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Literature summary extracted from

  • Martel, C.M.; Warrilow, A.G.; Jackson, C.J.; Mullins, J.G.; Togawa, R.C.; Parker, J.E.; Morris, M.S.; Donnison, I.S.; Kelly, D.E.; Kelly, S.L.
    Expression, purification and use of the soluble domain of Lactobacillus paracasei beta-fructosidase to optimise production of bioethanol from grass fructans (2010), Biores. Technol., 101, 4395-4402.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.26 overexpression as truncated cytosolic protein in Escherichia coli Lacticaseibacillus paracasei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.26 0.5
-
 levan pH 5.0, 37°C Lacticaseibacillus paracasei
3.2.1.26 15
-
 nystose pH 5.0, 37°C Lacticaseibacillus paracasei
3.2.1.26 15.6
-
 inulin pH 5.0, 37°C Lacticaseibacillus paracasei
3.2.1.26 49
-
 1-kestose pH 5.0, 37°C Lacticaseibacillus paracasei
3.2.1.26 392
-
 raffinose pH 5.0, 37°C Lacticaseibacillus paracasei
3.2.1.26 398
-
 sucrose pH 5.0, 37°C Lacticaseibacillus paracasei

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.26 Lacticaseibacillus paracasei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.26 recombinant truncated cytosolic protein Lacticaseibacillus paracasei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.26 1-kestose + H2O
-
 Lacticaseibacillus paracasei D-fructose + ?
-
 ?
3.2.1.26 grass juice + H2O hydrolysis of polyfructose moieties in agriculturally-sourced grass juice with recombinant truncated cytosolic enzyme results in the release of more than 13 mg/ml more bioavailable fructose than is measured in untreated grass juice. Bioethanol yields from fermentation experiments with Brewer's yeast and grass juice plus enzymeaere more than 25% higher than those achieved using untreated grass juice feedstock Lacticaseibacillus paracasei ?
-
 ?
3.2.1.26 inulin + H2O
-
 Lacticaseibacillus paracasei D-fructose + ?
-
 ?
3.2.1.26 levan + H2O
-
 Lacticaseibacillus paracasei D-fructose + ?
-
 ?
3.2.1.26 additional information no substrate: stachyose, melizitose Lacticaseibacillus paracasei ?
-
 ?
3.2.1.26 nystose + H2O
-
 Lacticaseibacillus paracasei D-fructose + ?
-
 ?
3.2.1.26 raffinose + H2O
-
 Lacticaseibacillus paracasei D-fructose + melibiose
-
 ?
3.2.1.26 sucrose + H2O
-
 Lacticaseibacillus paracasei beta-D-fructose + alpha-D-glucose
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.26 10 50 recombinant truncated cytosolic protein Lacticaseibacillus paracasei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.26 16
-
 raffinose pH 5.0, 37°C Lacticaseibacillus paracasei
3.2.1.26 64
-
 1-kestose pH 5.0, 37°C Lacticaseibacillus paracasei
3.2.1.26 84
-
 sucrose pH 5.0, 37°C Lacticaseibacillus paracasei
3.2.1.26 89
-
 levan pH 5.0, 37°C Lacticaseibacillus paracasei
3.2.1.26 95
-
 nystose pH 5.0, 37°C Lacticaseibacillus paracasei
3.2.1.26 106
-
 inulin pH 5.0, 37°C Lacticaseibacillus paracasei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.26 5
-
 recombinant truncated cytosolic protein Lacticaseibacillus paracasei