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Literature summary extracted from

  • Sampei, G.; Baba, S.; Kanagawa, M.; Yanai, H.; Ishii, T.; Kawai, H.; Fukai, Y.; Ebihara, A.; Nakagawa, N.; Kawai, G.
    Crystal structures of glycinamide ribonucleotide synthetase, PurD, from thermophilic eubacteria (2010), J. Biochem., 148, 429-438.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.13 expression in Escherichia coli Thermus thermophilus
6.3.4.13 expression in Escherichia coli Geobacillus kaustophilus
6.3.4.13 expression in Escherichia coli Aquifex aeolicus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.4.13 native enzyme and in complex with ATP, to 2.4 and 1.8 A resolution, respectively. Comparison of crystal structures of GAR-syn from Thermus thermophilus, Geobacillus kaustophilus and Aquifex aeolicus. The orientations of the B domains are varied among GAR-syns and the molecular dynamics simulation suggests the mobility of the B domain. The B loop in the B domain fixes the position of the beta- and gamma-phosphate groups of the bound ATP Aquifex aeolicus
6.3.4.13 native enzyme and in complex with phosphate, with AMP and phosphate, and with AMP and glycine, to 2.21, 2.20, 1.9 and 2.21 A resolution, respectively. Comparison of crystal structures of GAR-syn from Thermus thermophilus, Geobacillus kaustophilus and Aquifex aeolicus. The orientations of the B domains are varied among GAR-syn's and the molecular dynamics simulation suggests the mobility of the B domain. The B loop in the B domain fixes the position of the beta- and gamma-phosphate groups of the bound ATP Geobacillus kaustophilus
6.3.4.13 selenomethionine derivative, to 2.8 A resolution. Comparison of crystal structures of GAR-syn from Thermus thermophilus, Geobacillus kaustophilus and Aquifex aeolicus. The orientations of the B domains are varied among GAR-syns and the molecular dynamics simulation suggests the mobility of the B domain. The B loop in the B domain fixes the position of the beta- and gamma-phosphate groups of the bound ATP Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.13 45000
-
 gel filtration Thermus thermophilus
6.3.4.13 50000
-
 gel filtration Geobacillus kaustophilus

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.13 Aquifex aeolicus O66949
-
-
6.3.4.13 Geobacillus kaustophilus Q5L3C7
-
-
6.3.4.13 Thermus thermophilus Q5SK40
-
-

Subunits

EC Number Subunits Comment Organism
6.3.4.13 monomer 1 * 45000, SDS-PAGE Thermus thermophilus
6.3.4.13 monomer 1 * 50000, SDS-PAGE Geobacillus kaustophilus