EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.6.1.56 | D119A | the mutant enzyme has about half of the wild-type activity for 8-oxo-dGTP but shows almost no activity for 2-hydroxy-dATP | Homo sapiens |
3.6.1.56 | D119N | the mutant enzyme has about half of the wild-type activity for 8-oxo-dGTP but shows almost no activity for 2-hydroxy-dATP | Homo sapiens |
3.6.1.56 | N33A | mutation decreases catalytic activity toward 2-hydroxy-dATP to 5% of the wild-type activity. The mutant shows 14% of the wild-type 8-oxo-dGTP activity | Homo sapiens |
3.6.1.56 | N33E | mutation decreases catalytic activity toward 2-hydroxy-dATP to 53% of the wild-type activity. Activity towards 8-oxo-dGTP is totally abolished | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.56 | 2-hydroxy-dATP + H2O | Homo sapiens | human MTH1 hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP and 2-oxo-dATP, to monophosphates, thereby preventing the misincorporation of these oxidized nucleotides during replication | 2-hydroxy-dAMP + diphosphate | - |
? | |
3.6.1.56 | 8-oxo-dGTP + H2O | Homo sapiens | human MTH1 hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP and 2-oxo-dATP, to monophosphates, thereby preventing the misincorporation of these oxidized nucleotides during replication. This enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP | 8-oxo-dGMP + diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.1.56 | Homo sapiens | P36639 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.56 | 2-hydroxy-dATP + H2O | - |
Homo sapiens | 2-hydroxy-dAMP + diphosphate | - |
? | |
3.6.1.56 | 2-hydroxy-dATP + H2O | human MTH1 hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP and 2-oxo-dATP, to monophosphates, thereby preventing the misincorporation of these oxidized nucleotides during replication | Homo sapiens | 2-hydroxy-dAMP + diphosphate | - |
? | |
3.6.1.56 | 8-oxo-dGTP + H2O | human MTH1 hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP and 2-oxo-dATP, to monophosphates, thereby preventing the misincorporation of these oxidized nucleotides during replication. This enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP | Homo sapiens | 8-oxo-dGMP + diphosphate | - |
? | |
3.6.1.56 | 8-oxo-dGTP + H2O | MTH1 catalyzes hydrolysis of 8-oxo-dGTP through nucleophilic substitution of water at the beta-phosphate. The side chains of Asp119 and Asn33 play an essential role in 2-OH-dATP recognition, whereas the indole ring of Trp117 may be important for interacting with the purine bases of substrate nucleotides. Solution structure of MTH1 solved by multidimensional heteronuclear NMR spectroscopy, identification of the substrate interaction pocket. This enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP | Homo sapiens | 8-oxo-dGMP + diphosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.6.1.56 | MTH1 | - |
Homo sapiens |