EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.14.12 | expression in Escherichia coli | Mycobacterium tuberculosis |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.14.14.12 | hanging drop vapor-diffusion method at 18°C, the crystal structure of HsaA to 2.5 A resolution reveales that the enzyme has the same fold, flavin-binding site, and catalytic residues as p-hydroxyphenyl acetate hydroxylase. HsaA prepared aerobically from the Escherichia coli host exists as a mixture of dimers and octamers in solution while HsaA prepared anaerobically from the rhodococcal host exists as a tetramer | Mycobacterium tuberculosis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.12 | riboflavin | 0.004 mM, 50% inhibition | Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.12 | 0.097 | - |
3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione | flavin: FADH2, pH 7.4, 25°C | Mycobacterium tuberculosis | |
1.14.14.12 | 0.1 | - |
O2 | flavin: FMNH2, pH 7.4, 25°C | Mycobacterium tuberculosis | |
1.14.14.12 | 0.2 | - |
3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione | flavin: FMNH2, pH 7.4, 25°C | Mycobacterium tuberculosis | |
1.14.14.12 | 0.35 | - |
3,17-dihydroxy-9,10-seconandrost-1,3,5(10)-triene-9-one | flavin: FMNH2, pH 7.4, 25°C | Mycobacterium tuberculosis | |
1.14.14.12 | 1.44 | - |
3-hydroxybiphenyl | flavin: FMNH2, pH 7.4, 25°C | Mycobacterium tuberculosis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.12 | 3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione + FADH2 + O2 | Mycobacterium tuberculosis | the enzyme is involved in cholesterol catabolic pathway. This pathway contributes to the pathogenicity of Mycobacterium tuberculosis | 3,4-dihydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione + FAD + H2O | - |
? | |
1.14.14.12 | 3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione + FADH2 + O2 | Mycobacterium tuberculosis H37Rv | the enzyme is involved in cholesterol catabolic pathway. This pathway contributes to the pathogenicity of Mycobacterium tuberculosis | 3,4-dihydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione + FAD + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.12 | Mycobacterium tuberculosis | P9WJA1 | P96852: oxygenase subunit hsaA. The reductase subunit hsaB of the two-component enzyme utilizes NADH to reduce the flavin, which is then transferred to the oxygenase subunit | - |
1.14.14.12 | Mycobacterium tuberculosis H37Rv | P9WJA1 | P96852: oxygenase subunit hsaA. The reductase subunit hsaB of the two-component enzyme utilizes NADH to reduce the flavin, which is then transferred to the oxygenase subunit | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.14.12 | - |
Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.12 | 3,17-dihydroxy-9,10-seconandrost-1,3,5(10)-triene-9-one + FMNH2 + O2 | - |
Mycobacterium tuberculosis | 3,4,17-trihydroxy-9,10-seconandrost-1,3,5(10)-triene-9-one + FMN + H2O | - |
? | |
1.14.14.12 | 3,17-dihydroxy-9,10-seconandrost-1,3,5(10)-triene-9-one + FMNH2 + O2 | - |
Mycobacterium tuberculosis H37Rv | 3,4,17-trihydroxy-9,10-seconandrost-1,3,5(10)-triene-9-one + FMN + H2O | - |
? | |
1.14.14.12 | 3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione + FADH2 + O2 | - |
Mycobacterium tuberculosis | 3,4-dihydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione + FAD + H2O | - |
? | |
1.14.14.12 | 3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione + FADH2 + O2 | the enzyme is involved in cholesterol catabolic pathway. This pathway contributes to the pathogenicity of Mycobacterium tuberculosis | Mycobacterium tuberculosis | 3,4-dihydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione + FAD + H2O | - |
? | |
1.14.14.12 | 3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione + FADH2 + O2 | - |
Mycobacterium tuberculosis H37Rv | 3,4-dihydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione + FAD + H2O | - |
? | |
1.14.14.12 | 3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione + FADH2 + O2 | the enzyme is involved in cholesterol catabolic pathway. This pathway contributes to the pathogenicity of Mycobacterium tuberculosis | Mycobacterium tuberculosis H37Rv | 3,4-dihydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione + FAD + H2O | - |
? | |
1.14.14.12 | 3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione + FMNH2 + O2 | - |
Mycobacterium tuberculosis | 3,4-dihydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione + FMN + H2O | - |
? | |
1.14.14.12 | 3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione + FMNH2 + O2 | - |
Mycobacterium tuberculosis H37Rv | 3,4-dihydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione + FMN + H2O | - |
? | |
1.14.14.12 | 3-hydroxybiphenyl + FMNH2 + O2 | - |
Mycobacterium tuberculosis | 2,3-dihydroxybiphenyl + FMN + H2O | - |
? | |
1.14.14.12 | additional information | the enzyme is able to catalyze the o-hydroxylation of a range of p-substituted phenols to generate the corresponding catechols | Mycobacterium tuberculosis | ? | - |
? | |
1.14.14.12 | additional information | the enzyme is able to catalyze the o-hydroxylation of a range of p-substituted phenols to generate the corresponding catechols | Mycobacterium tuberculosis H37Rv | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.12 | 3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione 4-hydroxylase | the two-component enzyme HsaAB consists of an oxygenase subunit HsaA and a reductase subunit hsaB that utilizes NADH to reduce the flavin, which is then transferred to the oxygenase subunit | Mycobacterium tuberculosis |
1.14.14.12 | HsaAB | the two-component enzyme HsaAB consists of an oxygenase subunit HsaA and a reductase subunit hsaB that utilizes NADH to reduce the flavin, which is then transferred to the oxygenase subunit | Mycobacterium tuberculosis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.14.12 | 25 | - |
assay at | Mycobacterium tuberculosis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.12 | 0.031 | - |
O2 | flavin: FMNH2, pH 7.4, 25°C | Mycobacterium tuberculosis | |
1.14.14.12 | 0.075 | - |
3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione | flavin: FADH2, pH 7.4, 25°C | Mycobacterium tuberculosis | |
1.14.14.12 | 0.11 | - |
3-hydroxybiphenyl | flavin: FMNH2, pH 7.4, 25°C | Mycobacterium tuberculosis | |
1.14.14.12 | 0.2 | - |
3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione | flavin: FMNH2, pH 7.4, 25°C | Mycobacterium tuberculosis | |
1.14.14.12 | 0.24 | - |
3,17-dihydroxy-9,10-seconandrost-1,3,5(10)-triene-9-one | flavin: FMNH2, pH 7.4, 25°C | Mycobacterium tuberculosis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.14.12 | 7 | - |
assay at | Mycobacterium tuberculosis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.12 | FADH2 | the enzyme can use FADH or FMNH2 as flavin cofactor | Mycobacterium tuberculosis | |
1.14.14.12 | FMNH2 | the enzyme can use FADH or FMNH2 as flavin cofactor | Mycobacterium tuberculosis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.14.12 | malfunction | hsaA deletion mutant (mutant with a deletion in the oxygenase subunit) does not grow on cholesterol but grows normally on pyruvate. The hsaA deletion mutant (mutant with a deletion in the oxygenase subunit) accumulates 3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione | Mycobacterium tuberculosis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.12 | 0.08 | - |
3-hydroxybiphenyl | flavin: FMNH2, pH 7.4, 25°C | Mycobacterium tuberculosis | |
1.14.14.12 | 0.32 | - |
O2 | flavin: FMNH2, pH 7.4, 25°C | Mycobacterium tuberculosis | |
1.14.14.12 | 0.7 | - |
3,17-dihydroxy-9,10-seconandrost-1,3,5(10)-triene-9-one | flavin: FMNH2, pH 7.4, 25°C | Mycobacterium tuberculosis | |
1.14.14.12 | 0.77 | - |
3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione | flavin: FADH2, pH 7.4, 25°C | Mycobacterium tuberculosis | |
1.14.14.12 | 1 | - |
3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione | flavin: FMNH2, pH 7.4, 25°C | Mycobacterium tuberculosis |