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Literature summary extracted from

  • Chandrasekharaiah, M.; Thulasi, A.; Bagath, M.; Kumar, D.P.; Santosh, S.S.; Palanivel, C.; Jose, V.L.; Sampath, K.T.
    Molecular cloning, expression and characterization of a novel feruloyl esterase enzyme from the symbionts of termite (Coptotermes formosanus) gut (2011), BMB Rep., 44, 52-57.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.73 gene Ft3-7, DNA and amino acid sequence determination and analysis, expression of His-tagged feruoyl esterase in Escherichia coli strain BL21 uncultured bacterium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.73 additional information uncultured bacterium ability of the purified Ft3-7 protein to release phenolic compounds from crude plant cell wall materials in the form of crop, e.g. wheat bran and spent grain, release of ferulic acid and diferulic acid together with xylanase ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 uncultured bacterium
-
 gene Ft3-7 amplified from a symbiont in the gut of Coptotermes formosanus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.73 recombinant His-tagged feruoyl esterase from Escherichia coli strain BL21 by nickel affinity chromatography uncultured bacterium

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.73 gut
-
 uncultured bacterium
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.73 24.6
-
 recombinant enzyme, with substrate ethyl ferulate, pH 7.0, 37°C uncultured bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 ethyl ferulate + H2O
-
 uncultured bacterium ethanol + ferulate
-
 ?
3.1.1.73 additional information ability of the purified Ft3-7 protein to release phenolic compounds from crude plant cell wall materials in the form of crop, e.g. wheat bran and spent grain, release of ferulic acid and diferulic acid together with xylanase uncultured bacterium ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 FAE
-
 uncultured bacterium
3.1.1.73 More the enzyme belongs to the type-D sub-class of ferulic acid esterases uncultured bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 37
-
 recombinant enzyme uncultured bacterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.73 50
-
 the enzyme is stable below 45°C, it quickly loses activity above 50°C uncultured bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 7
-
 recombinant enzyme uncultured bacterium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.73 4.5 6.9 the enzyme shows a broad optimum uncultured bacterium