Literature summary extracted from

  • Tsai, P.C.; Bigley, A.; Li, Y.; Ghanem, E.; Cadieux, C.L.; Kasten, S.A.; Reeves, T.E.; Cerasoli, D.M.; Raushel, F.M.
    Stereoselective hydrolysis of organophosphate nerve agents by the bacterial phosphotriesterase (2010), Biochemistry, 49, 7978-7987.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.8.1 expressed in Escherichia coli BL-21(DE3) cells Homo sapiens

Engineering

EC Number Protein Variants Comment Organism
3.1.8.1 S308G the mutant shows decreased catalytic efficiency compared to the wild type enzyme Homo sapiens
3.1.8.1 G60A the mutant shows increased catalytic efficiency with sarin and soman compared to the wild type enzyme Homo sapiens
3.1.8.1 I106G the mutant shows decreased catalytic efficiency compared to the wild type enzyme Homo sapiens
3.1.8.1 F132G the mutant shows decreased catalytic efficiency compared to the wild type enzyme Homo sapiens
3.1.8.1 H254Q/H257F the mutant shows increased catalytic efficiency compared to the wild type enzyme Homo sapiens
3.1.8.1 H257Y/L303T the mutant shows decreased catalytic efficiency compared to the wild type enzyme Homo sapiens
3.1.8.1 I106G/H257Y the mutant shows decreased catalytic efficiency compared to the wild type enzyme Homo sapiens
3.1.8.1 I106G/F132G/H257Y the mutant shows decreased catalytic efficiency compared to the wild type enzyme Homo sapiens
3.1.8.1 I106A/F132A/H257Y the mutant shows decreased catalytic efficiency compared to the wild type enzyme Homo sapiens
3.1.8.1 I106A/H257Y/S308A the mutant shows decreased catalytic efficiency compared to the wild type enzyme Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.8.1 Zn2+ the native enzyme contains two Zn2+ ions, and these metal ions can be substituted with Cd2+, Co2+, Ni2+, or Mn2+ without a loss of catalytic activity Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.8.1 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.8.1 ammonium sulfate precipitation, Ultrogel AcA 54 gel filtration, and DEAE-Sephadex A-25 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.8.1 4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate + H2O
-
Homo sapiens 4-acetylphenol + (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate
-
?
3.1.8.1 4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate + H2O
-
Homo sapiens 4-acetylphenol + (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate
-
?
3.1.8.1 4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (R)-methylphosphonate + H2O
-
Homo sapiens 4-acetylphenol + 4-acetylphenol + (2S)-3,3-dimethylbutan-2-yl (R)-methylphosphonate
-
?
3.1.8.1 4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate + H2O
-
Homo sapiens 4-acetylphenol + (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate
-
?
3.1.8.1 4-acetylphenyl 2-methylpropyl (R)-methylphosphonate + H2O
-
Homo sapiens 4-acetylphenol + 2-methylpropyl (R)-methylphosphonate
-
?
3.1.8.1 4-acetylphenyl 2-methylpropyl (S)-methylphosphonate + H2O
-
Homo sapiens 4-acetylphenol + 2-methylpropyl (S)-methylphosphonate
-
?
3.1.8.1 4-acetylphenyl cyclohexyl (R)-methylphosphonate + H2O
-
Homo sapiens 4-acetylphenol + cyclohexyl (R)-methylphosphonate
-
?
3.1.8.1 4-acetylphenyl cyclohexyl (S)-methylphosphonate + H2O
-
Homo sapiens 4-acetylphenol + cyclohexyl (S)-methylphosphonate
-
?
3.1.8.1 4-acetylphenyl ethyl (R)-methylphosphonate + H2O
-
Homo sapiens 4-acetylphenol + ethyl (R)-methylphosphonate
-
?
3.1.8.1 4-acetylphenyl ethyl (S)-methylphosphonate + H2O
-
Homo sapiens 4-acetylphenol + ethyl (S)-methylphosphonate
-
?
3.1.8.1 4-acetylphenyl propan-2-yl (R)-methylphosphonate + H2O
-
Homo sapiens 4-acetylphenol + propan-2-yl (R)-methylphosphonate
-
?
3.1.8.1 4-acetylphenyl propan-2-yl (S)-methylphosphonate + H2O
-
Homo sapiens 4-acetylphenol + propan-2-yl (S)-methylphosphonate
-
?
3.1.8.1 cyclosarin + H2O
-
Homo sapiens ?
-
?
3.1.8.1 additional information the stereochemical preference of the wild-type enzyme with chemical warfare agents is for the RP enantiomers Homo sapiens ?
-
?
3.1.8.1 paraoxon + H2O best substrate Homo sapiens 4-nitrophenol + diethyl phosphate
-
?
3.1.8.1 sarin + H2O
-
Homo sapiens ?
-
?
3.1.8.1 soman + H2O
-
Homo sapiens ?
-
?
3.1.8.1 tabun + H2O
-
Homo sapiens ?
-
?
3.1.8.1 VR + H2O
-
Homo sapiens ?
-
?
3.1.8.1 VX + H2O
-
Homo sapiens ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.8.1 homodimer
-
Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.1.8.1 phosphotriesterase
-
Homo sapiens
3.1.8.1 PTE
-
Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.8.1 0.016
-
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 0.12
-
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 0.14
-
4-acetylphenyl cyclohexyl (S)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 0.21
-
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 0.41
-
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 0.45
-
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 0.62
-
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 0.81
-
4-acetylphenyl cyclohexyl (R)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 1.5
-
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 2
-
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 2.1
-
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 2.2
-
soman mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 2.2
-
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 2.6
-
soman wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 2.9
-
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 3.4
-
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 5.1
-
4-acetylphenyl cyclohexyl (S)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 5.9
-
4-acetylphenyl cyclohexyl (R)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 7.3
-
4-acetylphenyl ethyl (R)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 7.4
-
4-acetylphenyl propan-2-yl (S)-methylphosphonate mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 8.5
-
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 9.1
-
soman mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 12
-
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 14
-
4-acetylphenyl ethyl (R)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 15
-
sarin mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 15
-
4-acetylphenyl propan-2-yl (S)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 18
-
4-acetylphenyl propan-2-yl (R)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 19
-
4-acetylphenyl cyclohexyl (S)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 20
-
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 22
-
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 25
-
soman mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 37
-
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 39
-
sarin mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 40
-
4-acetylphenyl propan-2-yl (S)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 50
-
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 51
-
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 89
-
soman mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 90
-
sarin wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 92
-
4-acetylphenyl propan-2-yl (S)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 93
-
4-acetylphenyl cyclohexyl (R)-methylphosphonate mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 93
-
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 100
-
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 100
-
4-acetylphenyl propan-2-yl (R)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 110
-
4-acetylphenyl ethyl (S)-methylphosphonate mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 110
-
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 110
-
4-acetylphenyl ethyl (R)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 120
-
sarin mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 120
-
4-acetylphenyl propan-2-yl (R)-methylphosphonate mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 130
-
4-acetylphenyl ethyl (R)-methylphosphonate mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 150
-
4-acetylphenyl cyclohexyl (R)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 150
-
4-acetylphenyl ethyl (R)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 190
-
4-acetylphenyl ethyl (S)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 250
-
4-acetylphenyl propan-2-yl (R)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 290
-
4-acetylphenyl ethyl (S)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 300
-
sarin mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 370
-
4-acetylphenyl propan-2-yl (S)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 410
-
4-acetylphenyl ethyl (S)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 670
-
4-acetylphenyl ethyl (S)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens

KCat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.8.1 16
-
4-acetylphenyl cyclohexyl (R)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 2.5
-
4-acetylphenyl propan-2-yl (R)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 130
-
4-acetylphenyl ethyl (S)-methylphosphonate mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 6.2
-
4-acetylphenyl propan-2-yl (S)-methylphosphonate mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 5.9
-
4-acetylphenyl propan-2-yl (S)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 0.2
-
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 110
-
4-acetylphenyl propan-2-yl (S)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 110
-
4-acetylphenyl propan-2-yl (R)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 2.4
-
4-acetylphenyl ethyl (R)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 1200
-
4-acetylphenyl ethyl (S)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 5.8
-
4-acetylphenyl cyclohexyl (S)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 28
-
4-acetylphenyl cyclohexyl (S)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 28
-
4-acetylphenyl cyclohexyl (R)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 1.5
-
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 1.5
-
4-acetylphenyl ethyl (R)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 1.8
-
4-acetylphenyl propan-2-yl (R)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 2.3
-
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 0.49
-
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 0.021
-
4-acetylphenyl cyclohexyl (S)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 0.22
-
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 520
-
4-acetylphenyl ethyl (R)-methylphosphonate mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 520
-
4-acetylphenyl propan-2-yl (R)-methylphosphonate mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 1.3
-
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 0.13
-
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 21
-
4-acetylphenyl cyclohexyl (R)-methylphosphonate mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 0.003
-
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 3.1
-
4-acetylphenyl propan-2-yl (S)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 0.0032
-
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 150
-
4-acetylphenyl ethyl (S)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 34
-
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 0.016
-
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 0.0011
-
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 160
-
4-acetylphenyl ethyl (S)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 0.0067
-
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 0.25
-
4-acetylphenyl cyclohexyl (R)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 0.25
-
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 13
-
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 0.1
-
4-acetylphenyl cyclohexyl (S)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 180
-
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 180
-
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 1.7
-
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 0.59
-
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 1.9
-
4-acetylphenyl cyclohexyl (R)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 220
-
4-acetylphenyl ethyl (S)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 75
-
4-acetylphenyl ethyl (R)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 27
-
4-acetylphenyl propan-2-yl (S)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 490
-
4-acetylphenyl ethyl (R)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 9.5
-
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 73
-
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 760
-
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 0.00094
-
4-acetylphenyl cyclohexyl (S)-methylphosphonate mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 580
-
4-acetylphenyl propan-2-yl (R)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 850
-
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 0.058
-
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1.8.1 0.099
-
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens