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Literature summary extracted from

  • Park, J.I.; Kent, M.S.; Datta, S.; Holmes, B.M.; Huang, Z.; Simmons, B.A.; Sale, K.L.; Sapra, R.
    Enzymatic hydrolysis of cellulose by the cellobiohydrolase domain of CelB from the hyperthermophilic bacterium Caldicellulosiruptor saccharolyticus (2011), Biores. Technol., 102, 5988-5994.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.91 additional information enzyme domain GH5 activity on avicel and corn stover is enhanced upon pretreatment with 1-ethyl-3-methylimidazolium acetate, and activity is further improved when the CBM3 domain was fused to GH5 Caldicellulosiruptor saccharolyticus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.91 expressed in Escherichia coli Caldicellulosiruptor saccharolyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.91 cellobiose product inhibition by cellobiose Caldicellulosiruptor saccharolyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.91 2.2
-
 4-nitrophenyl beta-D-cellobioside domain GH5, at pH 5.5 and 80°C, Caldicellulosiruptor saccharolyticus
3.2.1.91 2.4
-
 4-nitrophenyl beta-D-cellobioside domain construct CBM3-GH5, at pH 5.5 and 80°C, Caldicellulosiruptor saccharolyticus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.91 Caldicellulosiruptor saccharolyticus A4XIF7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.91 Ni2+-NTA column chromatography and HiTrap Q HP anion-exchange column chromatography Caldicellulosiruptor saccharolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.91 4-nitrophenyl beta-D-cellobioside + H2O
-
 Caldicellulosiruptor saccharolyticus 4-nitrophenol + cellobiose
-
 ?
3.2.1.91 avicel + H2O
-
 Caldicellulosiruptor saccharolyticus cellobiose + ?
-
 ?
3.2.1.91 carboxymethyl cellulose + H2O
-
 Caldicellulosiruptor saccharolyticus ?
-
 ?
3.2.1.91 cellulose + H2O
-
 Caldicellulosiruptor saccharolyticus cellobiose + ?
-
 ?
3.2.1.91 corn stover + H2O
-
 Caldicellulosiruptor saccharolyticus cellobiose + ?
-
 ?
3.2.1.91 additional information 4-nitrophenyl beta-D-glucopyranoside is not a substrate Caldicellulosiruptor saccharolyticus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.91 CBM3-GH5 recombinant enzyme domain construct Caldicellulosiruptor saccharolyticus
3.2.1.91 CelB
-
 Caldicellulosiruptor saccharolyticus
3.2.1.91 GH5 the GH5 domain is a cellobiohydrolase that lacks beta-glucosidase activity Caldicellulosiruptor saccharolyticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.91 80
-
 temperature optimum for domain construct CBM3-GH5 Caldicellulosiruptor saccharolyticus
3.2.1.91 80 90 temperature optimum for domain GH5 Caldicellulosiruptor saccharolyticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.91 70 80 the recombinant GH5 and CBM3-GH5 constructs are both stable at 80°C with half-lives of 23 h and 39 h, respectively, and retain more than 94% activity after 48 h at 70°C. The melting temperature of domain GH5 is at 89°C, while the melting temperature of the domain construct CBM3-GH5 is at 80°C Caldicellulosiruptor saccharolyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.91 4.7
-
 pH-optimum for domain construct CBM3-GH5 Caldicellulosiruptor saccharolyticus
3.2.1.91 5.5
-
 pH-optimum for domain GH5 Caldicellulosiruptor saccharolyticus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.2.1.91 30
-
 domain GH5, in 50 mM MES buffer at pH 5.5 and 80°C Caldicellulosiruptor saccharolyticus cellobiose