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Literature summary extracted from

  • Sakamoto, T.; Tanaka, H.; Nishimura, Y.; Ishimaru, M.; Kasai, N.
    Characterization of an exo-beta-1,3-D-galactanase from Sphingomonas sp. 24T and its application to structural analysis of larch wood arabinogalactan (2011), Appl. Microbiol. Biotechnol., 90, 1701-1710.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.145 additional information addition of galactose at a concentration of 2 mM to the reaction mixture does not affect the enzyme activity Sphingomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.145 48000
-
 x * 48000, SDS-PAGE Sphingomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.145 Sphingomonas sp.
-
-
-
3.2.1.145 Sphingomonas sp. 24T
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.145 ammonium sulfate precipitation, DEAE-Toyopearl 650-M column chromatography and Resource PHE column chromatography Sphingomonas sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.145 0.88
-
 culture filtrate, using beta-(1-3)-D-galactan as substrate, in 20 mM Na phosphate buffer (pH 6.0) at 37°C Sphingomonas sp.
3.2.1.145 1.7
-
 after 1.9fold purification, using beta-(1-3)-D-galactan as substrate, in 20 mM Na phosphate buffer (pH 6.0) at 37°C Sphingomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.145 beta-(1,3)-D-galactooligosaccharides + H2O
-
 Sphingomonas sp. D-galactose + ?
-
 ?
3.2.1.145 beta-(1,3)-D-galactooligosaccharides + H2O
-
 Sphingomonas sp. 24T D-galactose + ?
-
 ?
3.2.1.145 beta-(1,3)-galactobiose + H2O
-
 Sphingomonas sp. D-galactose
-
 ?
3.2.1.145 beta-(1,3)-galactobiose + H2O
-
 Sphingomonas sp. 24T D-galactose
-
 ?
3.2.1.145 beta-(1-3)-D-galactan + H2O the enzyme releases approximately 60% of the total D-galactose in the substrate Sphingomonas sp. beta-(1-3)-D-galactan + D-galactose
-
 ?
3.2.1.145 beta-(1-3)-D-galactan + H2O the enzyme releases approximately 60% of the total D-galactose in the substrate Sphingomonas sp. 24T beta-(1-3)-D-galactan + D-galactose
-
 ?
3.2.1.145 beta-1,3-galactotriose + H2O
-
 Sphingomonas sp. D-galactose + beta-(1,3)-galactobiose
-
 ?
3.2.1.145 beta-1,3-galactotriose + H2O
-
 Sphingomonas sp. 24T D-galactose + beta-(1,3)-galactobiose
-
 ?
3.2.1.145 gum arabic + H2O weak substrate Sphingomonas sp. ?
-
 ?
3.2.1.145 larch wood arabinogalactan + H2O the enzyme bypasses the branching points of beta-1,3-galactan backbones in larch wood arabinogalactan Sphingomonas sp. D-galactose + beta-(1->6)-D-galactobiose + beta-(1->6)-D-galactotriose + O-alpha-L-arabinofuranosyl-(1->3)-O-beta-D-galactopyranosyl-(1->6)-O-beta-D-galactopyranosyl-(1->6)-D-galactopyranose + O-beta-D-galactopyranosyl-(1->6)-[O-alpha-L-arabinofuranosyl-(1->3)-]-O-beta-D-galactopyranosyl-(1->6)-D-galactopyranose + ?
-
 ?
3.2.1.145 additional information no activity with lupin galactan, carob galactomannan, sugar beet L-arabinan, sugar beet debranched arabinan, wheat arabinoxylan, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-xylopyranoside, 4-nitrophenyl alpha-L-arabinopyranoside, and 4-nitrophenyl alpha-L-arabinofuranoside. The enzyme is not active on galactooligosaccharides with beta-1,4- or beta-1,6-linkages Sphingomonas sp. ?
-
 ?
3.2.1.145 additional information no activity with lupin galactan, carob galactomannan, sugar beet L-arabinan, sugar beet debranched arabinan, wheat arabinoxylan, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-xylopyranoside, 4-nitrophenyl alpha-L-arabinopyranoside, and 4-nitrophenyl alpha-L-arabinofuranoside. The enzyme is not active on galactooligosaccharides with beta-1,4- or beta-1,6-linkages Sphingomonas sp. 24T ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.145 ? x * 48000, SDS-PAGE Sphingomonas sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.145 Exo-1,3-Gal
-
 Sphingomonas sp.
3.2.1.145 exo-beta-1,3-D-galactanase
-
 Sphingomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.145 50
-
 the enzyme is most active around 50°C Sphingomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.145 40
-
 the enzyme is stable at temperatures up to 40°C Sphingomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.145 6 7 at 37°C Sphingomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.145 3 10 more than 80% of the initial activity remains after 16 h of incubation at pH values from 3.0 to 10.0 Sphingomonas sp.