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Literature summary extracted from

  • Dilokpimol, A.; Nakai, H.; Gotfredsen, C.H.; Baumann, M.J.; Nakai, N.; Hachem, M.A.; Svensson, B.
    Recombinant production and characterisation of two related GH5 endo-beta-1,4-mannanases from Aspergillus nidulans FGSC A4 showing distinctly different transglycosylation capacity (2011), Biochim. Biophys. Acta, 1814, 1720-1729.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.78 expression in Pichia pastoris Aspergillus nidulans

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.78 S289W mutation lowers KM for mannooligosaccharides by 30-45% and increases transglycosylation yield by 50% compared to wild-type Aspergillus nidulans
3.2.1.78 W283S mutation in subsite +1, mutation results in increase in KM value, reduction in the transglycosylation yield by 30-45% and decrease in activity towards mannans Aspergillus nidulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.78 0.6
-
 Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man wild-type, pH 5.5, 37°C Aspergillus nidulans
3.2.1.78 1
-
 Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man mutant W283S, pH 5.5, 37°C Aspergillus nidulans
3.2.1.78 1.3
-
 Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man mutant W283S, pH 5.5, 37°C Aspergillus nidulans
3.2.1.78 1.6
-
 Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man mutant W283S, pH 5.5, 37°C Aspergillus nidulans
3.2.1.78 1.8
-
 Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man pH 5.5, 37°C Aspergillus nidulans
3.2.1.78 1.8
-
 Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man wild-type, pH 5.5, 37°C Aspergillus nidulans
3.2.1.78 2.8
-
 Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man mutant W283S, pH 5.5, 37°C Aspergillus nidulans
3.2.1.78 2.9
-
 Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man pH 5.5, 37°C Aspergillus nidulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.78 43847
-
 x * 56000, SDS-PAGE, x * 43847, calculated Aspergillus nidulans
3.2.1.78 46644
-
 x * 56000, SDS-PAGE, x * 46644, calculated Aspergillus nidulans
3.2.1.78 56000
-
 x * 56000, SDS-PAGE, x * 43847, calculated Aspergillus nidulans
3.2.1.78 56000
-
 x * 56000, SDS-PAGE, x * 46644, calculated Aspergillus nidulans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.78 Aspergillus nidulans Q5AZ53 member of glycosyl hydrolase family 5
-
3.2.1.78 Aspergillus nidulans Q5B7X2 member of glycosyl hydrolase family 5
-
3.2.1.78 Aspergillus nidulans FGSC A4 Q5AZ53 member of glycosyl hydrolase family 5
-
3.2.1.78 Aspergillus nidulans FGSC A4 Q5B7X2 member of glycosyl hydrolase family 5
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.78 glycoprotein treatment by endoglycosidase H decreases molecular mass to 46000 Da Aspergillus nidulans
3.2.1.78 glycoprotein treatment by endoglycosidase H decreases molecular mass to 51000 Da Aspergillus nidulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.78 guar gum glucomannan + H2O
-
 Aspergillus nidulans ?
-
 ?
3.2.1.78 guar gum glucomannan + H2O
-
 Aspergillus nidulans FGSC A4 ?
-
 ?
3.2.1.78 konjac glucomannan + H2O
-
 Aspergillus nidulans ?
-
 ?
3.2.1.78 konjac glucomannan + H2O
-
 Aspergillus nidulans FGSC A4 ?
-
 ?
3.2.1.78 locust bean gum glucomannan + H2O
-
 Aspergillus nidulans ?
-
 ?
3.2.1.78 locust bean gum glucomannan + H2O
-
 Aspergillus nidulans FGSC A4 ?
-
 ?
3.2.1.78 Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man + H2O increasing catalytic efficiency towards beta-1,4 mannooligosaccharides with the degree of polymerisation from 4 to 6 Aspergillus nidulans 2 Man-beta-1,4-Man 64% of product ?
3.2.1.78 Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man + H2O increasing catalytic efficiency towards beta-1,4-mannooligosaccharides with the degree of polymerisation from 4 to 6 Aspergillus nidulans 2 Man-beta-1,4-Man 68% of product ?
3.2.1.78 Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man + H2O increasing catalytic efficiency towards beta-1,4-mannooligosaccharides with the degree of polymerisation from 4 to 6 Aspergillus nidulans FGSC A4 2 Man-beta-1,4-Man 68% of product ?
3.2.1.78 Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man + H2O increasing catalytic efficiency towards beta-1,4 mannooligosaccharides with the degree of polymerisation from 4 to 6 Aspergillus nidulans FGSC A4 2 Man-beta-1,4-Man 64% of product ?
3.2.1.78 Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man + H2O increasing catalytic efficiency towards beta-1,4 mannooligosaccharides with the degree of polymerisation from 4 to 6 Aspergillus nidulans Man-beta-1,4-Man-beta-1,4-Man + Man-beta-1,4-Man sole products ?
3.2.1.78 Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man + H2O increasing catalytic efficiency towards beta-1,4-mannooligosaccharides with the degree of polymerisation from 4 to 6 Aspergillus nidulans Man-beta-1,4-Man-beta-1,4-Man + Man-beta-1,4-Man sole products ?
3.2.1.78 Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man + H2O increasing catalytic efficiency towards beta-1,4 mannooligosaccharides with the degree of polymerisation from 4 to 6 Aspergillus nidulans Man-beta-1,4-Man + Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man 88% of products ?
3.2.1.78 Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man + H2O increasing catalytic efficiency towards beta-1,4-mannooligosaccharides with the degree of polymerisation from 4 to 6 Aspergillus nidulans Man-beta-1,4-Man + Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man 88% of products ?
3.2.1.78 additional information enzyme displays good transgycosylation activity using mannotriose, melezitose and isomaltotriose as acceptors Aspergillus nidulans ?
-
 ?
3.2.1.78 additional information enzyme displays transglycosylation activity using only mannotriose as acceptor Aspergillus nidulans ?
-
 ?
3.2.1.78 additional information enzyme displays good transgycosylation activity using mannotriose, melezitose and isomaltotriose as acceptors Aspergillus nidulans FGSC A4 ?
-
 ?
3.2.1.78 additional information enzyme displays transglycosylation activity using only mannotriose as acceptor Aspergillus nidulans FGSC A4 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.78 ? x * 56000, SDS-PAGE, x * 43847, calculated Aspergillus nidulans
3.2.1.78 ? x * 56000, SDS-PAGE, x * 46644, calculated Aspergillus nidulans

Synonyms

EC Number Synonyms Comment Organism
3.2.1.78 ManA
-
 Aspergillus nidulans
3.2.1.78 ManC
-
 Aspergillus nidulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.78 50
-
-
 Aspergillus nidulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.78 40
-
 3 h, 95% residual activity Aspergillus nidulans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.78 2.8
-
 Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man mutant W283S, pH 5.5, 37°C Aspergillus nidulans
3.2.1.78 67
-
 Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man pH 5.5, 37°C Aspergillus nidulans
3.2.1.78 98
-
 Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man mutant W283S, pH 5.5, 37°C Aspergillus nidulans
3.2.1.78 112
-
 Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man wild-type, pH 5.5, 37°C Aspergillus nidulans
3.2.1.78 117
-
 Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man mutant W283S, pH 5.5, 37°C Aspergillus nidulans
3.2.1.78 134
-
 Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man wild-type, pH 5.5, 37°C Aspergillus nidulans
3.2.1.78 148
-
 Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man mutant W283S, pH 5.5, 37°C Aspergillus nidulans
3.2.1.78 193
-
 Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man pH 5.5, 37°C Aspergillus nidulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.78 5.5
-
-
 Aspergillus nidulans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.78 4.5 7 more than 95% of maximum activity Aspergillus nidulans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.78 23
-
 Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man pH 5.5, 37°C Aspergillus nidulans
3.2.1.78 27
-
 Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man mutant W283S, pH 5.5, 37°C Aspergillus nidulans
3.2.1.78 36
-
 Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man mutant W283S, pH 5.5, 37°C Aspergillus nidulans
3.2.1.78 61
-
 Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man wild-type, pH 5.5, 37°C Aspergillus nidulans
3.2.1.78 109
-
 Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man pH 5.5, 37°C Aspergillus nidulans
3.2.1.78 115
-
 Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man mutant W283S, pH 5.5, 37°C Aspergillus nidulans
3.2.1.78 118
-
 Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man mutant W283S, pH 5.5, 37°C Aspergillus nidulans
3.2.1.78 215
-
 Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man wild-type, pH 5.5, 37°C Aspergillus nidulans