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Literature summary extracted from

  • Cao, H.; Pauff, J.; Hille, R.
    Substrate orientation and the origin of catalytic power in xanthine oxidoreductase (2011), Indian J. Chem., 50, 355-362.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.1.4 recombinant expression in Escherichia coli Rhodobacter capsulatus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.17.1.4 crystal structure determination of the free enzyme and the enzyme in complex with inhibitor alloxanthine Rhodobacter capsulatus

Protein Variants

EC Number Protein Variants Comment Organism
1.17.1.4 R330M the activity with substrate 2-hydroxy-6-methylpurine is only slightly affected Rhodobacter capsulatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.1.4 alloxanthine a mechanism-based inhibitor, binding structure, overview. Inhibition mechanism involves binding to molybdenum, overview Rhodobacter capsulatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.1.4 Fe2+ in a [Fe4-S4] domain Rhodobacter capsulatus
1.17.1.4 Molybdenum
-
 Rhodobacter capsulatus

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.4 Rhodobacter capsulatus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.17.1.4 xanthine + NAD+ + H2O = urate + NADH + H+ reaction mechanism, The reaction is initiated by proton abstraction from the Mo-OH group by Glu730, the active-site base, followed by nucleophilic attack on the carbon to be hydroxylated, and hydride transfer to the Mo-S double bond. Suitable substrate orientation, overview. Arg310 is involved in stablizing the transition state in the course of nucleophilic attack, overview Rhodobacter capsulatus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.4 additional information xanthine and 2-hydroxy-6-methylpurine are substrates. Substrate binding structures, overview Rhodobacter capsulatus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.1.4 heterotetramer alpha2beta2 Rhodobacter capsulatus

General Information

EC Number General Information Comment Organism
1.17.1.4 additional information active site structure, overview Rhodobacter capsulatus