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Literature summary extracted from

  • Senoura, T.; Ito, S.; Taguchi, H.; Higa, M.; Hamada, S.; Matsui, H.; Ozawa, T.; Jin, S.; Watanabe, J.; Wasaki, J.; Ito, S.
    New microbial mannan catabolic pathway that involves a novel mannosylglucose phosphorylase (2011), Biochem. Biophys. Res. Commun., 408, 701-716.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.281 expression in Escherichia coli Bacteroides fragilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.281 2
-
 phosphate pH 7.5, 30°C Bacteroides fragilis
2.4.1.281 8.3
-
 4-O-beta-D-mannopyranosyl-D-glucopyranose pH 7.5, 30°C Bacteroides fragilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.281 cytosol
-
 Bacteroides fragilis 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.281 43890
-
 calculated from amino acid sequence Bacteroides fragilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.281 4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate Bacteroides fragilis phosphorolysis reaction D-glucose + alpha-D-mannose 1-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.281 Bacteroides fragilis Q5LH68
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.281 28.5
-
 pH 7.5, 30°C Bacteroides fragilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.281 4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate phosphorolysis reaction Bacteroides fragilis D-glucose + alpha-D-mannose 1-phosphate
-
 r
2.4.1.281 additional information no substrates are 4-O-beta-D-xylopyranosyl-D-xylose, sophorose, sucrose, laminaribiose, cellobiose, lactose, epilactose, 4-O-beta-D-mannopyranosyl-D-mannose, gentiobiose, kojibiose, nigerose, maltose, isomaltose. Bacteroides fragilis ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.281 50
-
-
 Bacteroides fragilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.281 24.8
-
 phosphate pH 7.5, 30°C Bacteroides fragilis
2.4.1.281 42.2
-
 4-O-beta-D-mannopyranosyl-D-glucopyranose pH 7.5, 30°C Bacteroides fragilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.281 7
-
-
 Bacteroides fragilis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.281 5.1
-
 4-O-beta-D-mannopyranosyl-D-glucopyranose pH 7.5, 30°C Bacteroides fragilis
2.4.1.281 12.4
-
 phosphate pH 7.5, 30°C Bacteroides fragilis