EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.8.1 | expressed in Escherichia coli BL-21(DE3) cells | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.8.1 | F132G | the mutant shows decreased catalytic efficiency compared to the wild type enzyme | Homo sapiens |
3.1.8.1 | G60A | the mutant shows increased catalytic efficiency with sarin and soman compared to the wild type enzyme | Homo sapiens |
3.1.8.1 | H254Q/H257F | the mutant shows increased catalytic efficiency compared to the wild type enzyme | Homo sapiens |
3.1.8.1 | H257Y/L303T | the mutant shows decreased catalytic efficiency compared to the wild type enzyme | Homo sapiens |
3.1.8.1 | I106A/F132A/H257Y | the mutant shows decreased catalytic efficiency compared to the wild type enzyme | Homo sapiens |
3.1.8.1 | I106A/H257Y/S308A | the mutant shows decreased catalytic efficiency compared to the wild type enzyme | Homo sapiens |
3.1.8.1 | I106G | the mutant shows decreased catalytic efficiency compared to the wild type enzyme | Homo sapiens |
3.1.8.1 | I106G/F132G/H257Y | the mutant shows decreased catalytic efficiency compared to the wild type enzyme | Homo sapiens |
3.1.8.1 | I106G/H257Y | the mutant shows decreased catalytic efficiency compared to the wild type enzyme | Homo sapiens |
3.1.8.1 | S308G | the mutant shows decreased catalytic efficiency compared to the wild type enzyme | Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.8.1 | Zn2+ | the native enzyme contains two Zn2+ ions, and these metal ions can be substituted with Cd2+, Co2+, Ni2+, or Mn2+ without a loss of catalytic activity | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.8.1 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.8.1 | ammonium sulfate precipitation, Ultrogel AcA 54 gel filtration, and DEAE-Sephadex A-25 gel filtration | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.8.1 | 4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate + H2O | - |
Homo sapiens | 4-acetylphenol + (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate | - |
? | |
3.1.8.1 | 4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate + H2O | - |
Homo sapiens | 4-acetylphenol + (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | - |
? | |
3.1.8.1 | 4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (R)-methylphosphonate + H2O | - |
Homo sapiens | 4-acetylphenol + 4-acetylphenol + (2S)-3,3-dimethylbutan-2-yl (R)-methylphosphonate | - |
? | |
3.1.8.1 | 4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate + H2O | - |
Homo sapiens | 4-acetylphenol + (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | - |
? | |
3.1.8.1 | 4-acetylphenyl 2-methylpropyl (R)-methylphosphonate + H2O | - |
Homo sapiens | 4-acetylphenol + 2-methylpropyl (R)-methylphosphonate | - |
? | |
3.1.8.1 | 4-acetylphenyl 2-methylpropyl (S)-methylphosphonate + H2O | - |
Homo sapiens | 4-acetylphenol + 2-methylpropyl (S)-methylphosphonate | - |
? | |
3.1.8.1 | 4-acetylphenyl cyclohexyl (R)-methylphosphonate + H2O | - |
Homo sapiens | 4-acetylphenol + cyclohexyl (R)-methylphosphonate | - |
? | |
3.1.8.1 | 4-acetylphenyl cyclohexyl (S)-methylphosphonate + H2O | - |
Homo sapiens | 4-acetylphenol + cyclohexyl (S)-methylphosphonate | - |
? | |
3.1.8.1 | 4-acetylphenyl ethyl (R)-methylphosphonate + H2O | - |
Homo sapiens | 4-acetylphenol + ethyl (R)-methylphosphonate | - |
? | |
3.1.8.1 | 4-acetylphenyl ethyl (S)-methylphosphonate + H2O | - |
Homo sapiens | 4-acetylphenol + ethyl (S)-methylphosphonate | - |
? | |
3.1.8.1 | 4-acetylphenyl propan-2-yl (R)-methylphosphonate + H2O | - |
Homo sapiens | 4-acetylphenol + propan-2-yl (R)-methylphosphonate | - |
? | |
3.1.8.1 | 4-acetylphenyl propan-2-yl (S)-methylphosphonate + H2O | - |
Homo sapiens | 4-acetylphenol + propan-2-yl (S)-methylphosphonate | - |
? | |
3.1.8.1 | cyclosarin + H2O | - |
Homo sapiens | ? | - |
? | |
3.1.8.1 | additional information | the stereochemical preference of the wild-type enzyme with chemical warfare agents is for the RP enantiomers | Homo sapiens | ? | - |
? | |
3.1.8.1 | paraoxon + H2O | best substrate | Homo sapiens | 4-nitrophenol + diethyl phosphate | - |
? | |
3.1.8.1 | sarin + H2O | - |
Homo sapiens | ? | - |
? | |
3.1.8.1 | soman + H2O | - |
Homo sapiens | ? | - |
? | |
3.1.8.1 | tabun + H2O | - |
Homo sapiens | ? | - |
? | |
3.1.8.1 | VR + H2O | - |
Homo sapiens | ? | - |
? | |
3.1.8.1 | VX + H2O | - |
Homo sapiens | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.8.1 | homodimer | - |
Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.8.1 | phosphotriesterase | - |
Homo sapiens |
3.1.8.1 | PTE | - |
Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.8.1 | 0.016 | - |
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 0.12 | - |
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 0.14 | - |
4-acetylphenyl cyclohexyl (S)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 0.21 | - |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 0.41 | - |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 0.45 | - |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 0.62 | - |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 0.81 | - |
4-acetylphenyl cyclohexyl (R)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 1.5 | - |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 2 | - |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 2.1 | - |
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 2.2 | - |
soman | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 2.2 | - |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 2.6 | - |
soman | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 2.9 | - |
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 3.4 | - |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 5.1 | - |
4-acetylphenyl cyclohexyl (S)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 5.9 | - |
4-acetylphenyl cyclohexyl (R)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 7.3 | - |
4-acetylphenyl ethyl (R)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 7.4 | - |
4-acetylphenyl propan-2-yl (S)-methylphosphonate | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 8.5 | - |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 9.1 | - |
soman | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 12 | - |
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 14 | - |
4-acetylphenyl ethyl (R)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 15 | - |
sarin | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 15 | - |
4-acetylphenyl propan-2-yl (S)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 18 | - |
4-acetylphenyl propan-2-yl (R)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 19 | - |
4-acetylphenyl cyclohexyl (S)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 20 | - |
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 22 | - |
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 25 | - |
soman | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 37 | - |
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 39 | - |
sarin | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 40 | - |
4-acetylphenyl propan-2-yl (S)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 50 | - |
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 51 | - |
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 89 | - |
soman | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 90 | - |
sarin | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 92 | - |
4-acetylphenyl propan-2-yl (S)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 93 | - |
4-acetylphenyl cyclohexyl (R)-methylphosphonate | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 93 | - |
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 100 | - |
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 100 | - |
4-acetylphenyl propan-2-yl (R)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 110 | - |
4-acetylphenyl ethyl (S)-methylphosphonate | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 110 | - |
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 110 | - |
4-acetylphenyl ethyl (R)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 120 | - |
sarin | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 120 | - |
4-acetylphenyl propan-2-yl (R)-methylphosphonate | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 130 | - |
4-acetylphenyl ethyl (R)-methylphosphonate | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 150 | - |
4-acetylphenyl cyclohexyl (R)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 150 | - |
4-acetylphenyl ethyl (R)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 190 | - |
4-acetylphenyl ethyl (S)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 250 | - |
4-acetylphenyl propan-2-yl (R)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 290 | - |
4-acetylphenyl ethyl (S)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 300 | - |
sarin | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 370 | - |
4-acetylphenyl propan-2-yl (S)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 410 | - |
4-acetylphenyl ethyl (S)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 670 | - |
4-acetylphenyl ethyl (S)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.8.1 | 0.00094 | - |
4-acetylphenyl cyclohexyl (S)-methylphosphonate | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 0.0011 | - |
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 0.003 | - |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 0.0032 | - |
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 0.0067 | - |
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 0.016 | - |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 0.021 | - |
4-acetylphenyl cyclohexyl (S)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 0.058 | - |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 0.099 | - |
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 0.1 | - |
4-acetylphenyl cyclohexyl (S)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 0.13 | - |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 0.2 | - |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 0.22 | - |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 0.25 | - |
4-acetylphenyl cyclohexyl (R)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 0.25 | - |
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 0.49 | - |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 0.59 | - |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 1.3 | - |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 1.5 | - |
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 1.5 | - |
4-acetylphenyl ethyl (R)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 1.7 | - |
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 1.8 | - |
4-acetylphenyl propan-2-yl (R)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 1.9 | - |
4-acetylphenyl cyclohexyl (R)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 2.3 | - |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 2.4 | - |
4-acetylphenyl ethyl (R)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 2.5 | - |
4-acetylphenyl propan-2-yl (R)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 3.1 | - |
4-acetylphenyl propan-2-yl (S)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 5.8 | - |
4-acetylphenyl cyclohexyl (S)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 5.9 | - |
4-acetylphenyl propan-2-yl (S)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 6.2 | - |
4-acetylphenyl propan-2-yl (S)-methylphosphonate | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 9.5 | - |
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 13 | - |
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 16 | - |
4-acetylphenyl cyclohexyl (R)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 21 | - |
4-acetylphenyl cyclohexyl (R)-methylphosphonate | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 27 | - |
4-acetylphenyl propan-2-yl (S)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 28 | - |
4-acetylphenyl cyclohexyl (S)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 28 | - |
4-acetylphenyl cyclohexyl (R)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 34 | - |
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 73 | - |
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 75 | - |
4-acetylphenyl ethyl (R)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 110 | - |
4-acetylphenyl propan-2-yl (S)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 110 | - |
4-acetylphenyl propan-2-yl (R)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 130 | - |
4-acetylphenyl ethyl (S)-methylphosphonate | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 150 | - |
4-acetylphenyl ethyl (S)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 160 | - |
4-acetylphenyl ethyl (S)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 180 | - |
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 180 | - |
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 220 | - |
4-acetylphenyl ethyl (S)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 490 | - |
4-acetylphenyl ethyl (R)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 520 | - |
4-acetylphenyl ethyl (R)-methylphosphonate | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 520 | - |
4-acetylphenyl propan-2-yl (R)-methylphosphonate | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 580 | - |
4-acetylphenyl propan-2-yl (R)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 760 | - |
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 850 | - |
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1.8.1 | 1200 | - |
4-acetylphenyl ethyl (S)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens |