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Literature summary extracted from

  • Collu, G.; Unver, N.; Peltenburg-Looman, A.M.G.; van der Heijden, R.; Verpoorte, R.; Memelink, J.
    Geraniol 10-hydroxylase, a cytochrome P450 enzyme involved in terpenoid indole alkaloid biosynthesis (2001), FEBS Lett., 508, 215-220.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.83 cloning from a lambda-ZAPII library, recombinant expression in Saccharomyces cerevisiae strain YPH500 or in enzyme-deficient Catharathus roseus cell line MP183L, complementation Catharanthus roseus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.83 membrane
-
 Catharanthus roseus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.83 56300
-
 x * 56300, SDS-PAGE Catharanthus roseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.83 geraniol + [reduced NADPH-hemoprotein reductase] + O2 Catharanthus roseus
-
 10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.83 Catharanthus roseus
-
 gene CYP76B6
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.83 native enzyme about 12fold from membranes by anion exchange and omega-amino octyl agarose chromatography, followed by hydophobic interaction chromatography to homogeneity Catharanthus roseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.83 geraniol + [reduced NADPH-hemoprotein reductase] + O2
-
 Catharanthus roseus 10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.83 ? x * 56300, SDS-PAGE Catharanthus roseus

Synonyms

EC Number Synonyms Comment Organism
1.14.14.83 CYP76B6
-
 Catharanthus roseus
1.14.14.83 G10H
-
 Catharanthus roseus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.83 cytochrome P450
-
 Catharanthus roseus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.14.83 Catharanthus roseus isoelectric focusing
-
 8.7

Expression

EC Number Organism Comment Expression
1.14.14.83 Catharanthus roseus the stress hormone methyljasmonate strongly induces G10h gene expression coordinately with other terpenoid indole alkaloid biosynthesis genes in Catharanthus roseus cell culture up

General Information

EC Number General Information Comment Organism
1.14.14.83 physiological function geraniol 10-hydroxylase is a cytochrome P450 monooxygenase involved in the biosynthesis of iridoid monoterpenoids and several classes of monoterpenoid alkaloids found in a diverse range of plant species. G10H plays a key regulatory role in terpenoid indole alkaloid biosynthesis Catharanthus roseus