BRENDA - Enzyme Database

Enolase 1 and calreticulin regulate the differentiation and functions of mast cells from mouse bone marrow cells

Ryu, S.Y.; Hong, G.U.; Kim, D.Y.; Ro, J.Y.; Cell. Signal. 24, 60-70 (2012)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.11
co-expression of enolase 1 in bone marrow-derived mast cells with calreticulin
Mus musculus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
4.2.1.11
additional information
enolase 1 gene silencing by siRNA leading to reduced the mRNA and protein expressions of surface receptor Fc-RIalpha, surface molecules, such as c-kit, CD40, CD40 ligand and 373 VCAM-1, and also reduced granular tryptase in the culture periods, as well as expressions of enolase 1 and calreticulin. Enolase 1 or calreticulin siRNA transfected-bone marrow-derived mast cells remarkably reduce [Ca2+]i levels compared to wild-type bone marrow-derived mast cells. Both protein siRNA transfected-bone marrow-derived mast cells reduced [Ca2+]i levels more than by individual protein transfection, but does not show additive effect
Mus musculus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
additional information
enolase 1 is a metalloenzyme with an absolute requirement for divalent metal ions
Mus musculus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
47450
-
x * 47450, sequence calculation and mass spectrometry
Mus musculus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Mus musculus
-
phosphoenolpyruvate + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Mus musculus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
bone marrow cell
-
Mus musculus
-
4.2.1.11
additional information
enolase 1 is a glycolytic enzyme expressed in most tissues
Mus musculus
-
4.2.1.11
WEHI-3B cell
-
Mus musculus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
714692
Mus musculus
phosphoenolpyruvate + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
?
x * 47450, sequence calculation and mass spectrometry
Mus musculus
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
4.2.1.11
Mus musculus
sequence calculation
-
6.37
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
co-expression of enolase 1 in bone marrow-derived mast cells with calreticulin
Mus musculus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
4.2.1.11
additional information
enolase 1 gene silencing by siRNA leading to reduced the mRNA and protein expressions of surface receptor Fc-RIalpha, surface molecules, such as c-kit, CD40, CD40 ligand and 373 VCAM-1, and also reduced granular tryptase in the culture periods, as well as expressions of enolase 1 and calreticulin. Enolase 1 or calreticulin siRNA transfected-bone marrow-derived mast cells remarkably reduce [Ca2+]i levels compared to wild-type bone marrow-derived mast cells. Both protein siRNA transfected-bone marrow-derived mast cells reduced [Ca2+]i levels more than by individual protein transfection, but does not show additive effect
Mus musculus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
additional information
enolase 1 is a metalloenzyme with an absolute requirement for divalent metal ions
Mus musculus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
47450
-
x * 47450, sequence calculation and mass spectrometry
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Mus musculus
-
phosphoenolpyruvate + H2O
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
bone marrow cell
-
Mus musculus
-
4.2.1.11
additional information
enolase 1 is a glycolytic enzyme expressed in most tissues
Mus musculus
-
4.2.1.11
WEHI-3B cell
-
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
714692
Mus musculus
phosphoenolpyruvate + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
?
x * 47450, sequence calculation and mass spectrometry
Mus musculus
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
4.2.1.11
Mus musculus
sequence calculation
-
6.37
General Information
EC Number
General Information
Commentary
Organism
4.2.1.11
malfunction
enolase 1 and calreticulin siRNA reduce the [Ca2+]i levels, amounts of total TNF-alpha, and the release of TNF-alpha and leukotrienes, all of which are increased in the bone marrow-derived mast cells activated with antigen/antibody reaction
Mus musculus
4.2.1.11
metabolism
enolase 1 is a glycolytic enzyme expressed in most tissues
Mus musculus
4.2.1.11
physiological function
enolase 1 and calreticulin are important proteins in regulating the differentiation and functions of bone marrow-derived mast cells
Mus musculus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
4.2.1.11
malfunction
enolase 1 and calreticulin siRNA reduce the [Ca2+]i levels, amounts of total TNF-alpha, and the release of TNF-alpha and leukotrienes, all of which are increased in the bone marrow-derived mast cells activated with antigen/antibody reaction
Mus musculus
4.2.1.11
metabolism
enolase 1 is a glycolytic enzyme expressed in most tissues
Mus musculus
4.2.1.11
physiological function
enolase 1 and calreticulin are important proteins in regulating the differentiation and functions of bone marrow-derived mast cells
Mus musculus