BRENDA - Enzyme Database show

Purification and inhibition studies with anions and sulfonamides of an alpha-carbonic anhydrase from the Antarctic seal Leptonychotes weddellii

Cincinelli, A.; Martellini, T.; Innocenti, A.; Scozzafava, A.; Supuran, C.T.; Bioorg. Med. Chem. 19, 1847-1851 (2011)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.1
acetazolamide
-
Leptonychotes weddellii
4.2.1.1
azide
-
Leptonychotes weddellii
4.2.1.1
benzolamide
-
Leptonychotes weddellii
4.2.1.1
bicarbonate
high inhibition
Leptonychotes weddellii
4.2.1.1
brinzolamide
-
Leptonychotes weddellii
4.2.1.1
bromide
-
Leptonychotes weddellii
4.2.1.1
Carbonate
high inhibition
Leptonychotes weddellii
4.2.1.1
chloride
-
Leptonychotes weddellii
4.2.1.1
Cyanate
high inhibition
Leptonychotes weddellii
4.2.1.1
cyanide
high inhibition
Leptonychotes weddellii
4.2.1.1
dichlorophenamide
-
Leptonychotes weddellii
4.2.1.1
dorzolamide
-
Leptonychotes weddellii
4.2.1.1
fluoride
-
Leptonychotes weddellii
4.2.1.1
H2NSO2NH2
-
Leptonychotes weddellii
4.2.1.1
H2NSO3H
-
Leptonychotes weddellii
4.2.1.1
HSO3-
-
Leptonychotes weddellii
4.2.1.1
hydrochlorothiazide
-
Leptonychotes weddellii
4.2.1.1
Iodide
-
Leptonychotes weddellii
4.2.1.1
methazolamide
-
Leptonychotes weddellii
4.2.1.1
additional information
no inhibition by perchlorate
Leptonychotes weddellii
4.2.1.1
nitrate
-
Leptonychotes weddellii
4.2.1.1
nitrite
-
Leptonychotes weddellii
4.2.1.1
PhAsO3H2
-
Leptonychotes weddellii
4.2.1.1
PhB(OH)2
-
Leptonychotes weddellii
4.2.1.1
saccharin
-
Leptonychotes weddellii
4.2.1.1
sulfamate
-
Leptonychotes weddellii
4.2.1.1
sulfamide
high inhibition
Leptonychotes weddellii
4.2.1.1
sulfate
-
Leptonychotes weddellii
4.2.1.1
Sulfide
-
Leptonychotes weddellii
4.2.1.1
thiocyanate
high inhibition
Leptonychotes weddellii
4.2.1.1
zonisamide
-
Leptonychotes weddellii
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.1
Zn2+
catalytically crucial
Leptonychotes weddellii
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.1
30000
-
x * 30000, about, SDS-PAGE
Leptonychotes weddellii
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.1
H2CO3
Leptonychotes weddellii
-
CO2 + H2O
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.1
Leptonychotes weddellii
-
adult female Weddell seals
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.1
heart
-
Leptonychotes weddellii
-
4.2.1.1
intestine
-
Leptonychotes weddellii
-
4.2.1.1
kidney
-
Leptonychotes weddellii
-
4.2.1.1
liver
-
Leptonychotes weddellii
-
4.2.1.1
lung
-
Leptonychotes weddellii
-
4.2.1.1
spleen
-
Leptonychotes weddellii
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.1
H2CO3
-
714462
Leptonychotes weddellii
CO2 + H2O
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.1
?
x * 30000, about, SDS-PAGE
Leptonychotes weddellii
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.2.1.1
20
-
assay at
Leptonychotes weddellii
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.1
7.5
-
assay at
Leptonychotes weddellii
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
4.2.1.1
0.046
-
pH 7.5, 20C, inhibition of CO2 hydration activity
Leptonychotes weddellii
PhB(OH)2
4.2.1.1
0.055
-
pH 7.5, 20C, inhibition of CO2 hydration activity
Leptonychotes weddellii
H2NSO3H
4.2.1.1
0.057
-
pH 7.5, 20C, inhibition of CO2 hydration activity
Leptonychotes weddellii
PhAsO3H2
4.2.1.1
0.06
-
pH 7.5, 20C, inhibition of CO2 hydration activity
Leptonychotes weddellii
Carbonate
4.2.1.1
0.07
-
pH 7.5, 20C, inhibition of CO2 hydration activity
Leptonychotes weddellii
cyanide
4.2.1.1
0.07
-
pH 7.5, 20C, inhibition of CO2 hydration activity
Leptonychotes weddellii
H2NSO2NH2
4.2.1.1
0.087
-
pH 7.5, 20C, inhibition of CO2 hydration activity
Leptonychotes weddellii
Cyanate
4.2.1.1
0.097
-
pH 7.5, 20C, inhibition of CO2 hydration activity
Leptonychotes weddellii
thiocyanate
4.2.1.1
0.1
-
pH 7.5, 20C, inhibition of CO2 hydration activity
Leptonychotes weddellii
bicarbonate
4.2.1.1
0.59
-
pH 7.5, 20C, inhibition of CO2 hydration activity
Leptonychotes weddellii
HSO3-
4.2.1.1
0.74
-
pH 7.5, 20C, inhibition of CO2 hydration activity
Leptonychotes weddellii
fluoride
4.2.1.1
0.82
-
pH 7.5, 20C, inhibition of CO2 hydration activity
Leptonychotes weddellii
sulfate
4.2.1.1
0.83
-
pH 7.5, 20C, inhibition of CO2 hydration activity
Leptonychotes weddellii
azide
4.2.1.1
0.89
-
pH 7.5, 20C, inhibition of CO2 hydration activity
Leptonychotes weddellii
chloride
4.2.1.1
0.9
-
pH 7.5, 20C, inhibition of CO2 hydration activity
Leptonychotes weddellii
nitrite
4.2.1.1
1
-
pH 7.5, 20C, inhibition of CO2 hydration activity
Leptonychotes weddellii
Sulfide
4.2.1.1
1.01
-
pH 7.5, 20C, inhibition of CO2 hydration activity
Leptonychotes weddellii
bromide
4.2.1.1
20
-
pH 7.5, 20C, inhibition of CO2 hydration activity
Leptonychotes weddellii
Iodide
4.2.1.1
55.6
-
pH 7.5, 20C, inhibition of CO2 hydration activity
Leptonychotes weddellii
nitrate
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
4.2.1.1
0.046
-
pH 7.5, 20C, inhibition of CO2 hydration activity
Leptonychotes weddellii
PhB(OH)2
4.2.1.1
0.055
-
pH 7.5, 20C, inhibition of CO2 hydration activity
Leptonychotes weddellii
H2NSO3H
4.2.1.1
0.057
-
pH 7.5, 20C, inhibition of CO2 hydration activity
Leptonychotes weddellii
PhAsO3H2
4.2.1.1
0.06
-
pH 7.5, 20C, inhibition of CO2 hydration activity
Leptonychotes weddellii
Carbonate
4.2.1.1
0.07
-
pH 7.5, 20C, inhibition of CO2 hydration activity
Leptonychotes weddellii
cyanide
4.2.1.1
0.07
-
pH 7.5, 20C, inhibition of CO2 hydration activity
Leptonychotes weddellii
H2NSO2NH2
4.2.1.1
0.087
-
pH 7.5, 20C, inhibition of CO2 hydration activity
Leptonychotes weddellii
Cyanate
4.2.1.1
0.097
-
pH 7.5, 20C, inhibition of CO2 hydration activity
Leptonychotes weddellii
thiocyanate
4.2.1.1
0.1
-
pH 7.5, 20C, inhibition of CO2 hydration activity
Leptonychotes weddellii
bicarbonate
4.2.1.1
0.59
-
pH 7.5, 20C, inhibition of CO2 hydration activity
Leptonychotes weddellii
HSO3-
4.2.1.1
0.74
-
pH 7.5, 20C, inhibition of CO2 hydration activity
Leptonychotes weddellii
fluoride
4.2.1.1
0.82
-
pH 7.5, 20C, inhibition of CO2 hydration activity
Leptonychotes weddellii
sulfate
4.2.1.1
0.83
-
pH 7.5, 20C, inhibition of CO2 hydration activity
Leptonychotes weddellii
azide
4.2.1.1
0.89
-
pH 7.5, 20C, inhibition of CO2 hydration activity
Leptonychotes weddellii
chloride
4.2.1.1
0.9
-
pH 7.5, 20C, inhibition of CO2 hydration activity
Leptonychotes weddellii
nitrite
4.2.1.1
1
-
pH 7.5, 20C, inhibition of CO2 hydration activity
Leptonychotes weddellii
Sulfide
4.2.1.1
1.01
-
pH 7.5, 20C, inhibition of CO2 hydration activity
Leptonychotes weddellii
bromide
4.2.1.1
20
-
pH 7.5, 20C, inhibition of CO2 hydration activity
Leptonychotes weddellii
Iodide
4.2.1.1
55.6
-
pH 7.5, 20C, inhibition of CO2 hydration activity
Leptonychotes weddellii
nitrate
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.1
acetazolamide
-
Leptonychotes weddellii
4.2.1.1
azide
-
Leptonychotes weddellii
4.2.1.1
benzolamide
-
Leptonychotes weddellii
4.2.1.1
bicarbonate
high inhibition
Leptonychotes weddellii
4.2.1.1
brinzolamide
-
Leptonychotes weddellii
4.2.1.1
bromide
-
Leptonychotes weddellii
4.2.1.1
Carbonate
high inhibition
Leptonychotes weddellii
4.2.1.1
chloride
-
Leptonychotes weddellii
4.2.1.1
Cyanate
high inhibition
Leptonychotes weddellii
4.2.1.1
cyanide
high inhibition
Leptonychotes weddellii
4.2.1.1
dichlorophenamide
-
Leptonychotes weddellii
4.2.1.1
dorzolamide
-
Leptonychotes weddellii
4.2.1.1
fluoride
-
Leptonychotes weddellii
4.2.1.1
H2NSO2NH2
-
Leptonychotes weddellii
4.2.1.1
H2NSO3H
-
Leptonychotes weddellii
4.2.1.1
HSO3-
-
Leptonychotes weddellii
4.2.1.1
hydrochlorothiazide
-
Leptonychotes weddellii
4.2.1.1
Iodide
-
Leptonychotes weddellii
4.2.1.1
methazolamide
-
Leptonychotes weddellii
4.2.1.1
additional information
no inhibition by perchlorate
Leptonychotes weddellii
4.2.1.1
nitrate
-
Leptonychotes weddellii
4.2.1.1
nitrite
-
Leptonychotes weddellii
4.2.1.1
PhAsO3H2
-
Leptonychotes weddellii
4.2.1.1
PhB(OH)2
-
Leptonychotes weddellii
4.2.1.1
saccharin
-
Leptonychotes weddellii
4.2.1.1
sulfamate
-
Leptonychotes weddellii
4.2.1.1
sulfamide
high inhibition
Leptonychotes weddellii
4.2.1.1
sulfate
-
Leptonychotes weddellii
4.2.1.1
Sulfide
-
Leptonychotes weddellii
4.2.1.1
thiocyanate
high inhibition
Leptonychotes weddellii
4.2.1.1
zonisamide
-
Leptonychotes weddellii
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.1
Zn2+
catalytically crucial
Leptonychotes weddellii
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.1
30000
-
x * 30000, about, SDS-PAGE
Leptonychotes weddellii
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.1
H2CO3
Leptonychotes weddellii
-
CO2 + H2O
-
-
r
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.1
heart
-
Leptonychotes weddellii
-
4.2.1.1
intestine
-
Leptonychotes weddellii
-
4.2.1.1
kidney
-
Leptonychotes weddellii
-
4.2.1.1
liver
-
Leptonychotes weddellii
-
4.2.1.1
lung
-
Leptonychotes weddellii
-
4.2.1.1
spleen
-
Leptonychotes weddellii
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.1
H2CO3
-
714462
Leptonychotes weddellii
CO2 + H2O
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.1
?
x * 30000, about, SDS-PAGE
Leptonychotes weddellii
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.2.1.1
20
-
assay at
Leptonychotes weddellii
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.1
7.5
-
assay at
Leptonychotes weddellii