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Literature summary extracted from

  • Baskaran, P.; Heckler, E.J.; van den Akker, F.; Beuve, A.
    Aspartate 102 in the heme domain of soluble guanylyl cyclase has a key role in NO activation (2011), Biochemistry, 50, 4291-4297.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.6.1.2 2-(N,N-diethylamino)diazenolate-2-oxide the wild type enzyme shows 59fold stimulation of activity at 0.001 mM 2-(N,N-diethylamino)diazenolate-2-oxide Rattus norvegicus
4.6.1.2 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol the wild type enzyme shows 6fold stimulation of activity at 0.1 mM Rattus norvegicus
4.6.1.2 protoporphyrin-IX the wild type enzyme shows 1.9fold stimulation of activity at 0.1 mM and 12fold stimulation at 0.01 mM Rattus norvegicus
4.6.1.2 S-nitroso-N-acetylpenicillamine the wild type enzyme shows 14.5fold stimulation of activity at 0.1 mM Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.2 expressed in Sf21 insect cells Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
4.6.1.2 D102A the mutant shows 70% of wild type activity Rattus norvegicus
4.6.1.2 D102E the mutant shows 32% of wild type activity Rattus norvegicus
4.6.1.2 D102N the mutant shows 20.5% of wild type activity Rattus norvegicus
4.6.1.2 F120A the mutant shows 73% of wild type activity Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.6.1.2 soluble
-
 Rattus norvegicus
-
-

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.2 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.2 cobalt affinity column chromatography and Mono Q anion exchange column chromatography Rattus norvegicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.6.1.2 0.047
-
 mutant enzyme D102N, at pH 8.5 and 33°C, in the absence of any stimulator Rattus norvegicus
4.6.1.2 0.073
-
 mutant enzyme D102E, at pH 8.5 and 33°C, in the absence of any stimulator Rattus norvegicus
4.6.1.2 0.087
-
 mutant enzyme D102N, at pH 8.5 and 33°C, in the presence of 0.1 mM protoporphyrin-IX Rattus norvegicus
4.6.1.2 0.108
-
 mutant enzyme F120A, at pH 8.5 and 33°C, in the presence of 0.1 mM protoporphyrin-IX Rattus norvegicus
4.6.1.2 0.128
-
 mutant enzyme D102E, at pH 8.5 and 33°C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol Rattus norvegicus
4.6.1.2 0.152
-
 mutant enzyme D102E, at pH 8.5 and 33°C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol Rattus norvegicus
4.6.1.2 0.16
-
 mutant enzyme D102A, at pH 8.5 and 33°C, in the presence of 0.1 mM protoporphyrin-IX Rattus norvegicus
4.6.1.2 0.161
-
 mutant enzyme D102A, at pH 8.5 and 33°C, in the absence of any stimulator Rattus norvegicus
4.6.1.2 0.168
-
 mutant enzyme D102E, at pH 8.5 and 33°C, in the presence of 0.1 mM protoporphyrin-IX Rattus norvegicus
4.6.1.2 0.169
-
 mutant enzyme F120A, at pH 8.5 and 33°C, in the absence of any stimulator Rattus norvegicus
4.6.1.2 0.172
-
 mutant enzyme F120A, at pH 8.5 and 33°C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol Rattus norvegicus
4.6.1.2 0.202
-
 mutant enzyme D102N, at pH 8.5 and 33°C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol Rattus norvegicus
4.6.1.2 0.229
-
 mutant enzyme D102N, at pH 8.5 and 33°C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol Rattus norvegicus
4.6.1.2 0.229
-
 wild type enzyme, at pH 8.5 and 33°C, in the absence of any stimulator Rattus norvegicus
4.6.1.2 0.231
-
 mutant enzyme F120A, at pH 8.5 and 33°C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol and 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol Rattus norvegicus
4.6.1.2 0.278
-
 mutant enzyme F120A, at pH 8.5 and 33°C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol Rattus norvegicus
4.6.1.2 0.292
-
 mutant enzyme D102E, at pH 8.5 and 33°C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol and 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol Rattus norvegicus
4.6.1.2 0.354
-
 mutant enzyme D102A, at pH 8.5 and 33°C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol Rattus norvegicus
4.6.1.2 0.431
-
 wild type enzyme, at pH 8.5 and 33°C, in the presence of 0.1 mM protoporphyrin-IX Rattus norvegicus
4.6.1.2 0.662
-
 mutant enzyme D102A, at pH 8.5 and 33°C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol and 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol Rattus norvegicus
4.6.1.2 1.063
-
 mutant enzyme D102N, at pH 8.5 and 33°C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol and 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol Rattus norvegicus
4.6.1.2 1.232
-
 mutant enzyme D102A, at pH 8.5 and 33°C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol Rattus norvegicus
4.6.1.2 1.43
-
 wild type enzyme, at pH 8.5 and 33°C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol Rattus norvegicus
4.6.1.2 3.328
-
 wild type enzyme, at pH 8.5 and 33°C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol Rattus norvegicus
4.6.1.2 3.402
-
 wild type enzyme, at pH 8.5 and 33°C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol and 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.2 GTP
-
 Rattus norvegicus 3',5'-cyclic-GMP + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.6.1.2 sGC
-
 Rattus norvegicus
4.6.1.2 soluble guanylate cyclase
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
4.6.1.2 heme
-
 Rattus norvegicus