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Literature summary extracted from

  • Fisch, F.; Fleites, C.M.; Delenne, M.; Baudendistel, N.; Hauer, B.; Turkenburg, J.P.; Hart, S.; Bruce, N.C.; Grogan, G.
    A covalent succinylcysteine-like intermediate in the enzyme-catalyzed transformation of maleate to fumarate by maleate isomerase (2010), J. Am. Chem. Soc., 132, 11455-11457.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.2.1.1 im complex with maleate Nocardia farcinica

Protein Variants

EC Number Protein Variants Comment Organism
5.2.1.1 C194A inactive Nocardia farcinica
5.2.1.1 C194S the mutant displays more than 1000fold reduced turnover compared to the wild type enzyme Nocardia farcinica
5.2.1.1 C76S the mutant displays more than 1000fold reduced turnover compared to the wild type enzyme Nocardia farcinica
5.2.1.1 Y133F the mutant shows decreased turnover and Km, but about wild type catalytic efficiency Nocardia farcinica

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.2.1.1 bromomaleate weak inhibitor Nocardia farcinica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.2.1.1 0.00053
-
 Maleate mutant enzyme Y133F, at pH 7.5, temperature not specified in the publication Nocardia farcinica
5.2.1.1 0.0037
-
 Maleate mutant enzyme C76S, at pH 7.5, temperature not specified in the publication Nocardia farcinica
5.2.1.1 0.0046
-
 Maleate wild type enzyme, at pH 7.5, temperature not specified in the publication Nocardia farcinica
5.2.1.1 0.007
-
 Maleate mutant enzyme C194S, at pH 7.5, temperature not specified in the publication Nocardia farcinica

Organism

EC Number Organism UniProt Comment Textmining
5.2.1.1 Nocardia farcinica Q5YXQ1
-
-
5.2.1.1 Nocardia farcinica IFM 10152 Q5YXQ1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.2.1.1 Maleate
-
 Nocardia farcinica Fumarate
-
 ir
5.2.1.1 Maleate
-
 Nocardia farcinica IFM 10152 Fumarate
-
 ir

Subunits

EC Number Subunits Comment Organism
5.2.1.1 dimer dynamic light scattering Nocardia farcinica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.2.1.1 7.5
-
-
 Nocardia farcinica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.2.1.1 0.00035
-
 Maleate mutant enzyme C194S, at pH 7.5, temperature not specified in the publication Nocardia farcinica
5.2.1.1 0.0019
-
 Maleate mutant enzyme C76S, at pH 7.5, temperature not specified in the publication Nocardia farcinica
5.2.1.1 0.32
-
 Maleate mutant enzyme Y133F, at pH 7.5, temperature not specified in the publication Nocardia farcinica
5.2.1.1 2.8
-
 Maleate wild type enzyme, at pH 7.5, temperature not specified in the publication Nocardia farcinica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2.1.1 7.5
-
-
 Nocardia farcinica

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.2.1.1 0.001
-
 bromomaleate pH 7.5, temperature not specified in the publication Nocardia farcinica

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.2.1.1 0.05
-
 Maleate mutant enzyme C194S, at pH 7.5, temperature not specified in the publication Nocardia farcinica
5.2.1.1 0.513
-
 Maleate mutant enzyme C76S, at pH 7.5, temperature not specified in the publication Nocardia farcinica
5.2.1.1 600
-
 Maleate mutant enzyme Y133F, at pH 7.5, temperature not specified in the publication Nocardia farcinica
5.2.1.1 610
-
 Maleate wild type enzyme, at pH 7.5, temperature not specified in the publication Nocardia farcinica