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Literature summary extracted from

  • Dinescu, A.; Brown, T.R.; Barelier, S.; Cundari, T.R.; Anderson, M.E.
    The role of the glycine triad in human glutathione synthetase (2010), Biochem. Biophys. Res. Commun., 400, 511-516.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
6.3.2.3 G369V mutation in G-loop glycine triad, about 0.7% of wild-type activity. Mutation decreases ligand binding and prevent active site closure and protection Homo sapiens
6.3.2.3 G370V mutation in G-loop glycine triad, about 0.3% of wild-type activity. Mutation decreases ligand binding and prevent active site closure and protection Homo sapiens
6.3.2.3 G371V mutation in G-loop glycine triad, about 13% of wild-type activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.3 0.012
-
 ATP wild-type, 37°C, pH not specified in the publication Homo sapiens
6.3.2.3 0.1
-
 gamma-L-Glu-aminobutanoate mutant G371V, 37°C, pH not specified in the publication Homo sapiens
6.3.2.3 0.12
-
 ATP mutant G371V, 37°C, pH not specified in the publication Homo sapiens
6.3.2.3 0.44
-
 ATP mutant G369V, 37°C, pH not specified in the publication Homo sapiens
6.3.2.3 0.53
-
 Gly wild-type, 37°C, pH not specified in the publication Homo sapiens
6.3.2.3 0.89
-
 gamma-L-Glu-aminobutanoate mutant G369V, 37°C, pH not specified in the publication Homo sapiens
6.3.2.3 1.26
-
 gamma-L-Glu-aminobutanoate wild-type, 37°C, pH not specified in the publication Homo sapiens
6.3.2.3 2.52
-
 Gly mutant G371V, 37°C, pH not specified in the publication Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.3 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.3 ATP + gamma-L-Glu-aminobutanoate + Gly
-
 Homo sapiens ADP + phosphate + ophthalmic acid
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.2.3 0.052
-
 Gly mutant G370V, 37°C, pH not specified in the publication Homo sapiens
6.3.2.3 0.133
-
 Gly mutant G369V, 37°C, pH not specified in the publication Homo sapiens
6.3.2.3 2.36
-
 Gly mutant G371V, 37°C, pH not specified in the publication Homo sapiens
6.3.2.3 18.8
-
 Gly wild-type, 37°C, pH not specified in the publication Homo sapiens