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Literature summary extracted from

  • Chou, H.; Chang, S.; Lee, G.; Chen, Y.; Yeh, T.; Akoh, C.; Shaw, J.
    Site-directed mutagenesis improves the thermostability of a recombinant Picrophilus torridus trehalose synthase and efficiency for the production of trehalose from sweet potato starch (2010), Food Chem., 119, 1017-1022.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
5.4.99.16 A255P 2.0% activity compared to the wild type enzyme Picrophilus torridus
5.4.99.16 D411P 3.2% activity compared to the wild type enzyme Picrophilus torridus
5.4.99.16 D41P 99.6% activity compared to the wild type enzyme Picrophilus torridus
5.4.99.16 E469P 50% activity compared to the wild type enzyme Picrophilus torridus
5.4.99.16 I121P 21.3% activity compared to the wild type enzyme Picrophilus torridus
5.4.99.16 I385P 5.1% activity compared to the wild type enzyme Picrophilus torridus
5.4.99.16 K332P 96.7% activity compared to the wild type enzyme Picrophilus torridus
5.4.99.16 N503P the mutant shows about 39% higher relative activity than that of the wild type at 65°C for 120 min. The trehalose yield of the mutant is 1.3fold higher than that of the wild type with sweet potato starch as substrate at 50°C for 4 h Picrophilus torridus
5.4.99.16 R523P 90.8% activity compared to the wild type enzyme Picrophilus torridus
5.4.99.16 S439P 20.8% activity compared to the wild type enzyme Picrophilus torridus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.99.16 65000
-
 x * 65000, SDS-PAGE Picrophilus torridus

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.16 Picrophilus torridus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.16 HiTrap Chelating HP column chromatography Picrophilus torridus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4.99.16 0.05
-
 cell-free extract, at pH 6.0 and 45°C for 25 min using maltose as substrate Picrophilus torridus
5.4.99.16 0.25
-
 after 5.3fold purification, at pH 6.0 and 45°C for 25 min using maltose as substrate Picrophilus torridus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.16 maltose
-
 Picrophilus torridus alpha,alpha-trehalose
-
 ?
5.4.99.16 starch
-
 Picrophilus torridus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.4.99.16 ? x * 65000, SDS-PAGE Picrophilus torridus

Synonyms

EC Number Synonyms Comment Organism
5.4.99.16 PTTS
-
 Picrophilus torridus
5.4.99.16 Trehalose synthase
-
 Picrophilus torridus
5.4.99.16 TSase
-
 Picrophilus torridus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.4.99.16 65
-
 the wild type enzyme retains 40% of activity after 120 min at 65°C Picrophilus torridus