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Literature summary extracted from

  • Slavens, K.D.; Brown, T.R.; Barakat, K.A.; Cundari, T.R.; Anderson, M.E.
    Valine 44 and valine 45 of human glutathione synthetase are key for subunit stability and negative cooperativity (2011), Biochem. Biophys. Res. Commun., 410, 597-601.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
6.3.2.3 V44A decrease in melting temperature, slight decrease in activity Homo sapiens
6.3.2.3 V44A/V45A decrease in melting temperature, initial activity similar to wild-type Homo sapiens
6.3.2.3 V44W decrease in melting temperature, 16% decrease in activity Homo sapiens
6.3.2.3 V45A decrease in melting temperature, slight decrease in activity Homo sapiens
6.3.2.3 V45W decrease in melting temperature, 30% decrease in activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.3 0.32
-
 gamma-L-Glu-aminobutanoate mutant V45W, pH 8.2, 37°C Homo sapiens
6.3.2.3 0.95
-
 gamma-L-Glu-aminobutanoate mutant V44W, pH 8.2, 37°C Homo sapiens
6.3.2.3 1.42
-
 gamma-L-Glu-aminobutanoate mutant V44A/V45A, pH 8.2, 37°C Homo sapiens
6.3.2.3 1.42
-
 gamma-L-Glu-aminobutanoate wild-type, pH 8.2, 37°C Homo sapiens
6.3.2.3 1.54
-
 gamma-L-Glu-aminobutanoate mutant V45A, pH 8.2, 37°C Homo sapiens
6.3.2.3 1.6
-
 gamma-L-Glu-aminobutanoate mutant V44A, pH 8.2, 37°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.3 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.3 ATP + gamma-L-Glu-aminobutanoate + Gly
-
 Homo sapiens ADP + phosphate + ophthalmic acid
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.2.3 41.3
-
 melting temperature, mutant V45W Homo sapiens
6.3.2.3 42.8
-
 melting temperature, mutant V44A/V45A Homo sapiens
6.3.2.3 47.8
-
 melting temperature, mutant V45A Homo sapiens
6.3.2.3 51.1
-
 melting temperature, mutant V44A Homo sapiens
6.3.2.3 57
-
 melting temperature, mutant V44W Homo sapiens
6.3.2.3 60.4
-
 melting temperature, wild-type Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.2.3 13.6
-
 gamma-L-Glu-aminobutanoate mutant V45W, pH 8.2, 37°C Homo sapiens
6.3.2.3 16.4
-
 gamma-L-Glu-aminobutanoate mutant V44W, pH 8.2, 37°C Homo sapiens
6.3.2.3 17.4
-
 gamma-L-Glu-aminobutanoate mutant V45A, pH 8.2, 37°C Homo sapiens
6.3.2.3 18.9
-
 gamma-L-Glu-aminobutanoate mutant V44A, pH 8.2, 37°C Homo sapiens
6.3.2.3 19.5
-
 gamma-L-Glu-aminobutanoate wild-type, pH 8.2, 37°C Homo sapiens
6.3.2.3 21.7
-
 gamma-L-Glu-aminobutanoate mutant V44A/V45A, pH 8.2, 37°C Homo sapiens