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Literature summary extracted from

  • Bragatto, I.; Genta, F.A.; Ribeiro, A.F.; Terra, W.R.; Ferreira, C.
    Characterization of a beta-1,3-glucanase active in the alkaline midgut of Spodoptera frugiperda larvae and its relation to beta-glucan-binding proteins (2010), Insect Biochem. Mol. Biol., 40, 861-872.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.39 expression in inactive form in Escherichia coli, expression as active enzyme in Pichia pastoris Spodoptera frugiperda

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.39 40400
-
 x * 40400, calculated Spodoptera frugiperda

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Spodoptera frugiperda A6YQV6 member of glycosyl hysrolase family 16
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.39 glycoprotein sequence has five putative glycosylation sites at residues T33, S77, S324, E188 and E193 Spodoptera frugiperda

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.39 from larval midgut Spodoptera frugiperda

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.39 larva
-
 Spodoptera frugiperda
-
3.2.1.39 midgut mRNA coding for SLam is expressed along the whole midgut tissue Spodoptera frugiperda
-
3.2.1.39 additional information mRNA coding for SLam is expressed along the whole midgut tissue, but not in the Malpighian tubules, fat body and carcass Spodoptera frugiperda
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.39 4480
-
 pH 9.0, 30°C Spodoptera frugiperda

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.39 laminarin + H2O
-
 Spodoptera frugiperda ?
-
 ?
3.2.1.39 additional information Enzyme shows low processivity of 0.4. No substrate: yeast beta-1,3-1,6-glucan or other polysaccharides. enzyme is unable to lyse Saccharomyces cerevisiae cells Spodoptera frugiperda ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.39 ? x * 40400, calculated Spodoptera frugiperda

Synonyms

EC Number Synonyms Comment Organism
3.2.1.39 SLam
-
 Spodoptera frugiperda

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.39 29
-
 Laminarin pH 9.0, 30°C Spodoptera frugiperda

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.39 Spodoptera frugiperda calculated
-
 6.1

Expression

EC Number Organism Comment Expression
3.2.1.39 Spodoptera frugiperda mRNA coding for SLam is expressed along the whole midgut tissue, but not in the Malpighian tubules, fat body and carcass additional information