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Literature summary extracted from

  • Minig, M.; Mazzaferro, L.S.; Erra-Balsells, R.; Petroselli, G.; Breccia, J.D.
    alpha-Rhamnosyl-beta-glucosidase-catalyzed reactions for analysis and biotransformations of plant-based foods (2011), J. Agric. Food Chem., 59, 11238-11243.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.168 food industry potential use of the enzyme for biotechnoligical applications, e.g. in aroma modulation of fermented foods Acremonium sp.

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.168 Ethanol hydrolysis only in the presence of 12% ethanol, no enzyme activity in aqueous medium Acremonium sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.168 Acremonium sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.168 to homogeneity, fractionation with ammonium sulfate and two chromatographic steps: butyl-agarose and QAE-Sephadex Acremonium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.168 2-phenethyl rutinoside + H2O purified aromatic an terpenyl rutinosides are incubated as substrates, no activity in aqueous medium, 3.8% in 12% ethanol Acremonium sp. 2-phenylethanol + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside
-
 ?
3.2.1.168 geranyl rutinoside + H2O purified aromatic an terpenyl rutinosides are incubated as substrates, no activity in aqueous medium, 2.9% in 12% ethanol Acremonium sp. geraniol + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside
-
 ?
3.2.1.168 hesperidin + H2O purified aromatic and terpenyl rutinosides are incubated as substrates, 14.3% relative activity in aqueous medium, 21.7% in 12% ethanol Acremonium sp. hesperetin + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside i.e. hesperetin + rutinose ?
3.2.1.168 additional information hydrolysis not detected in aqueous medium without ethanol Acremonium sp. ?
-
 ?
3.2.1.168 neryl rutinoside + H2O purified aromatic an terpenyl rutinosides are incubated as substrates, no activity in aqueous medium, 2.9% in 12% ethanol Acremonium sp. nerol + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.168 alpha-rhamnosyl-beta-glucosidase
-
 Acremonium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.168 30
-
 assay at Acremonium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.168 5
-
 assay at Acremonium sp.