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Literature summary extracted from

  • Kobayashi, M.; Hondoh, H.; Mori, H.; Saburi, W.; Okuyama, M.; Kimura, A.
    Calcium ion-dependent increase in thermostability of dextran glucosidase from Streptococcus mutans (2011), Biosci. Biotechnol. Biochem., 75, 1557-1563.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.70 expressed in Escherichia coli BL21(DE3) CodonPlus RIL cells Streptococcus mutans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.70 Ca2+ the addition of Ca2+ (optimum 5 mM) enhances the thermostability of dextran glucosidase but has no significant effect on catalytic reaction Streptococcus mutans
3.2.1.70 Mg2+ the addition of Mg2+ (optimum 15 mM) enhances the thermostability of dextran glucosidase Streptococcus mutans
3.2.1.70 additional information K+ and Na+ do not enhance the thermostability of dextran glucosidase Streptococcus mutans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.70 dextran + H2O Streptococcus mutans
-
 beta-D-glucose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.70 Streptococcus mutans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.70 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Streptococcus mutans 4-nitrophenol + alpha-D-glucopyranose
-
 ?
3.2.1.70 dextran + H2O
-
 Streptococcus mutans beta-D-glucose + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.70 dextran glucosidase
-
 Streptococcus mutans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.70 46
-
 the enzyme activity drops to 50% in 5 min at 46°C at 0-1 mM Ca2+, but the presence of 2 mM Ca2+ prolongs this to 30 min and 3-30 mM Ca2+ stabilizes the enzyme so that it retains 70% activity or higher even after 60 min incubation at 46°C Streptococcus mutans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.70 5.5 7 the enzyme is stable between pH 5.5 and 7.0 in the presence of 5 mM Ca2+ after 15 min at 37°C Streptococcus mutans
3.2.1.70 5.8 6.7 the enzyme is stable between pH 5.8 and 6.7 in the absence of Ca2+ after 15 min at 37°C Streptococcus mutans