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Literature summary extracted from
- Engineering Bacillus subtilis for isobutanol production by heterologous Ehrlich pathway construction and the biosynthetic 2-ketoisovalerate precursor pathway overexpression (2011), Appl. Microbiol. Biotechnol., 91, 577-589.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.1.9 | gene ilvD, co-expression with other enzymes of the branched amino acid biosynthesis pathway in Bacillus subtilis strain 168 for production of isobutanol via the Ehrlich pathway, overview. Bacillus subtilis is engineered as the cell factory for isobutanol production due to its high tolerance to isobutanol. Subcloning in Escherichia coli strain JM109 | Corynebacterium glutamicum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.9 | 2,3-dihydroxy-3-methylbutanoate | Corynebacterium glutamicum | - |
3-methyl-2-oxobutanoate + H2O | i.e. 2-ketoisovalerate | ? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.9 | Corynebacterium glutamicum | Q8NQZ9 | gene ilvD | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.9 | 2,3-dihydroxy-3-methylbutanoate | - |
Corynebacterium glutamicum | 3-methyl-2-oxobutanoate + H2O | i.e. 2-ketoisovalerate | ? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.1.9 | IlvD | - |
Corynebacterium glutamicum |
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Release 2023.1 (January 2023)
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