EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.3.3.7 | purified recombinant Tm-DHDPS-DELTAArg-237, vapor diffusion method, mixing of 150 nl protein solution, containing 11.2 mg/ml protein in 20 mM Tris-HCl, pH 8.0, with 150 nl reservoir solution, containing 40% v/v PEG 300, 100 mM phosphate-citrate, buffer, pH 4.2, and 0.02% w/v sodium azide, X-ray diffraction structure determination and analysis at 1.9-2.1 A resolution | Thermotoga maritima |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.3.3.7 | additional information | construction of mutants Tm-DHDPS-DELTAAsp168, DELTAAsp171, or DELTAArg237 by mutating charged residues, reduction of the number of salt bridges at one of the two tetramerization interface of the enzyme and its interactions results in variants with altered quaternary structure, e.g. monomeric, as shown by analytical ultracentrifugation, gel filtration liquid chromatography, and small angle X-ray scattering, and X-ray crystallographic studies, overview | Thermotoga maritima |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.3.3.7 | additional information | - |
additional information | Michaelis-Menten kinetics for wild-type and mutant enzymes, overview | Thermotoga maritima | |
4.3.3.7 | 0.05 | - |
pyruvate | pH not specified in the publication, 30°C, recombinant mutant DELTAAsp237 | Thermotoga maritima | |
4.3.3.7 | 0.07 | - |
pyruvate | pH not specified in the publication, 30°C, recombinant mutant DELTAAsp171 | Thermotoga maritima | |
4.3.3.7 | 0.08 | - |
pyruvate | pH not specified in the publication, 30°C, recombinant mutant DELTAAsp171/Arg237 | Thermotoga maritima | |
4.3.3.7 | 0.08 | - |
pyruvate | pH not specified in the publication, 30°C, recombinant wild-type enzyme | Thermotoga maritima | |
4.3.3.7 | 0.1 | - |
pyruvate | pH not specified in the publication, 45°C, recombinant mutant DELTAAsp237 | Thermotoga maritima | |
4.3.3.7 | 0.15 | - |
L-aspartate 4-semialdehyde | pH not specified in the publication, 45°C, recombinant mutant DELTAAsp237 | Thermotoga maritima | |
4.3.3.7 | 0.15 | - |
pyruvate | pH not specified in the publication, 45°C, recombinant wild-type enzyme | Thermotoga maritima | |
4.3.3.7 | 0.16 | - |
pyruvate | pH not specified in the publication, 45°C, recombinant mutant DELTAAsp171 | Thermotoga maritima | |
4.3.3.7 | 0.18 | - |
L-aspartate 4-semialdehyde | pH not specified in the publication, 30°C, recombinant mutant DELTAAsp237 | Thermotoga maritima | |
4.3.3.7 | 0.18 | - |
pyruvate | pH not specified in the publication, 45°C, recombinant mutant DELTAAsp171/Arg237 | Thermotoga maritima | |
4.3.3.7 | 0.23 | - |
L-aspartate 4-semialdehyde | pH not specified in the publication, 30°C, recombinant mutant DELTAAsp171/Arg237 | Thermotoga maritima | |
4.3.3.7 | 0.23 | - |
L-aspartate 4-semialdehyde | pH not specified in the publication, 30°C, recombinant wild-type enzyme | Thermotoga maritima | |
4.3.3.7 | 0.32 | - |
L-aspartate 4-semialdehyde | pH not specified in the publication, 45°C, recombinant mutant DELTAAsp171/Arg237 | Thermotoga maritima | |
4.3.3.7 | 0.36 | - |
L-aspartate 4-semialdehyde | pH not specified in the publication, 45°C, recombinant wild-type enzyme | Thermotoga maritima | |
4.3.3.7 | 0.42 | - |
L-aspartate 4-semialdehyde | pH not specified in the publication, 30°C, recombinant mutant DELTAAsp171 | Thermotoga maritima | |
4.3.3.7 | 0.46 | - |
L-aspartate 4-semialdehyde | pH not specified in the publication, 45°C, recombinant mutant DELTAAsp171 | Thermotoga maritima | |
4.3.3.7 | 1.1 | - |
L-aspartate 4-semialdehyde | pH not specified in the publication, 45°C, recombinant mutant DELTAAsp168/Asp171 | Thermotoga maritima | |
4.3.3.7 | 1.2 | - |
L-aspartate 4-semialdehyde | pH not specified in the publication, 30°C, recombinant mutant DELTAAsp168/Asp237 | Thermotoga maritima | |
4.3.3.7 | 1.3 | - |
L-aspartate 4-semialdehyde | pH not specified in the publication, 30°C, recombinant mutant DELTAAsp168/Asp171 | Thermotoga maritima | |
4.3.3.7 | 1.5 | - |
L-aspartate 4-semialdehyde | pH not specified in the publication, 30°C, recombinant mutant DELTAAsp168 | Thermotoga maritima | |
4.3.3.7 | 1.5 | - |
pyruvate | pH not specified in the publication, 30°C, recombinant mutant DELTAAsp168/Asp171 | Thermotoga maritima | |
4.3.3.7 | 1.7 | - |
pyruvate | pH not specified in the publication, 30°C, recombinant mutant DELTAAsp168 | Thermotoga maritima | |
4.3.3.7 | 1.7 | - |
pyruvate | pH not specified in the publication, 30°C, recombinant mutant DELTAAsp168/Asp237 | Thermotoga maritima | |
4.3.3.7 | 1.7 | - |
L-aspartate 4-semialdehyde | pH not specified in the publication, 45°C, recombinant mutant DELTAAsp168/Asp237 | Thermotoga maritima | |
4.3.3.7 | 2.1 | - |
pyruvate | pH not specified in the publication, 45°C, recombinant mutant DELTAAsp168 | Thermotoga maritima | |
4.3.3.7 | 2.2 | - |
L-aspartate 4-semialdehyde | pH not specified in the publication, 45°C, recombinant mutant DELTAAsp168 | Thermotoga maritima | |
4.3.3.7 | 2.4 | - |
pyruvate | pH not specified in the publication, 45°C, recombinant mutant DELTAAsp168/Asp171 | Thermotoga maritima | |
4.3.3.7 | 2.6 | - |
pyruvate | pH not specified in the publication, 45°C, recombinant mutant DELTAAsp168/Asp237 | Thermotoga maritima |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.3.3.7 | 34000 | - |
4 * 34000, SDS-PAGE, role of quaternary structure in the TIM-barrel family of enzymes, overview. Unlike other DHDPS enzymes, but like many thermophilic enzymes, Tm-DHDPS has a large number of charged residues at the quaternary interface. Removal of electrostatic interactions disrupts quaternary structure | Thermotoga maritima |
4.3.3.7 | 130000 | - |
gel filtration | Thermotoga maritima |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.3.7 | L-aspartate 4-semialdehyde + pyruvate | Thermotoga maritima | - |
(S)-2,3-dihydropyridine-2,6-dicarboxylate + 2 H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.3.3.7 | Thermotoga maritima | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.3.7 | L-aspartate 4-semialdehyde + pyruvate | - |
Thermotoga maritima | (S)-2,3-dihydropyridine-2,6-dicarboxylate + 2 H2O | - |
? | |
4.3.3.7 | additional information | the active site of the monomer is well conserved, with most active-site residues in the same conformation | Thermotoga maritima | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.3.3.7 | tetramer | 4 * 34000, SDS-PAGE, role of quaternary structure in the TIM-barrel family of enzymes, overview. Unlike other DHDPS enzymes, but like many thermophilic enzymes, Tm-DHDPS has a large number of charged residues at the quaternary interface. Removal of electrostatic interactions disrupts quaternary structure | Thermotoga maritima |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.3.3.7 | DHDPS | - |
Thermotoga maritima |
4.3.3.7 | More | the enzyme belongs to the TIM-barrel family of enzymes | Thermotoga maritima |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.3.3.7 | 30 | 45 | assay at 30°C and at 45°C | Thermotoga maritima |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.3.3.7 | 35 | - |
pyruvate | pH not specified in the publication, 30°C, recombinant mutant DELTAAsp168 | Thermotoga maritima | |
4.3.3.7 | 35 | - |
L-aspartate 4-semialdehyde | pH not specified in the publication, 30°C, recombinant mutant DELTAAsp168 | Thermotoga maritima | |
4.3.3.7 | 39 | - |
pyruvate | pH not specified in the publication, 30°C, recombinant mutant DELTAAsp237 | Thermotoga maritima | |
4.3.3.7 | 39 | - |
L-aspartate 4-semialdehyde | pH not specified in the publication, 30°C, recombinant mutant DELTAAsp237 | Thermotoga maritima | |
4.3.3.7 | 52 | - |
pyruvate | pH not specified in the publication, 30°C, recombinant mutant DELTAAsp168/Asp171 | Thermotoga maritima | |
4.3.3.7 | 52 | - |
L-aspartate 4-semialdehyde | pH not specified in the publication, 30°C, recombinant mutant DELTAAsp168/Asp171 | Thermotoga maritima | |
4.3.3.7 | 63 | - |
pyruvate | pH not specified in the publication, 30°C, recombinant mutant DELTAAsp171/Arg237 | Thermotoga maritima | |
4.3.3.7 | 63 | - |
L-aspartate 4-semialdehyde | pH not specified in the publication, 30°C, recombinant mutant DELTAAsp171/Arg237 | Thermotoga maritima | |
4.3.3.7 | 68 | - |
pyruvate | pH not specified in the publication, 30°C, recombinant mutant DELTAAsp168/Asp237 | Thermotoga maritima | |
4.3.3.7 | 68 | - |
L-aspartate 4-semialdehyde | pH not specified in the publication, 30°C, recombinant mutant DELTAAsp168/Asp237 | Thermotoga maritima | |
4.3.3.7 | 88 | - |
pyruvate | pH not specified in the publication, 45°C, recombinant mutant DELTAAsp237 | Thermotoga maritima | |
4.3.3.7 | 88 | - |
L-aspartate 4-semialdehyde | pH not specified in the publication, 45°C, recombinant mutant DELTAAsp237 | Thermotoga maritima | |
4.3.3.7 | 97 | - |
pyruvate | pH not specified in the publication, 30°C, recombinant mutant DELTAAsp171 | Thermotoga maritima | |
4.3.3.7 | 97 | - |
L-aspartate 4-semialdehyde | pH not specified in the publication, 30°C, recombinant mutant DELTAAsp171 | Thermotoga maritima | |
4.3.3.7 | 108 | - |
pyruvate | pH not specified in the publication, 45°C, recombinant mutant DELTAAsp168/Asp171 | Thermotoga maritima | |
4.3.3.7 | 108 | - |
L-aspartate 4-semialdehyde | pH not specified in the publication, 45°C, recombinant mutant DELTAAsp168/Asp171 | Thermotoga maritima | |
4.3.3.7 | 111 | - |
pyruvate | pH not specified in the publication, 45°C, recombinant mutant DELTAAsp168 | Thermotoga maritima | |
4.3.3.7 | 111 | - |
L-aspartate 4-semialdehyde | pH not specified in the publication, 45°C, recombinant mutant DELTAAsp168 | Thermotoga maritima | |
4.3.3.7 | 134 | - |
pyruvate | pH not specified in the publication, 45°C, recombinant mutant DELTAAsp171/Arg237 | Thermotoga maritima | |
4.3.3.7 | 134 | - |
L-aspartate 4-semialdehyde | pH not specified in the publication, 45°C, recombinant mutant DELTAAsp171/Arg237 | Thermotoga maritima | |
4.3.3.7 | 136 | - |
pyruvate | pH not specified in the publication, 30°C, recombinant wild-type enzyme | Thermotoga maritima | |
4.3.3.7 | 136 | - |
L-aspartate 4-semialdehyde | pH not specified in the publication, 30°C, recombinant wild-type enzyme | Thermotoga maritima | |
4.3.3.7 | 141 | - |
pyruvate | pH not specified in the publication, 45°C, recombinant mutant DELTAAsp168/Asp237 | Thermotoga maritima | |
4.3.3.7 | 141 | - |
L-aspartate 4-semialdehyde | pH not specified in the publication, 45°C, recombinant mutant DELTAAsp168/Asp237 | Thermotoga maritima | |
4.3.3.7 | 233 | - |
pyruvate | pH not specified in the publication, 45°C, recombinant mutant DELTAAsp171 | Thermotoga maritima | |
4.3.3.7 | 233 | - |
L-aspartate 4-semialdehyde | pH not specified in the publication, 45°C, recombinant mutant DELTAAsp171 | Thermotoga maritima | |
4.3.3.7 | 465 | - |
pyruvate | pH not specified in the publication, 45°C, recombinant wild-type enzyme | Thermotoga maritima | |
4.3.3.7 | 465 | - |
L-aspartate 4-semialdehyde | pH not specified in the publication, 45°C, recombinant wild-type enzyme | Thermotoga maritima |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.3.3.7 | additional information | pyruvate binding occurs near the large interface of DHDPS, and is likely, therefore, to stabilize this solvent-accessible face, which favors the formation of a dimer rather than a monomer. For the DELTAAsp168/Arg237 and DELTAAsp168/Asp171 DHDPS variants addition of pyruvate shifts the equilibrium from primarily monomer to favor almost exclusively dimers. On the other hand, for the DELTAAsp168 DHDPS variant, the monomer-tetramer equilibrium shifts from primarily monomer to primarily tetramer on addition of pyruvate | Thermotoga maritima |