EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.1.2 | expressed in Escherichia coli BL21(DE3) cells | Bacillus subtilis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.1.1.2 | S161A | the mutant shows increased specific activity compared to the wild type enzyme | Bacillus subtilis |
4.1.1.2 | S161T | the mutant shows reduced specific activity compared to the wild type enzyme | Bacillus subtilis |
4.1.1.2 | T165S | the mutant shows reduced specific activity compared to the wild type enzyme | Bacillus subtilis |
4.1.1.2 | T165V | the mutant shows strongly reduced specific activity compared to the wild type enzyme | Bacillus subtilis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.2 | Mn2+ | the wild type enzyme contains 1.4 Mn2+ ions per monomer | Bacillus subtilis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.1.2 | Bacillus subtilis | O34714 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.1.2 | Ni-NTA column chromatography, gel filtration | Bacillus subtilis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.1.1.2 | 4.5 | - |
mutant enzyme T165V, in 50 mM sodium acetate, pH 4.2, temperature not specified in the publication | Bacillus subtilis |
4.1.1.2 | 22 | - |
mutant enzyme T165S, in 50 mM sodium acetate, pH 4.2, temperature not specified in the publication | Bacillus subtilis |
4.1.1.2 | 29 | - |
mutant enzyme S161T, in 50 mM sodium acetate, pH 4.2, temperature not specified in the publication | Bacillus subtilis |
4.1.1.2 | 40 | - |
wild type enzyme, in 50 mM sodium acetate, pH 4.2, temperature not specified in the publication | Bacillus subtilis |
4.1.1.2 | 48 | - |
mutant enzyme S161A, in 50 mM sodium acetate, pH 4.2, temperature not specified in the publication | Bacillus subtilis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.2 | oxalate + H+ | - |
Bacillus subtilis | formate + CO2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.1.2 | OXDC | - |
Bacillus subtilis |