Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hillar, A.; Peters, B.; Pauls, R.; Loboda, A.; Zhang, H.; Mauk, A.G.; Loewen, P.C.
    Modulation of the activities of catalase-peroxidase HPI of Escherichia coli by site-directed mutagenesis (2000), Biochemistry, 39, 5868-5875.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.21 H106C the peroxidatic-to-catalatic ratio of the mutant is increased 125fold, the heme content of the mutant is reduced relative to the wild type enzyme Escherichia coli
1.11.1.21 H106Y the peroxidatic-to-catalatic ratio of the mutant is increased 321fold, the heme content of the mutant is reduced relative to the wild type enzyme Escherichia coli
1.11.1.21 H267Y the peroxidatic-to-catalatic ratio of the mutant is increased 36fold, the heme content of the mutant is reduced relative to the wild type enzyme Escherichia coli
1.11.1.21 R102C the peroxidatic-to-catalatic ratio of the mutant is increased 18fold, the heme content of the mutant is reduced relative to the wild type enzyme Escherichia coli
1.11.1.21 R102K the peroxidatic-to-catalatic ratio of the mutant is increased 7fold, the heme content of the mutant is reduced relative to the wild type enzyme Escherichia coli
1.11.1.21 R102L the peroxidatic-to-catalatic ratio of the mutant is increased 13fold, the heme content of the mutant is reduced relative to the wild type enzyme Escherichia coli
1.11.1.21 W105F the peroxidatic-to-catalatic ratio of the mutant is increased 2800fold, the heme content of the mutant is reduced relative to the wild type enzyme Escherichia coli
1.11.1.21 W105L the peroxidatic-to-catalatic ratio of the mutant is increased 612fold, the heme content of the mutant is reduced relative to the wild type enzyme Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.21 azide
-
 Escherichia coli
1.11.1.21 cyanide
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.21 0.048
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase activity, mutant enzyme R102L, at pH 6.0 and 25°C Escherichia coli
1.11.1.21 0.055
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase activity, wild type enzyme, at pH 6.0 and 25°C Escherichia coli
1.11.1.21 0.08
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase activity, mutant enzyme H106C, at pH 6.0 and 25°C Escherichia coli
1.11.1.21 0.1
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase activity, mutant enzyme R102C, at pH 6.0 and 25°C Escherichia coli
1.11.1.21 0.24
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase activity, mutant enzyme W105L, at pH 6.0 and 25°C Escherichia coli
1.11.1.21 0.33
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase activity, mutant enzyme H106Y, at pH 6.0 and 25°C Escherichia coli
1.11.1.21 0.4
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase activity, mutant enzyme R102K, at pH 6.0 and 25°C Escherichia coli
1.11.1.21 0.48
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase activity, mutant enzyme W105F, at pH 6.0 and 25°C Escherichia coli
1.11.1.21 5.9
-
 H2O2 catalase activity, wild type enzyme, at pH 7.0 and 37°C Escherichia coli
1.11.1.21 12
-
 H2O2 catalase activity, mutant enzyme R102K, at pH 7.0 and 37°C Escherichia coli
1.11.1.21 28
-
 H2O2 catalase activity, mutant enzyme H106C, at pH 7.0 and 37°C Escherichia coli
1.11.1.21 31
-
 H2O2 catalase activity, mutant enzyme R102C, at pH 7.0 and 37°C Escherichia coli
1.11.1.21 33
-
 H2O2 catalase activity, mutant enzyme R102L, at pH 7.0 and 37°C Escherichia coli
1.11.1.21 53
-
 H2O2 catalase activity, mutant enzyme H267Y, at pH 7.0 and 37°C Escherichia coli
1.11.1.21 77
-
 H2O2 catalase activity, mutant enzyme W105L, at pH 7.0 and 37°C Escherichia coli
1.11.1.21 91
-
 H2O2 catalase activity, mutant enzyme W105F, at pH 7.0 and 37°C Escherichia coli
1.11.1.21 100
-
 H2O2 catalase activity, mutant enzyme H106Y, at pH 7.0 and 37°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.21 Escherichia coli
-
-
-
1.11.1.21 Escherichia coli UM262
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.21 0.16
-
 catalase activity, mutant enzyme H106Y, at pH 7.0 and 37°C Escherichia coli
1.11.1.21 0.25
-
 catalase activity, mutant enzyme H106C, at pH 7.0 and 37°C Escherichia coli
1.11.1.21 0.95
-
 catalase activity, mutant enzyme H267Y, at pH 7.0 and 37°C Escherichia coli
1.11.1.21 1.9
-
 catalase activity, mutant enzyme W105F, at pH 7.0 and 37°C Escherichia coli
1.11.1.21 5.9
-
 catalase activity, mutant enzyme W105L, at pH 7.0 and 37°C Escherichia coli
1.11.1.21 10
-
 catalase activity, mutant enzyme R102C, at pH 7.0 and 37°C Escherichia coli
1.11.1.21 11
-
 peroxidase activity, mutant enzyme H106C, at pH 7.0 and 37°C Escherichia coli
1.11.1.21 12
-
 peroxidase activity, mutant enzyme H267Y, at pH 7.0 and 37°C Escherichia coli
1.11.1.21 18
-
 peroxidase activity, mutant enzyme H106Y, at pH 7.0 and 37°C Escherichia coli
1.11.1.21 19
-
 catalase activity, mutant enzyme R102L, at pH 7.0 and 37°C Escherichia coli
1.11.1.21 35
-
 catalase activity, mutant enzyme R102K, at pH 7.0 and 37°C Escherichia coli
1.11.1.21 63
-
 peroxidase activity, mutant enzyme R102C, at pH 7.0 and 37°C Escherichia coli
1.11.1.21 85
-
 peroxidase activity, mutant enzyme R102K, at pH 7.0 and 37°C Escherichia coli
1.11.1.21 85
-
 peroxidase activity, mutant enzyme R102L, at pH 7.0 and 37°C Escherichia coli
1.11.1.21 660
-
 peroxidase activity, wild type enzyme, at pH 7.0 and 37°C Escherichia coli
1.11.1.21 1300
-
 peroxidase activity, mutant enzyme W105L, at pH 7.0 and 37°C Escherichia coli
1.11.1.21 1900
-
 catalase activity, wild type enzyme, at pH 7.0 and 37°C Escherichia coli
1.11.1.21 1900
-
 peroxidase activity, mutant enzyme W105F, at pH 7.0 and 37°C Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.21 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
 Escherichia coli oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
 ?
1.11.1.21 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
 Escherichia coli UM262 oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
 ?
1.11.1.21 H2O2
-
 Escherichia coli O2 + H2O
-
 ?
1.11.1.21 H2O2
-
 Escherichia coli UM262 O2 + H2O
-
 ?
1.11.1.21 isoniazid + H2O2
-
 Escherichia coli oxidized isoniazid + H2O
-
 ?
1.11.1.21 isoniazid + H2O2
-
 Escherichia coli UM262 oxidized isoniazid + H2O
-
 ?
1.11.1.21 o-dianisidine + H2O2
-
 Escherichia coli oxidized o-dianisidine + H2O
-
 ?
1.11.1.21 o-dianisidine + H2O2
-
 Escherichia coli UM262 oxidized o-dianisidine + H2O
-
 ?
1.11.1.21 tert-butyl hydroperoxide + H2O2
-
 Escherichia coli oxidized tert-butyl hydroperoxide + H2O
-
 ?
1.11.1.21 tert-butyl hydroperoxide + H2O2
-
 Escherichia coli UM262 oxidized tert-butyl hydroperoxide + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.21 HPI
-
 Escherichia coli
1.11.1.21 hydroperoxidase I
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.21 0.0061
-
 H2O2 catalase activity, mutant enzyme H106Y, at pH 7.0 and 37°C Escherichia coli
1.11.1.21 0.089
-
 H2O2 catalase activity, mutant enzyme H106C, at pH 7.0 and 37°C Escherichia coli
1.11.1.21 1.1
-
 H2O2 catalase activity, mutant enzyme W105F, at pH 7.0 and 37°C Escherichia coli
1.11.1.21 1.1
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase activity, mutant enzyme H106Y, at pH 6.0 and 25°C Escherichia coli
1.11.1.21 1.6
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase activity, mutant enzyme H106C, at pH 6.0 and 25°C Escherichia coli
1.11.1.21 2.1
-
 H2O2 catalase activity, mutant enzyme R102K, at pH 7.0 and 37°C Escherichia coli
1.11.1.21 2.6
-
 H2O2 catalase activity, mutant enzyme H267Y, at pH 7.0 and 37°C Escherichia coli
1.11.1.21 23
-
 H2O2 catalase activity, mutant enzyme W105L, at pH 7.0 and 37°C Escherichia coli
1.11.1.21 53.5
-
 H2O2 catalase activity, mutant enzyme R102C, at pH 7.0 and 37°C Escherichia coli
1.11.1.21 660
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase activity, mutant enzyme R102L, at pH 6.0 and 25°C Escherichia coli
1.11.1.21 710
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase activity, mutant enzyme R102C, at pH 6.0 and 25°C Escherichia coli
1.11.1.21 900
-
 H2O2 catalase activity, mutant enzyme R102L, at pH 7.0 and 37°C Escherichia coli
1.11.1.21 3700
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase activity, mutant enzyme R102K, at pH 6.0 and 25°C Escherichia coli
1.11.1.21 5300
-
 H2O2 catalase activity, wild type enzyme, at pH 7.0 and 37°C Escherichia coli
1.11.1.21 17000
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase activity, wild type enzyme, at pH 6.0 and 25°C Escherichia coli
1.11.1.21 140000
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase activity, mutant enzyme W105L, at pH 6.0 and 25°C Escherichia coli
1.11.1.21 210000
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase activity, mutant enzyme W105F, at pH 6.0 and 25°C Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.21 heme
-
 Escherichia coli

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.11.1.21 0.008
-
 mutant enzyme W105L, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity Escherichia coli azide
1.11.1.21 0.008
-
 wild type enzyme, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity Escherichia coli azide
1.11.1.21 0.01
-
 mutant enzyme R102K, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity Escherichia coli azide
1.11.1.21 0.012
-
 mutant enzyme W105F, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity Escherichia coli cyanide
1.11.1.21 0.04
-
 mutant enzyme H106C, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity Escherichia coli cyanide
1.11.1.21 0.055
-
 mutant enzyme R102L, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity Escherichia coli cyanide
1.11.1.21 0.065
-
 mutant enzyme W105L, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity Escherichia coli cyanide
1.11.1.21 0.075
-
 mutant enzyme R102C, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity Escherichia coli cyanide
1.11.1.21 0.09
-
 wild type enzyme, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity Escherichia coli cyanide
1.11.1.21 0.095
-
 mutant enzyme R102L, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity Escherichia coli azide
1.11.1.21 0.18
-
 mutant enzyme R102C, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity Escherichia coli azide
1.11.1.21 0.19
-
 mutant enzyme H106C, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity Escherichia coli azide
1.11.1.21 0.21
-
 mutant enzyme H106Y, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity Escherichia coli cyanide
1.11.1.21 0.23
-
 mutant enzyme H106Y, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity Escherichia coli azide
1.11.1.21 0.25
-
 mutant enzyme W105F, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity Escherichia coli azide
1.11.1.21 0.39
-
 mutant enzyme R102K, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity Escherichia coli cyanide