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Literature summary extracted from

  • Nomura, T.; Takizawa, M.; Aoki, J.; Arai, H.; Inoue, K.; Wakisaka, E.; Yoshizuka, N.; Imokawa, G., Dohmae, N.; Takio, K.; Hattori, M.; Matsuo, N.
    Purification, cDNA cloning, and expression of UDP-Gal:glucosylceramide beta-1,4-galactosyltransferase from rat brain (1998), J. Biol. Chem., 273, 13570-13577.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.274 expression in Sf9 cells Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.274 0.0237
-
 beta-D-glucosyl-(1<->1)-ceramide pH 7.4, 37°C Rattus norvegicus
2.4.1.274 0.0806
-
 UDP-galactose pH 7.4, 37°C Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.274 Ca2+ the enzyme is activated by Mn2+ and to a lesser extent by Mg2+ and Ca2+ Rattus norvegicus
2.4.1.274 Mg2+ the enzyme is activated by Mn2+ and to a lesser extent by Mg2+ and Ca2+ Rattus norvegicus
2.4.1.274 Mn2+ the enzyme is activated by Mn2+ and to a lesser extent by Mg2+ and Ca2+ Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.274 44776
-
 x * 44776, calculated from sequence Rattus norvegicus
2.4.1.274 61000
-
 x * 61000, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.274 UDP-galactose + beta-D-glucosyl-(1<->1)-ceramide Rattus norvegicus
-
 UDP + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.274 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.274 glycoprotein contains about 15% N-linked carbohydrate. The polypeptide contains eight putative glycosylation sites Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.274
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.274 brain high activity Rattus norvegicus
-
2.4.1.274 heart
-
 Rattus norvegicus
-
2.4.1.274 kidney
-
 Rattus norvegicus
-
2.4.1.274 liver low activity Rattus norvegicus
-
2.4.1.274 skeletal muscle
-
 Rattus norvegicus
-
2.4.1.274 spleen low activity Rattus norvegicus
-
2.4.1.274 testis
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.274 UDP-galactose + beta-D-glucosyl-(1<->1)-ceramide
-
 Rattus norvegicus UDP + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.274 ? x * 61000, SDS-PAGE Rattus norvegicus
2.4.1.274 ? x * 44776, calculated from sequence Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.274 LacCer synthase
-
 Rattus norvegicus
2.4.1.274 lactosylceramide synthase
-
 Rattus norvegicus
2.4.1.274 UDP-Gal:glucosylceramide beta-1,4-galactosyltransferase
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.274 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.274 7.2
-
 cacodylate buffer Rattus norvegicus
2.4.1.274 7.4
-
 assay at Rattus norvegicus