Literature summary extracted from
Liang, W.; Ouyang, S.; Shaw, N.; Joachimiak, A.; Zhang, R.; Liu, Z.J.
Conversion of D-ribulose 5-phosphate to D-xylulose 5-phosphate: new insights from structural and biochemical studies on human RPE (2011), FASEB J., 25, 497-504.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
5.1.3.1 |
expressed in Escherichia coli BL21(DE3) cells |
Homo sapiens |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
5.1.3.1 |
hanging drop vapor diffusion method, using 25% (w/v) PEG 3350, 100 mM Bis-Tris (pH 5.5), and 200 mM NaCl |
Homo sapiens |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
5.1.3.1 |
D175A |
inactive |
Homo sapiens |
5.1.3.1 |
D37A |
the mutation almost abolishes the enzymatic activity |
Homo sapiens |
5.1.3.1 |
H35A |
the mutation almost abolishes the enzymatic activity |
Homo sapiens |
5.1.3.1 |
H70A |
completely insoluble |
Homo sapiens |
5.1.3.1 |
L12A |
the mutation results in more than 50% decrease in the activity |
Homo sapiens |
5.1.3.1 |
M141A |
the mutation results in about 30% decrease in the activity |
Homo sapiens |
5.1.3.1 |
M39A |
the mutation results in about 10% decrease in the activity |
Homo sapiens |
5.1.3.1 |
M72A |
the mutation results in an almost 50% decrease in the activity |
Homo sapiens |
5.1.3.1 |
S10A |
the mutation almost abolishes the enzymatic activity |
Homo sapiens |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
5.1.3.1 |
Fe2+ |
RPE uses Fe2+ for catalysis |
Homo sapiens |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
5.1.3.1 |
Homo sapiens |
Q96AT9 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
5.1.3.1 |
Ni-affinity column chromatography and Superdex G75 gel filtration |
Homo sapiens |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
5.1.3.1 |
brain |
- |
Homo sapiens |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
5.1.3.1 |
D-Ribulose 5-phosphate |
- |
Homo sapiens |
D-Xylulose 5-phosphate |
- |
r |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
5.1.3.1 |
D-ribulose 5-phosphate 3-epimerase |
- |
Homo sapiens |
5.1.3.1 |
RPE |
- |
Homo sapiens |