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Literature summary extracted from
- Expression and characterization of fumarase (FUMR) from Rhizopus oryzae (2011), Fungal Biol., 115, 49-53.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.1.2 | gene fumR, overexpression in Escherichia coli strain BL21 (DE3) | Rhizopus arrhizus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.2 | fumarate | the activity of FUMR catalyzing hydration of fumarate to L-malate is completely inhibited by 2 mM fumaric acid | Rhizopus arrhizus |  |
| 4.2.1.2 | Mg2+ | slight inhibition | Rhizopus arrhizus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.2 | 0.46 | - |
(S)-malate | pH 7.4, 30°C, recombinant enzyme | Rhizopus arrhizus | |
| 4.2.1.2 | 3.07 | - |
fumarate | pH 7.4, 30°C, recombinant enzyme | Rhizopus arrhizus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 4.2.1.2 | mitochondrion | - |
Rhizopus arrhizus | 5739 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.2 | Ca2+ | has a small stimulatory effect | Rhizopus arrhizus | |
| 4.2.1.2 | additional information | no effects of Zn2+, Fe2+, or EDTA on enzyme activity | Rhizopus arrhizus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.2 | (S)-malate | Rhizopus arrhizus | - |
fumarate + H2O | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.2 | Rhizopus arrhizus | P55250 | gene fumR | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.2 | (S)-malate | - |
Rhizopus arrhizus | fumarate + H2O | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.1.2 | fumarase | - |
Rhizopus arrhizus |
| 4.2.1.2 | FUMR | - |
Rhizopus arrhizus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.2 | 30 | - |
- |
Rhizopus arrhizus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.2 | 45 | - |
purified recombinant enzyme, stable below | Rhizopus arrhizus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.2 | 7.2 | - |
- |
Rhizopus arrhizus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.2 | 3 | 9 | purified recombinant enzyme, stable | Rhizopus arrhizus |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 4.2.1.2 | physiological function | fumarase catalyzes the reversible hydration of fumarate to L-malate in Rhizopus oryzae | Rhizopus arrhizus |
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