2.55
2-dehydro-3-deoxy-L-arabinonate
since the substrate is not commercially available a coupled assay is carried out
715548
Saccharolobus solfataricus P2
pyruvate + glycolaldehyde
-
-
-
r
4.1.2.55
pyruvate + glycolaldehyde
-
715548
Saccharolobus solfataricus
2-dehydro-3-deoxy-D-xylonate
-
-
-
r
4.1.2.55
pyruvate + glycolaldehyde
-
715548
Saccharolobus solfataricus P2
2-dehydro-3-deoxy-D-xylonate
-
-
-
r
4.1.2.55
pyruvate + glycolaldehyde
-
715548
Saccharolobus solfataricus
2-dehydro-3-deoxy-L-arabinonate
-
-
-
r
4.1.3.1
isocitrate
-
715548
Saccharolobus solfataricus
succinate + glyoxylate
-
-
-
?
4.1.3.1
isocitrate
-
715548
Saccharolobus solfataricus P2
succinate + glyoxylate
-
-
-
?
4.2.1.39
D-gluconate
-
715548
Saccharolobus solfataricus
2-dehydro-3-deoxy-D-gluconate + H2O
-
-
-
?
4.2.1.39
D-gluconate
-
715548
Saccharolobus solfataricus P2
2-dehydro-3-deoxy-D-gluconate + H2O
-
-
-
?
4.2.1.39
additional information
gluconate dehydratase being active only with C6 metabolites
715548
Saccharolobus solfataricus
?
-
-
-
-
4.2.1.39
additional information
gluconate dehydratase being active only with C6 metabolites
715548
Saccharolobus solfataricus P2
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.3.3.9
homodimer
-
Saccharolobus solfataricus
4.1.3.1
homotetramer
-
Saccharolobus solfataricus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.26
70
-
assay at
Saccharolobus solfataricus
1.1.1.359
70
-
assay at
Saccharolobus solfataricus
1.2.1.26
70
-
assay at
Saccharolobus solfataricus
2.3.3.9
70
-
assay at
Saccharolobus solfataricus
4.1.2.55
70
-
assay at
Saccharolobus solfataricus
4.1.3.1
70
-
assay at
Saccharolobus solfataricus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.26
7.5
-
assay at
Saccharolobus solfataricus
1.1.1.359
8
-
assay at
Saccharolobus solfataricus
1.2.1.26
6
-
assay at
Saccharolobus solfataricus
2.3.3.9
8
-
assay at
Saccharolobus solfataricus
4.1.2.55
6
-
assay at
Saccharolobus solfataricus
4.1.3.1
7.5
-
assay at
Saccharolobus solfataricus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.26
NADH
-
Saccharolobus solfataricus
1.1.1.359
NADP+
-
Saccharolobus solfataricus
1.2.1.26
NADP+
the enzyme is specific for NADP+
Saccharolobus solfataricus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.26
expression in Escherichia coli
Saccharolobus solfataricus
2.3.3.9
expression in Escherichia coli
Saccharolobus solfataricus
4.1.3.1
expression in Escherichia coli
Saccharolobus solfataricus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.26
NADH
-
Saccharolobus solfataricus
1.1.1.359
NADP+
-
Saccharolobus solfataricus
1.2.1.26
NADP+
the enzyme is specific for NADP+
Saccharolobus solfataricus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.26
0.1
-
NADH
pH 7.5, 70°C, recombinant enzyme and native enzyme from xylose-grown cell extract
Saccharolobus solfataricus
1.1.1.26
5
-
glyoxylate
pH 7.5, 70°C, recombinant enzyme and native enzyme from xylose-grown cell extract
Saccharolobus solfataricus
1.1.1.359
0.18
-
D-xylose
pH 8.0, 70°C, native enzyme from xylose-grown cell extract; pH 8.0, 70°C, recombinant enzyme
Saccharolobus solfataricus
1.1.1.359
0.47
-
L-arabinose
pH 8.0, 70°C, native enzyme from xylose-grown cell extract
Saccharolobus solfataricus
1.1.1.359
0.5
-
L-arabinose
pH 8.0, 70°C, recombinant enzyme
Saccharolobus solfataricus
1.2.1.26
0.37
-
glycolaldehyde
pH 6.0, 70°C, native enzyme form xylose-grown cells
Saccharolobus solfataricus
2.3.3.9
0.002
-
glyoxylate
pH 8.0, 70°C, native enzyme from xylose-grown cell extract; pH 8.0, 70°C, recombinant enzyme
Saccharolobus solfataricus
2.3.3.9
0.05
-
acetyl-CoA
pH 8.0, 70°C, native enzyme from xylose-grown cell extract
Saccharolobus solfataricus
2.3.3.9
0.06
-
acetyl-CoA
pH 8.0, 70°C, recombinant enzyme
Saccharolobus solfataricus
4.1.3.1
0.96
-
isocitrate
pH 7.5, 70°C, recombinant enzyme
Saccharolobus solfataricus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.3.9
189000
-
-
Saccharolobus solfataricus
4.1.3.1
190000
-
-
Saccharolobus solfataricus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.2.55
2-dehydro-3-deoxy-D-xylonate
Saccharolobus solfataricus
non-phosphorylative Entner-Doudoroff pathway for catabolism of D-xylose and L-arabinose
pyruvate + glycolaldehyde
-
-
?
4.1.2.55
2-dehydro-3-deoxy-D-xylonate
Saccharolobus solfataricus P2
non-phosphorylative Entner-Doudoroff pathway for catabolism of D-xylose and L-arabinose
pyruvate + glycolaldehyde
-
-
?
4.1.2.55
2-dehydro-3-deoxy-L-arabinonate
Saccharolobus solfataricus
non-phosphorylative Entner-Doudoroff pathway for catabolism of D-xylose and L-arabinose
pyruvate + glycolaldehyde
-
-
?
4.1.2.55
2-dehydro-3-deoxy-L-arabinonate
Saccharolobus solfataricus P2
non-phosphorylative Entner-Doudoroff pathway for catabolism of D-xylose and L-arabinose
pyruvate + glycolaldehyde
-
-
?
4.2.1.39
D-gluconate
Saccharolobus solfataricus
-
2-dehydro-3-deoxy-D-gluconate + H2O
-
-
?
4.2.1.39
D-gluconate
Saccharolobus solfataricus P2
-
2-dehydro-3-deoxy-D-gluconate + H2O
-
-
?
4.2.1.39
additional information
Saccharolobus solfataricus
gluconate dehydratase being active only with C6 metabolites
?
-
-
-
4.2.1.39
additional information
Saccharolobus solfataricus P2
gluconate dehydratase being active only with C6 metabolites
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.26
-
Saccharolobus solfataricus
2.3.3.9
-
Saccharolobus solfataricus
4.1.3.1
-
Saccharolobus solfataricus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.26
culture condition:xylose-grown cell
-
Saccharolobus solfataricus
-
1.1.1.359
culture condition:xylose-grown cell
-
Saccharolobus solfataricus
-
1.2.1.26
culture condition:xylose-grown cell
-
Saccharolobus solfataricus
-
2.3.3.9
culture condition:xylose-grown cell
-
Saccharolobus solfataricus
-
4.1.3.1
culture condition:xylose-grown cell
-
Saccharolobus solfataricus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.26
glyoxylate + NADH + H+
-
715548
Saccharolobus solfataricus
glycolate + NAD+
-
-
-
?
1.1.1.26
glyoxylate + NADH + H+
-
715548
Saccharolobus solfataricus P2
glycolate + NAD+
-
-
-
?
1.1.1.359
D-xylose + NADP+ + H2O
-
715548
Saccharolobus solfataricus
?
-
-
-
?
1.1.1.359
D-xylose + NADP+ + H2O
-
715548
Saccharolobus solfataricus P2
?
-
-
-
?
1.1.1.359
L-arabinose + NADP+ + H2O
-
715548
Saccharolobus solfataricus
?
-
-
-
?
1.1.1.359
L-arabinose + NADP+ + H2O
-
715548
Saccharolobus solfataricus P2
?
-
-
-
?
1.2.1.26
2,5-dioxopentanoate + NADP+ + H2O
the enzyme is specific for NADP+
715548
Saccharolobus solfataricus
2-oxoglutarate + NADPH + 2 H+
-
-
-
?
1.2.1.26
2,5-dioxopentanoate + NADP+ + H2O
the enzyme is specific for NADP+
715548
Saccharolobus solfataricus P2
2-oxoglutarate + NADPH + 2 H+
-
-
-
?
1.2.1.26
DL-glyceraldehyde + NADP+ + H2O
the enzyme is specific for NADP+
715548
Saccharolobus solfataricus
glycerate + NADPH + H+
-
-
-
?
1.2.1.26
DL-glyceraldehyde + NADP+ + H2O
the enzyme is specific for NADP+
715548
Saccharolobus solfataricus P2
glycerate + NADPH + H+
-
-
-
?
1.2.1.26
glycolaldehyde + NADP+ + H2O
the enzyme is specific for NADP+
715548
Saccharolobus solfataricus
glycolate + NADPH + H+
-
-
-
?
1.2.1.26
glycolaldehyde + NADP+ + H2O
the enzyme is specific for NADP+
715548
Saccharolobus solfataricus P2
glycolate + NADPH + H+
-
-
-
?
2.3.3.9
acetyl-CoA + H2O + glyoxylate
-
715548
Saccharolobus solfataricus
(S)-malate + CoA
-
-
-
?
2.3.3.9
acetyl-CoA + H2O + glyoxylate
-
715548
Saccharolobus solfataricus P2
(S)-malate + CoA
-
-
-
?
4.1.2.55
2-dehydro-3-deoxy-D-xylonate
non-phosphorylative Entner-Doudoroff pathway for catabolism of D-xylose and L-arabinose
715548
Saccharolobus solfataricus
pyruvate + glycolaldehyde
-
-
-
?
4.1.2.55
2-dehydro-3-deoxy-D-xylonate
since the substrate is not commercially available a coupled assay is carried out
715548
Saccharolobus solfataricus
pyruvate + glycolaldehyde
-
-
-
r
4.1.2.55
2-dehydro-3-deoxy-D-xylonate
non-phosphorylative Entner-Doudoroff pathway for catabolism of D-xylose and L-arabinose
715548
Saccharolobus solfataricus P2
pyruvate + glycolaldehyde
-
-
-
?
4.1.2.55
2-dehydro-3-deoxy-D-xylonate
since the substrate is not commercially available a coupled assay is carried out
715548
Saccharolobus solfataricus P2
pyruvate + glycolaldehyde
-
-
-
r
4.1.2.55
2-dehydro-3-deoxy-L-arabinonate
non-phosphorylative Entner-Doudoroff pathway for catabolism of D-xylose and L-arabinose
715548
Saccharolobus solfataricus
pyruvate + glycolaldehyde
-
-
-
?
4.1.2.55
2-dehydro-3-deoxy-L-arabinonate
since the substrate is not commercially available a coupled assay is carried out
715548
Saccharolobus solfataricus
pyruvate + glycolaldehyde
-
-
-
r
4.1.2.55
2-dehydro-3-deoxy-L-arabinonate
non-phosphorylative Entner-Doudoroff pathway for catabolism of D-xylose and L-arabinose
715548
Saccharolobus solfataricus P2
pyruvate + glycolaldehyde
-
-
-
?
4.1.2.55
2-dehydro-3-deoxy-L-arabinonate
since the substrate is not commercially available a coupled assay is carried out
715548
Saccharolobus solfataricus P2
pyruvate + glycolaldehyde
-
-
-
r
4.1.2.55
pyruvate + glycolaldehyde
-
715548
Saccharolobus solfataricus
2-dehydro-3-deoxy-D-xylonate
-
-
-
r
4.1.2.55
pyruvate + glycolaldehyde
-
715548
Saccharolobus solfataricus P2
2-dehydro-3-deoxy-D-xylonate
-
-
-
r
4.1.2.55
pyruvate + glycolaldehyde
-
715548
Saccharolobus solfataricus
2-dehydro-3-deoxy-L-arabinonate
-
-
-
r
4.1.3.1
isocitrate
-
715548
Saccharolobus solfataricus
succinate + glyoxylate
-
-
-
?
4.1.3.1
isocitrate
-
715548
Saccharolobus solfataricus P2
succinate + glyoxylate
-
-
-
?
4.2.1.39
D-gluconate
-
715548
Saccharolobus solfataricus
2-dehydro-3-deoxy-D-gluconate + H2O
-
-
-
?
4.2.1.39
D-gluconate
-
715548
Saccharolobus solfataricus P2
2-dehydro-3-deoxy-D-gluconate + H2O
-
-
-
?
4.2.1.39
additional information
gluconate dehydratase being active only with C6 metabolites
715548
Saccharolobus solfataricus
?
-
-
-
-
4.2.1.39
additional information
gluconate dehydratase being active only with C6 metabolites
715548
Saccharolobus solfataricus P2
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.3.3.9
homodimer
-
Saccharolobus solfataricus
4.1.3.1
homotetramer
-
Saccharolobus solfataricus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.26
70
-
assay at
Saccharolobus solfataricus
1.1.1.359
70
-
assay at
Saccharolobus solfataricus
1.2.1.26
70
-
assay at
Saccharolobus solfataricus
2.3.3.9
70
-
assay at
Saccharolobus solfataricus
4.1.2.55
70
-
assay at
Saccharolobus solfataricus
4.1.3.1
70
-
assay at
Saccharolobus solfataricus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.26
7.5
-
assay at
Saccharolobus solfataricus
1.1.1.359
8
-
assay at
Saccharolobus solfataricus
1.2.1.26
6
-
assay at
Saccharolobus solfataricus
2.3.3.9
8
-
assay at
Saccharolobus solfataricus
4.1.2.55
6
-
assay at
Saccharolobus solfataricus
4.1.3.1
7.5
-
assay at
Saccharolobus solfataricus
General Information
EC Number
General Information
Commentary
Organism
4.2.1.39
metabolism
the enzyme is involved in the non-phosphorylative version of the Entner-Doudoroff pathway, overview. In vitro kinetic analyses of glucose dehydrogenase, gluconate dehydratase, and KDG-aldolase from Sulfolobus solfataricus show, that these enzymes are also capable of catalyzing the catabolism of galactose, the C4 epimer of glucose, to pyruvate and glyceraldehyde. Gluconate dehydratase is active only with C6 metabolites, thus it is not involved in the C5-specific branch of the non-phosphorylative version of the Entner-Doudoroff pathway
Saccharolobus solfataricus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
4.2.1.39
metabolism
the enzyme is involved in the non-phosphorylative version of the Entner-Doudoroff pathway, overview. In vitro kinetic analyses of glucose dehydrogenase, gluconate dehydratase, and KDG-aldolase from Sulfolobus solfataricus show, that these enzymes are also capable of catalyzing the catabolism of galactose, the C4 epimer of glucose, to pyruvate and glyceraldehyde. Gluconate dehydratase is active only with C6 metabolites, thus it is not involved in the C5-specific branch of the non-phosphorylative version of the Entner-Doudoroff pathway
Saccharolobus solfataricus