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Literature summary extracted from
- A novel thermostable carboxylesterase from Geobacillus thermodenitrificans: evidence for a new carboxylesterase family (2010), J. Biochem., 148, 299-308.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.1.1 | expressed in Escherichia coli M15[pREP4] cells | Geobacillus thermodenitrificans |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 30000 | - |
x * 30000, SDS-PAGE | Geobacillus thermodenitrificans |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.1 | Geobacillus thermodenitrificans | G3JWZ2 | - |
- |
| 3.1.1.1 | Geobacillus thermodenitrificans CMB-A2 | G3JWZ2 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.1 | Ni-NTA column chromatography, gel filtration | Geobacillus thermodenitrificans |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 2.58 | - |
using 4-nitrophenyl caprylate as substrate, at 50°C and pH 8.0 | Geobacillus thermodenitrificans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.1 | 4-nitrophenyl butyrate + H2O | about 80% activity compared to 4-nitrophenyl caprylate | Geobacillus thermodenitrificans | 4-nitrophenol + butyrate | - |
? |  |
| 3.1.1.1 | 4-nitrophenyl butyrate + H2O | about 80% activity compared to 4-nitrophenyl caprylate | Geobacillus thermodenitrificans CMB-A2 | 4-nitrophenol + butyrate | - |
? | |
| 3.1.1.1 | 4-nitrophenyl caprylate + H2O | maximal activity | Geobacillus thermodenitrificans | 4-nitrophenol + caprylate | - |
? | |
| 3.1.1.1 | 4-nitrophenyl caprylate + H2O | maximal activity | Geobacillus thermodenitrificans CMB-A2 | 4-nitrophenol + caprylate | - |
? | |
| 3.1.1.1 | 4-nitrophenyl decanoate + H2O | 65% activity compared to 4-nitrophenyl caprylate | Geobacillus thermodenitrificans | 4-nitrophenol + decanoate | - |
? | |
| 3.1.1.1 | 4-nitrophenyl decanoate + H2O | 65% activity compared to 4-nitrophenyl caprylate | Geobacillus thermodenitrificans CMB-A2 | 4-nitrophenol + decanoate | - |
? | |
| 3.1.1.1 | 4-nitrophenyl laurate + H2O | 58% activity compared to 4-nitrophenyl caprylate | Geobacillus thermodenitrificans | 4-nitrophenol + laurate | - |
? | |
| 3.1.1.1 | 4-nitrophenyl myristate + H2O | 36% activity compared to 4-nitrophenyl caprylate | Geobacillus thermodenitrificans | 4-nitrophenol + myristate | - |
? | |
| 3.1.1.1 | 4-nitrophenyl palmitate + H2O | 12% activity compared to 4-nitrophenyl caprylate | Geobacillus thermodenitrificans | 4-nitrophenol + palmitate | - |
? | |
| 3.1.1.1 | additional information | activity decreases when the chain length increases from C8 to C16, the enzyme does not show any activity on longer acyl chain triglycerides than tributyrin | Geobacillus thermodenitrificans | ? | - |
? | |
| 3.1.1.1 | additional information | activity decreases when the chain length increases from C8 to C16, the enzyme does not show any activity on longer acyl chain triglycerides than tributyrin | Geobacillus thermodenitrificans CMB-A2 | ? | - |
? | |
| 3.1.1.1 | tributyrin + H2O | - |
Geobacillus thermodenitrificans | ? | - |
? | |
| 3.1.1.1 | tributyrin + H2O | - |
Geobacillus thermodenitrificans CMB-A2 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.1 | ? | x * 30000, SDS-PAGE | Geobacillus thermodenitrificans |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.1 | EstGtA2 | - |
Geobacillus thermodenitrificans |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 50 | - |
- |
Geobacillus thermodenitrificans |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 55 | 75 | the enzyme retains over 90% of its maximal activity at 55 and 60°C. However, a decrease in activity is observed at 65°C (83% left), while only 15% of relative activity is observed at 75°C | Geobacillus thermodenitrificans |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 60 | 80 | the enzyme is stable for 42 h at 60°C, however an important decrease in stability is observed at 70°C, where the enzyme retains 63% of its initial activity, down to 43% at 75°C and further to 75% at 80°C after 10 min | Geobacillus thermodenitrificans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 8 | - |
- |
Geobacillus thermodenitrificans |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 6 | 11 | 62% of relative activity at pH 6.0 and 36% at pH 11.0 | Geobacillus thermodenitrificans |
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Release 2023.1 (January 2023)
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