EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.3.23 | araL gene, the fourth cistron of the transcriptional unit araABDLMNPQ-abfA, DNA and amino acid sequence determination and analysis, overexpression of the His-tagged enzyme in Escherichia coli | Bacillus subtilis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.3.23 | G12D | strain IQB849 carrying a single-base substitution C to A introduced in the hairpin region displaying an augment in araL'-'lacZ expression of about 30fold in the presence of inducer, the point mutation increases the free energy of the mRNA secondary structure | Bacillus subtilis |
3.1.3.23 | additional information | a translational fusion of the 5'-end of the araL gene to the lacZ reporter gene from Escherichia coli is constructed and integrated into the Bacillus subtilis chromosome. The construct comprises the araL ribosome-binding site, the initiation codon and a fusion between codon 10 of araL and codon 7 of Escherichia coli lacZ | Bacillus subtilis |
3.1.3.23 | T9K/G12D | the double point mutation, C to A and G to T, introduced a compensatory T in the other part of the stem, thus regenerating the stem-loop structure in strain IQB857 and drastically reducing the expression of araL'-'lacZ | Bacillus subtilis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.23 | 24.96 | - |
D-glucose 6-phosphate | pH 7.0, 37°C | Bacillus subtilis | |
3.1.3.23 | 27.36 | - |
D-arabinose 5-phosphate | pH 7.0, 37°C | Bacillus subtilis | |
3.1.3.23 | 29.14 | - |
D-xylulose 5-phosphate | pH 7.0, 37°C | Bacillus subtilis | |
3.1.3.23 | 34.89 | - |
D-fructose 6-phosphate | pH 7.0, 37°C | Bacillus subtilis | |
3.1.3.23 | 40.74 | - |
D-galactose 1-phosphate | pH 7.0, 37°C | Bacillus subtilis | |
3.1.3.23 | 40.78 | - |
D-fructose 1,6-bisphosphate | pH 7.0, 37°C | Bacillus subtilis | |
3.1.3.23 | 50 | - |
4-nitrophenyl phosphate | pH 7.0, 37°C | Bacillus subtilis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.23 | Mg2+ | absolutely required for catalysis, optimal at 15 mM | Bacillus subtilis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.3.23 | 28900 | - |
x * 28900, about, sequence calculation | Bacillus subtilis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.23 | additional information | Bacillus subtilis | AraL shows low specificity and catalytic activity towards several sugar phosphates, which are metabolic intermediates of the glycolytic and pentose phosphate pathways, substrate specificity, overview | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.23 | Bacillus subtilis | - |
araL gene | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.23 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to over 95% homogeneity | Bacillus subtilis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.23 | 4-nitrophenyl phosphate + H2O | AraL is a phosphatase displaying activity towards the synthetic substrate 4-nitrophenyl phosphate, although there is no evidence that pNPPase activity is physiologically relevant | Bacillus subtilis | 4-nitrophenol + phosphate | - |
? | |
3.1.3.23 | D-arabinose 5-phosphate + H2O | - |
Bacillus subtilis | D-arabinose + phosphate | - |
? | |
3.1.3.23 | D-fructose 1,6-bisphosphate + H2O | - |
Bacillus subtilis | ? | - |
? | |
3.1.3.23 | D-fructose 6-phosphate + H2O | - |
Bacillus subtilis | D-fructose + phosphate | - |
? | |
3.1.3.23 | D-galactose 1-phosphate + H2O | - |
Bacillus subtilis | D-galactose + phosphate | - |
? | |
3.1.3.23 | D-glucose 6-phosphate + H2O | - |
Bacillus subtilis | D-glucose + phosphate | - |
? | |
3.1.3.23 | D-xylulose 5-phosphate + H2O | - |
Bacillus subtilis | D-xylulose + phosphate | - |
? | |
3.1.3.23 | additional information | AraL shows low specificity and catalytic activity towards several sugar phosphates, which are metabolic intermediates of the glycolytic and pentose phosphate pathways, substrate specificity, overview | Bacillus subtilis | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.3.23 | ? | x * 28900, about, sequence calculation | Bacillus subtilis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.23 | AraL | - |
Bacillus subtilis |
3.1.3.23 | More | the enzyme is a member of the ubiquitous haloalkanoate dehalogenase superfamily | Bacillus subtilis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.23 | 65 | - |
recombinant enzyme | Bacillus subtilis |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.23 | 25 | 70 | optimal activity at 37°C, no activity at 25°C, profile, overview | Bacillus subtilis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.23 | 0.012 | - |
4-nitrophenyl phosphate | pH 7.0, 37°C | Bacillus subtilis | |
3.1.3.23 | 1.49 | - |
D-fructose 1,6-bisphosphate | pH 7.0, 37°C | Bacillus subtilis | |
3.1.3.23 | 2.49 | - |
D-glucose 6-phosphate | pH 7.0, 37°C | Bacillus subtilis | |
3.1.3.23 | 2.75 | - |
D-xylulose 5-phosphate | pH 7.0, 37°C | Bacillus subtilis | |
3.1.3.23 | 2.817 | - |
D-fructose 6-phosphate | pH 7.0, 37°C | Bacillus subtilis | |
3.1.3.23 | 2.92 | - |
D-arabinose 5-phosphate | pH 7.0, 37°C | Bacillus subtilis | |
3.1.3.23 | 4.28 | - |
D-galactose 1-phosphate | pH 7.0, 37°C | Bacillus subtilis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.23 | 7 | - |
recombinant enzyme | Bacillus subtilis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.23 | 4 | 9 | although, at pH 8 and pH 9, the enzyme activity is considerably lower than at pH 7.0, the values are higher than at pH 6.0, no activity below pH 4.0, profile, overview | Bacillus subtilis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.1.3.23 | metabolism | context of araL within the arabinose metabolic operon araABDLMNPQ-abfA, as involved in the transport of L-arabinose oligomers. the ability of AraL to catabolize several secondary metabolites, related to detoxification of accidental accumulation of phosphorylated metabolites, requires regulation at the genetic level. Production of AraL is regulated by a structure in the translation initiation region of the mRNA, which most probably blocks access to the ribosome-binding site, preventing protein synthesis, existence of a genetic regulatory mechanism controlling the production of AraL, overview | Bacillus subtilis |
3.1.3.23 | physiological function | putative physiological role of the enzyme AraL in the detoxification of accidental accumulation of phosphorylated metabolites | Bacillus subtilis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.23 | 0.00024 | - |
4-nitrophenyl phosphate | pH 7.0, 37°C | Bacillus subtilis | |
3.1.3.23 | 0.0365 | - |
D-fructose 1,6-bisphosphate | pH 7.0, 37°C | Bacillus subtilis | |
3.1.3.23 | 0.0807 | - |
D-fructose 6-phosphate | pH 7.0, 37°C | Bacillus subtilis | |
3.1.3.23 | 0.0943 | - |
D-xylulose 5-phosphate | pH 7.0, 37°C | Bacillus subtilis | |
3.1.3.23 | 0.0998 | - |
D-glucose 6-phosphate | pH 7.0, 37°C | Bacillus subtilis | |
3.1.3.23 | 0.102 | - |
D-galactose 1-phosphate | pH 7.0, 37°C | Bacillus subtilis | |
3.1.3.23 | 0.106 | - |
D-arabinose 5-phosphate | pH 7.0, 37°C | Bacillus subtilis |