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Literature summary extracted from

  • Nishizawa, M.; Yabusaki, Y.; Kanaoka, M.
    Identification of the catalytic residues of carboxylesterase from Arthrobacter globiformis by diisopropyl fluorophosphate-labeling and site-directed mutagenesis (2011), Biosci. Biotechnol. Biochem., 75, 89-94.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.1 mutant enzymes are expressed in Escherichia coli JM105 cells Arthrobacter globiformis

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.1 C60A the mutant shows higher affinity to the substrate (+)-trans-ethyl crysanthemate compared to the wild type enzyme Arthrobacter globiformis
3.1.1.1 K62E inactive with (+)-trans-ethyl crysanthemate Arthrobacter globiformis
3.1.1.1 K62R the mutant shows 7% turnover rate with the substrate (+)-trans-ethyl crysanthemate compared to the wild type enzyme Arthrobacter globiformis
3.1.1.1 KK62L inactive with (+)-trans-ethyl crysanthemate Arthrobacter globiformis
3.1.1.1 S53A the mutant has about wild type affinity to the substrate (+)-trans-ethyl crysanthemate Arthrobacter globiformis
3.1.1.1 S59A inactive with (+)-trans-ethyl crysanthemate Arthrobacter globiformis
3.1.1.1 S61A the mutant shows higher affinity to the substrate (+)-trans-ethyl crysanthemate compared to the wild type enzyme Arthrobacter globiformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.1 0.1
-
 (+)-trans-ethyl crysanthemate mutant enzyme S61A, pH 7.5, 45°C Arthrobacter globiformis
3.1.1.1 0.18
-
 (+)-trans-ethyl crysanthemate mutant enzyme C60A, pH 7.5, 45°C Arthrobacter globiformis
3.1.1.1 0.31
-
 (+)-trans-ethyl crysanthemate mutant enzyme S53A, pH 7.5, 45°C Arthrobacter globiformis
3.1.1.1 0.33
-
 (+)-trans-ethyl crysanthemate wild type enzyme, pH 7.5, 45°C Arthrobacter globiformis
3.1.1.1 10
-
 (+)-trans-ethyl crysanthemate mutant enzyme K62R, pH 7.5, 45°C Arthrobacter globiformis

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Arthrobacter globiformis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1 DEAE-5PW column chromatography Arthrobacter globiformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 (+)-trans 4-nitrophenyl crysanthemate + H2O
-
 Arthrobacter globiformis (+)-trans-crysanthemic acid + 4-nitrophenol
-
 ?
3.1.1.1 (+)-trans-ethyl crysanthemate + H2O
-
 Arthrobacter globiformis (+)-trans-crysanthemic acid + ethanol
-
 ?
3.1.1.1 phenolphthalein dibutyrate + H2O
-
 Arthrobacter globiformis ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 10
-
 wild type enzyme Arthrobacter globiformis