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Literature summary extracted from

  • Yamabhai, M.; Buranabanyat, B.; Jaruseranee, N.; Songsiriritthigul, C.
    Efficient E. coli expression systems for the production of recombinant beta-mannanases and other bacterial extracellular enzymes (2011), Bioeng. Bugs, 2, 45-49.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.78 synthesis expression in Escherichia coli based on T7 RNA polymerase promoter and tac promoter systems. Both Escherichia coli OmpA signal peptide and native Bacillus signal peptide can be used efficiently for secretion of recombinant protein. Enzyme can be harvested from whole cell lysate, periplasmic extract or culture broth 4-20 h after induction by IPTG Bacillus subtilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.78 expression in Escherichia coli Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.78 Bacillus subtilis O05512
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.78 635
-
 pH 6.0, 60°C, substrate locust bean gum Bacillus subtilis
3.2.1.78 962
-
 pH 6.0, 60°C, substrate glucomannan Bacillus subtilis
3.2.1.78 2223
-
 pH 6.0, 60°C, substrate 1,4-beta-D-mannan Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.78 1,4-beta-D-mannan + H2O
-
 Bacillus subtilis ?
-
 ?
3.2.1.78 glucomannan + H2O
-
 Bacillus subtilis ?
-
 ?
3.2.1.78 locust bean gum + H2O
-
 Bacillus subtilis ? products are various manno-oligosaccharides of M2-M6 size and mannose ?
3.2.1.78 additional information no substrate: guar gum, copra meal Bacillus subtilis ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.78 60
-
-
 Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.78 50
-
 pH 6.0, stable for 30 min, incubation without substrate Bacillus subtilis
3.2.1.78 60
-
 hlaf-life 4 min Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.78 6
-
-
 Bacillus subtilis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.78 2 10 50°C, stable for 30 min Bacillus subtilis