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Literature summary extracted from
- Properties of tryptophan indole-lyase from a piezophilic bacterium, Photobacterium profundum SS9 (2011), Arch. Biochem. Biophys., 506, 35-41.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.1.99.1 | - |
Escherichia coli |
| 4.1.99.1 | expressed in Escherichia coli BL21(DE3) tn5:tnaA | Photobacterium profundum |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 4.1.99.1 | activity decreases at pressures above 50 MPa, and by 100 MPa is less than 10% of the activity at 1 bar, initial increase in activity with pressure, reaching a maximum of about 140% at 30 MPa. | Escherichia coli |
| 4.1.99.1 | greater than 90% activity remaining at 100 MPa, initial increase in activity with pressure, reaching a maximum of about 140% at 60 MPa. | Photobacterium profundum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.99.1 | Escherichia coli | - |
- |
- |
| 4.1.99.1 | Photobacterium profundum | Q6LP64 | - |
- |
| 4.1.99.1 | Photobacterium profundum SS9 | Q6LP64 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.99.1 | hydrophobic interaction chromatography (phenyl-Sepharose) | Photobacterium profundum |
| 4.1.99.1 | hydrophobic interaction chromatography (Sepharose CL-aB) | Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.1.99.1 | 10 | - |
substrate S-(o-nitrophenyl)-L-cysteine, pH 8.0, 25°C | Photobacterium profundum |
| 4.1.99.1 | 34 | - |
substrate S-(o-nitrophenyl)-L-cysteine, pH 8.0, 25°C | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.99.1 | beta-chloro-L-Ala + H2O | - |
Escherichia coli | ? | - |
? |  |
| 4.1.99.1 | beta-chloro-L-Ala + H2O | - |
Photobacterium profundum | ? | - |
? | |
| 4.1.99.1 | beta-chloro-L-Ala + H2O | - |
Photobacterium profundum SS9 | ? | - |
? | |
| 4.1.99.1 | L-tryptophan + H2O | - |
Escherichia coli | indole + pyruvate + NH3 | - |
r | |
| 4.1.99.1 | L-tryptophan + H2O | - |
Photobacterium profundum | indole + pyruvate + NH3 | - |
r | |
| 4.1.99.1 | L-tryptophan + H2O | - |
Photobacterium profundum SS9 | indole + pyruvate + NH3 | - |
r | |
| 4.1.99.1 | S-(2-nitrophenyl)-L-cysteine + H2O | - |
Escherichia coli | 2-nitrobenzenethiolate + pyruvate + NH3 | - |
? | |
| 4.1.99.1 | S-(2-nitrophenyl)-L-cysteine + H2O | - |
Photobacterium profundum | 2-nitrobenzenethiolate + pyruvate + NH3 | - |
? | |
| 4.1.99.1 | S-(2-nitrophenyl)-L-cysteine + H2O | - |
Photobacterium profundum SS9 | 2-nitrobenzenethiolate + pyruvate + NH3 | - |
? | |
| 4.1.99.1 | S-benzyl-L-Cys + H2O | - |
Escherichia coli | phenylmethanethiol + pyruvate + NH4+ | - |
? | |
| 4.1.99.1 | S-benzyl-L-Cys + H2O | - |
Photobacterium profundum | phenylmethanethiol + pyruvate + NH4+ | - |
? | |
| 4.1.99.1 | S-benzyl-L-Cys + H2O | - |
Photobacterium profundum SS9 | phenylmethanethiol + pyruvate + NH4+ | - |
? | |
| 4.1.99.1 | S-ethyl-L-Cys + H2O | - |
Escherichia coli | ethanethiol + pyruvate + NH4+ | - |
? | |
| 4.1.99.1 | S-ethyl-L-Cys + H2O | - |
Photobacterium profundum | ethanethiol + pyruvate + NH4+ | - |
? | |
| 4.1.99.1 | S-ethyl-L-Cys + H2O | - |
Photobacterium profundum SS9 | ethanethiol + pyruvate + NH4+ | - |
? | |
| 4.1.99.1 | S-methyl-L-Cys + H2O | - |
Escherichia coli | methanethiol + pyruvate + NH4+ | - |
? | |
| 4.1.99.1 | S-methyl-L-Cys + H2O | - |
Photobacterium profundum | methanethiol + pyruvate + NH4+ | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.1.99.1 | tryptophan indole-lyase | - |
Escherichia coli |
| 4.1.99.1 | tryptophan indole-lyase | - |
Photobacterium profundum |
| 4.1.99.1 | tryptophanase | - |
Escherichia coli |
| 4.1.99.1 | tryptophanase | - |
Photobacterium profundum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.99.1 | additional information | - |
above 50°C, with S-(2-nitrophenyl)-L-cysteine | Escherichia coli |
| 4.1.99.1 | 30 | - |
with S-(2q-nitrophenyl)-L-cysteine | Photobacterium profundum |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.99.1 | additional information | - |
about 80% activity at 50°C after 2 hours, about 20% activity after incubation at 0°C for 3 hours | Escherichia coli |
| 4.1.99.1 | additional information | - |
rapid loss of activity at 50°C, no change in activity on incubation at 0°C for more than 3 hours | Photobacterium profundum |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.99.1 | 0.2 | - |
S-methyl-L-Cys | pH 8.0, 25°C | Photobacterium profundum | |
| 4.1.99.1 | 0.4 | - |
S-ethyl-L-Cys | pH 8.0, 25°C | Photobacterium profundum | |
| 4.1.99.1 | 1.1 | - |
S-benzyl-L-Cys | pH 8.0, 25°C | Photobacterium profundum | |
| 4.1.99.1 | 2.5 | - |
L-tryptophan | pH 8.0, 25°C | Photobacterium profundum | |
| 4.1.99.1 | 3 | - |
beta-chloro-L-Ala | pH 8.0, 25°C | Photobacterium profundum | |
| 4.1.99.1 | 5 | - |
S-methyl-L-Cys | pH 8.0, 25°C | Escherichia coli | |
| 4.1.99.1 | 5.2 | - |
S-benzyl-L-Cys | pH 8.0, 25°C | Escherichia coli | |
| 4.1.99.1 | 6 | - |
S-ethyl-L-Cys | pH 8.0, 25°C | Escherichia coli | |
| 4.1.99.1 | 6.8 | - |
L-tryptophan | pH 8.0, 25°C | Escherichia coli | |
| 4.1.99.1 | 12.4 | - |
beta-chloro-L-Ala | pH 8.0, 25°C | Escherichia coli | |
| 4.1.99.1 | 13.9 | - |
S-(2-nitrophenyl)-L-cysteine | pH 8.0, 25°C | Photobacterium profundum | |
| 4.1.99.1 | 44 | - |
S-(2-nitrophenyl)-L-cysteine | pH 8.0, 25°C | Escherichia coli | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.99.1 | pyridoxal 5'-phosphate | - |
Escherichia coli | |
| 4.1.99.1 | pyridoxal 5'-phosphate | - |
Photobacterium profundum | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.99.1 | 0.06 | - |
S-methyl-L-Cys | pH 8.0, 25°C | Photobacterium profundum | |
| 4.1.99.1 | 0.28 | - |
S-ethyl-L-Cys | pH 8.0, 25°C | Photobacterium profundum | |
| 4.1.99.1 | 0.33 | - |
S-methyl-L-Cys | pH 8.0, 25°C | Escherichia coli | |
| 4.1.99.1 | 0.77 | - |
beta-chloro-L-Ala | pH 8.0, 25°C | Photobacterium profundum | |
| 4.1.99.1 | 2.4 | - |
beta-chloro-L-Ala | pH 8.0, 25°C | Escherichia coli | |
| 4.1.99.1 | 7.6 | - |
L-tryptophan | pH 8.0, 25°C | Photobacterium profundum | |
| 4.1.99.1 | 9 | - |
S-ethyl-L-Cys | pH 8.0, 25°C | Escherichia coli | |
| 4.1.99.1 | 11 | - |
S-benzyl-L-Cys | pH 8.0, 25°C | Photobacterium profundum | |
| 4.1.99.1 | 29 | - |
S-(2-nitrophenyl)-L-cysteine | pH 8.0, 25°C | Photobacterium profundum | |
| 4.1.99.1 | 30 | - |
L-tryptophan | pH 8.0, 25°C | Escherichia coli | |
| 4.1.99.1 | 81 | - |
S-benzyl-L-Cys | pH 8.0, 25°C | Escherichia coli | |
| 4.1.99.1 | 510 | - |
S-(2-nitrophenyl)-L-cysteine | pH 8.0, 25°C | Escherichia coli | |
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