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Literature summary extracted from

  • Xu, X.L.; Chen, S.; Grant, G.A.
    Kinetic, mutagenic, and structural homology analysis of L-serine dehydratase from Legionella pneumophila (2011), Arch. Biochem. Biophys., 515, 28-36.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.3.1.17 analysis development of a simple, direct, and continuous assay based on the absorbance of pyruvate, the product of the dehydratase reaction Legionella pneumophila

Protein Variants

EC Number Protein Variants Comment Organism
4.3.1.17 C183A 135% of wild-type activity. Enzyme retains the positive cooperativity seen in the native enzyme Legionella pneumophila
4.3.1.17 C221A 70% of wild-type activity. Enzyme retains the positive cooperativity seen in the native enzyme Legionella pneumophila
4.3.1.17 C226A 100% of wild-type activity. Enzyme retains the positive cooperativity seen in the native enzyme Legionella pneumophila
4.3.1.17 C304A 10% of wild-type activity. Mutant displays altered kinetics with a higher Km and a Hill coefficient indicating negative homotropic cooperativity Legionella pneumophila
4.3.1.17 C343A inactive, mutants lacks the charge transfer absorbance at 400 nm that is indicative of the 4Fe-4S center Legionella pneumophila
4.3.1.17 C385A inactive, mutants lacks the charge transfer absorbance at 400 nm that is indicative of the 4Fe-4S center Legionella pneumophila
4.3.1.17 C396A inactive, mutants lacks the charge transfer absorbance at 400 nm that is indicative of the 4Fe-4S center Legionella pneumophila
4.3.1.17 C458A 44% of wild-type activity. Enzyme retains the positive cooperativity seen in the native enzyme Legionella pneumophila

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.17 ammonia product inhibition, noncompetitive Legionella pneumophila
4.3.1.17 D-serine competitive Legionella pneumophila
4.3.1.17 L-cysteine competitive Legionella pneumophila
4.3.1.17 pyruvate product inhibition, competitive Legionella pneumophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.17 2
-
 L-Ser wild-type, pH 7.0, temperature not specified in the publication Legionella pneumophila
4.3.1.17 2.2
-
 L-Ser mutant C183A, pH 7.0, temperature not specified in the publication Legionella pneumophila
4.3.1.17 2.7
-
 L-Ser mutant C226A, pH 7.0, temperature not specified in the publication Legionella pneumophila
4.3.1.17 3
-
 L-Ser mutant C221A, pH 7.0, temperature not specified in the publication Legionella pneumophila
4.3.1.17 6.9
-
 L-Ser mutant C458A, pH 7.0, temperature not specified in the publication Legionella pneumophila
4.3.1.17 17.8
-
 L-Ser mutant C304A, pH 7.0, temperature not specified in the publication Legionella pneumophila

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.17 Legionella pneumophila Q5ZXE1 type 2 enzyme
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.17 L-Ser
-
 Legionella pneumophila pyruvate + NH3
-
 ir
4.3.1.17 L-Thr Km -value is approximately 150 times that for L-serine. At an L-threonine concentration of 300 mM, the enzyme activity is approximately 5% of the activity for L-serine at its Km of 2 mM Legionella pneumophila 2-oxobutanoate + NH3
-
 ?
4.3.1.17 additional information enzyme shows homotopic cooperativity, Hill coefficient of wild-type is 1.7 Legionella pneumophila ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.3.1.17 323
-
 L-Ser mutant C304A, pH 7.0, temperature not specified in the publication Legionella pneumophila
4.3.1.17 330
-
 L-Ser wild-type, pH 7.0, temperature not specified in the publication Legionella pneumophila
4.3.1.17 359
-
 L-Ser mutant C221A, pH 7.0, temperature not specified in the publication Legionella pneumophila
4.3.1.17 467
-
 L-Ser mutant C226A, pH 7.0, temperature not specified in the publication Legionella pneumophila
4.3.1.17 506
-
 L-Ser mutant C183A, pH 7.0, temperature not specified in the publication Legionella pneumophila
4.3.1.17 512
-
 L-Ser mutant C458A, pH 7.0, temperature not specified in the publication Legionella pneumophila

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.17 6 9 braod Legionella pneumophila
4.3.1.17 7
-
 assay at Legionella pneumophila

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.3.1.17 0.1
-
 L-cysteine pH 7.0, temperature not specified in the publication Legionella pneumophila
4.3.1.17 10
-
 D-serine pH 7.0, temperature not specified in the publication Legionella pneumophila

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.3.1.17 18
-
 L-Ser mutant C304A, pH 7.0, temperature not specified in the publication Legionella pneumophila
4.3.1.17 74
-
 L-Ser mutant C458A, pH 7.0, temperature not specified in the publication Legionella pneumophila
4.3.1.17 120
-
 L-Ser mutant C221A, pH 7.0, temperature not specified in the publication Legionella pneumophila
4.3.1.17 170
-
 L-Ser mutant C226A, pH 7.0, temperature not specified in the publication Legionella pneumophila
4.3.1.17 170
-
 L-Ser wild-type, pH 7.0, temperature not specified in the publication Legionella pneumophila
4.3.1.17 230
-
 L-Ser mutant C183A, pH 7.0, temperature not specified in the publication Legionella pneumophila