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Literature summary extracted from

  • Jonnadula, R.; Ghadi, S.
    Purification and characterization of beta-agarase from seaweed decomposing bacterium Microbulbifer sp. strain CMC-5 (2011), Biotechnol. Bioprocess Eng., 16, 513-519.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.81 NaCl 145% activity at 500 mM NaCl Microbulbifer sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.B1 Ba2+ 85% residual activity at 1 mM Microbulbifer sp.
3.2.1.B1 Co2+ complete inhibition at 1 mM Microbulbifer sp.
3.2.1.B1 Cu2+ complete inhibition at 1 mM Microbulbifer sp.
3.2.1.B1 EDTA 46% residual activity at 1 mM Microbulbifer sp.
3.2.1.B1 Fe2+ complete inhibition at 1 mM Microbulbifer sp.
3.2.1.B1 Hg2+ complete inhibition at 1 mM Microbulbifer sp.
3.2.1.B1 Na+ 60% residual activity at 1 mM, 72% residual activity at 10 mM, 91% residual activity at 100 mM, 83% residual activity at 1 M, complete inhibition at 3 M Microbulbifer sp.
3.2.1.B1 Zn2+ complete inhibition at 1 mM Microbulbifer sp.
3.2.1.81 Ba2+ 85% residual activity at 1 mM Microbulbifer sp.
3.2.1.81 Co2+ complete inhibition at 1 mM Microbulbifer sp.
3.2.1.81 Cu2+ complete inhibition at 1 mM Microbulbifer sp.
3.2.1.81 EDTA 46% residual activity at 1 mM Microbulbifer sp.
3.2.1.81 Fe2+ complete inhibition at 1 mM Microbulbifer sp.
3.2.1.81 Hg2+ complete inhibition at 1 mM Microbulbifer sp.
3.2.1.81 N-bromosuccinimide complete inhibition at 0.1 mM Microbulbifer sp.
3.2.1.81 NaCl 60% residual activity at 1 mM, 72% residual activity at 10 mM, 91% residual activity at 100 mM, 83% residual activity at 1 M, complete inhibition at 3 M Microbulbifer sp.
3.2.1.81 SDS complete inhibition at 1% (w/v) Microbulbifer sp.
3.2.1.81 Zn2+ complete inhibition at 1 mM Microbulbifer sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.B1 extracellular
-
 Microbulbifer sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.B1 Ca2+ 123% activity at 500 mM Microbulbifer sp.
3.2.1.B1 Mg2+ 112% activity at 500 mM Microbulbifer sp.
3.2.1.B1 Na+ 145% activity at 500 mM Microbulbifer sp.
3.2.1.81 Ca2+ 123% activity at 1 mM Microbulbifer sp.
3.2.1.81 Mg2+ 112% activity at 1 mM Microbulbifer sp.
3.2.1.81 Mn2+ 105% activity at 1 mM Microbulbifer sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.B1 59000
-
 x * 59000, SDS-PAGE Microbulbifer sp.
3.2.1.81 59000
-
 x * 59000, SDS-PAGE Microbulbifer sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.B1 Microbulbifer sp.
-
-
-
3.2.1.B1 Microbulbifer sp. CMC-5 (MTCC 9889)
-
-
-
3.2.1.81 Microbulbifer sp.
-
-
-
3.2.1.81 Microbulbifer sp. CMC-5 (MTCC 9889)
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.B1 ultrafiltration, DEAE-Sepharose column chromatography, and Sephacryl S-300HR gel filtration Microbulbifer sp.
3.2.1.81 ultrafiltration, DEAE Sepharose column chromatography and Sephacryl S-300HR gel filtration Microbulbifer sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.B1 0.04
-
 culture supernatant, at pH 7.0 and 30°C Microbulbifer sp.
3.2.1.B1 4.22
-
 after 103.5fold purification, at pH 7.0 and 30°C Microbulbifer sp.
3.2.1.81 0.04
-
 culture supernatant, pH 7.0, 30°C Microbulbifer sp.
3.2.1.81 4.22
-
 after 103.5fold purification, pH 7.0, 30°C Microbulbifer sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.B1 agarose + H2O the purified enzyme is an endo-type beta-agarase able to hydrolyze the beta-1,4 glycosidic linkages of agarose, releasing neoagarotetraose and neoagarohexaose Microbulbifer sp. neoagarotetraose + neoagarohexaose major end products ?
3.2.1.B1 alginate + H2O
-
 Microbulbifer sp. ?
-
 ?
3.2.1.B1 carboxymethyl cellulose + H2O
-
 Microbulbifer sp. ?
-
 ?
3.2.1.B1 carrageenan + H2O
-
 Microbulbifer sp. ?
-
 ?
3.2.1.B1 chitin + H2O
-
 Microbulbifer sp. ?
-
 ?
3.2.1.B1 xylan + H2O
-
 Microbulbifer sp. ?
-
 ?
3.2.1.81 agar + H2O
-
 Microbulbifer sp. ?
-
 ?
3.2.1.81 agar + H2O
-
 Microbulbifer sp. CMC-5 (MTCC 9889) ?
-
 ?
3.2.1.81 agarose + H2O
-
 Microbulbifer sp. neoagarotetraose + neoagarohexaose neoagarotetraose and neoagarohexaose are the major products, neoagarooctaose is also observed as a minor end product ?
3.2.1.81 agarose + H2O
-
 Microbulbifer sp. CMC-5 (MTCC 9889) neoagarotetraose + neoagarohexaose neoagarotetraose and neoagarohexaose are the major products, neoagarooctaose is also observed as a minor end product ?
3.2.1.81 alginate + H2O
-
 Microbulbifer sp. ?
-
 ?
3.2.1.81 carboxymethyl cellulose + H2O
-
 Microbulbifer sp. ?
-
 ?
3.2.1.81 carboxymethyl cellulose + H2O
-
 Microbulbifer sp. CMC-5 (MTCC 9889) ?
-
 ?
3.2.1.81 carrageenan + H2O
-
 Microbulbifer sp. ?
-
 ?
3.2.1.81 chitin + H2O
-
 Microbulbifer sp. ?
-
 ?
3.2.1.81 chitin + H2O
-
 Microbulbifer sp. CMC-5 (MTCC 9889) ?
-
 ?
3.2.1.81 xylan + H2O
-
 Microbulbifer sp. ?
-
 ?
3.2.1.81 xylan + H2O
-
 Microbulbifer sp. CMC-5 (MTCC 9889) ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.B1 ? x * 59000, SDS-PAGE Microbulbifer sp.
3.2.1.81 ? x * 59000, SDS-PAGE Microbulbifer sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.81 endo-type beta-agarase
-
 Microbulbifer sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.B1 50
-
-
 Microbulbifer sp.
3.2.1.81 50
-
 96% of the agarase activity is still observed at 60°C Microbulbifer sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.B1 40 60
-
 Microbulbifer sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.B1 50 70 the agarase retains 62% of its activity after incubating at 50°C for 30 min. Further, 45% of the agarase activity is still retained at 60°C. The agarase activity is completely abolished at 70°C Microbulbifer sp.
3.2.1.81 50 60 the agarase is thermally stable up to 50°C, with 62% of its residual activity is retained and 45% of the agarase activity is still retained at 60°C. The agarase activity is completely abolished at 70°C Microbulbifer sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.B1 7
-
-
 Microbulbifer sp.
3.2.1.81 7
-
-
 Microbulbifer sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.B1 6 8
-
 Microbulbifer sp.
3.2.1.81 6 8 the agarase retains more than 70% of its original activity at pH 6.0 and 8.0 Microbulbifer sp.