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Literature summary extracted from

  • Ryu, S.Y.; Hong, G.U.; Kim, D.Y.; Ro, J.Y.
    Enolase 1 and calreticulin regulate the differentiation and functions of mast cells from mouse bone marrow cells (2012), Cell. Signal., 24, 60-70.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.11 co-expression of enolase 1 in bone marrow-derived mast cells with calreticulin Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.11 additional information enolase 1 gene silencing by siRNA leading to reduced the mRNA and protein expressions of surface receptor Fc-RIalpha, surface molecules, such as c-kit, CD40, CD40 ligand and 373 VCAM-1, and also reduced granular tryptase in the culture periods, as well as expressions of enolase 1 and calreticulin. Enolase 1 or calreticulin siRNA transfected-bone marrow-derived mast cells remarkably reduce [Ca2+]i levels compared to wild-type bone marrow-derived mast cells. Both protein siRNA transfected-bone marrow-derived mast cells reduced [Ca2+]i levels more than by individual protein transfection, but does not show additive effect Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.11 additional information enolase 1 is a metalloenzyme with an absolute requirement for divalent metal ions Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.11 47450
-
 x * 47450, sequence calculation and mass spectrometry Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate Mus musculus
-
 phosphoenolpyruvate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.11 Mus musculus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.11 bone marrow cell
-
 Mus musculus
-
4.2.1.11 additional information enolase 1 is a glycolytic enzyme expressed in most tissues Mus musculus
-
4.2.1.11 WEHI-3B cell
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate
-
 Mus musculus phosphoenolpyruvate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.11 ? x * 47450, sequence calculation and mass spectrometry Mus musculus

Synonyms

EC Number Synonyms Comment Organism
4.2.1.11 2-phospho-D-glycerate hydrolyase
-
 Mus musculus
4.2.1.11 enolase 1
-
 Mus musculus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.1.11 Mus musculus sequence calculation
-
 6.37

General Information

EC Number General Information Comment Organism
4.2.1.11 malfunction enolase 1 and calreticulin siRNA reduce the [Ca2+]i levels, amounts of total TNF-alpha, and the release of TNF-alpha and leukotrienes, all of which are increased in the bone marrow-derived mast cells activated with antigen/antibody reaction Mus musculus
4.2.1.11 metabolism enolase 1 is a glycolytic enzyme expressed in most tissues Mus musculus
4.2.1.11 physiological function enolase 1 and calreticulin are important proteins in regulating the differentiation and functions of bone marrow-derived mast cells Mus musculus