Literature summary extracted from
Cho, K.H.; Crane, B.R.; Park, S.
An insight into the interaction mode between CheB and chemoreceptor from two crystal structures of CheB methylesterase catalytic domain (2011), Biochem. Biophys. Res. Commun., 411, 69-75.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.1.1.61 |
expression of the N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) |
Thermotoga maritima |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
3.1.1.61 |
CheB methylesterase domain, X-ray diffraction structure determination and analysis at 2.2 A resolution |
Thermotoga maritima |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
3.1.1.61 |
CheB + H2O |
Thermotoga maritima |
substrate Cheb is a chemoreceptor |
? |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.1.1.61 |
Thermotoga maritima |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.1.1.61 |
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration |
Thermotoga maritima |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.1.1.61 |
CheB + H2O |
substrate Cheb is a chemoreceptor |
Thermotoga maritima |
? |
- |
? |
|
3.1.1.61 |
additional information |
surfaces with differing electrostatic properties may reflect CheB regions that mediate protein-protein interaction, computational docking, overview |
Thermotoga maritima |
? |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.1.1.61 |
More |
the Thermotoga maritima CheB methylesterase domain has identical topology of a modified doubly-wound alpha/beta fold, but the CheB demethylation consensus sites of the chemoreceptors, the CheB substrate, are not uniquely conserved |
Thermotoga maritima |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.1.1.61 |
CheB methylesterase |
- |
Thermotoga maritima |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
3.1.1.61 |
additional information |
interaction mode between CheB and chemoreceptors, overview |
Thermotoga maritima |