EC Number | Cloned (Comment) | Organism |
---|---|---|
5.3.1.6 | expressed in Escherichia coli ER2566 cells | Acetivibrio thermocellus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
5.3.1.6 | C65A | the mutant shows no activity for D-psicose | Acetivibrio thermocellus |
5.3.1.6 | D8A | the mutant shows no activity for D-psicose | Acetivibrio thermocellus |
5.3.1.6 | H133A | the mutant shows 15% activity compared to the wild type enzyme | Acetivibrio thermocellus |
5.3.1.6 | H98A | the mutant shows 13% activity compared to the wild type enzyme | Acetivibrio thermocellus |
5.3.1.6 | H9A | the mutant shows 63% activity compared to the wild type enzyme | Acetivibrio thermocellus |
5.3.1.6 | N99A | the mutant shows 35% activity compared to the wild type enzyme | Acetivibrio thermocellus |
5.3.1.6 | R132A | the mutant exhibits an increase in D-psicose isomerization activity (116% activity compared to the wild type enzyme) | Acetivibrio thermocellus |
5.3.1.6 | R132D | the mutant shows increased catalytic efficiency toward D-psicose compared to the wild type enzyme | Acetivibrio thermocellus |
5.3.1.6 | R132E | the mutant shows increased catalytic efficiency toward D-psicose compared to the wild type enzyme | Acetivibrio thermocellus |
5.3.1.6 | R132E | the specific activity and catalytic efficiency (kcat/Km) of the R132E mutant for D-psicose are 1.4 and 1.5fold higher than those of the wild type enzyme, respectively | Acetivibrio thermocellus |
5.3.1.6 | R132I | the mutant shows decreased catalytic efficiency toward D-psicose compared to the wild type enzyme | Acetivibrio thermocellus |
5.3.1.6 | R132K | the mutant shows decreased catalytic efficiency toward D-psicose compared to the wild type enzyme | Acetivibrio thermocellus |
5.3.1.6 | R132Q | the mutant shows decreased catalytic efficiency toward D-psicose compared to the wild type enzyme | Acetivibrio thermocellus |
5.3.1.6 | R136A | the mutant shows 15% activity compared to the wild type enzyme | Acetivibrio thermocellus |
5.3.1.6 | T135A | the mutant shows 91% activity compared to the wild type enzyme | Acetivibrio thermocellus |
5.3.1.6 | T67A | the mutant shows 46% activity compared to the wild type enzyme | Acetivibrio thermocellus |
5.3.1.6 | Y42A | the mutant shows no activity for D-psicose | Acetivibrio thermocellus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.6 | 43 | - |
D-psicose | mutant enzyme R132E, pH 7.5, 80°C | Acetivibrio thermocellus | |
5.3.1.6 | 46 | - |
D-psicose | mutant enzyme R132D, pH 7.5, 80°C | Acetivibrio thermocellus | |
5.3.1.6 | 53 | - |
D-psicose | mutant enzyme R132K, pH 7.5, 80°C | Acetivibrio thermocellus | |
5.3.1.6 | 53 | - |
D-psicose | wild type enzyme, pH 7.5, 80°C | Acetivibrio thermocellus | |
5.3.1.6 | 54 | - |
D-psicose | mutant enzyme R132Q, pH 7.5, 80°C | Acetivibrio thermocellus | |
5.3.1.6 | 56 | - |
D-psicose | mutant enzyme R132A, pH 7.5, 80°C | Acetivibrio thermocellus | |
5.3.1.6 | 78 | - |
D-psicose | mutant enzyme R132I, pH 7.5, 80°C | Acetivibrio thermocellus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.3.1.6 | Acetivibrio thermocellus | A3DIL8 | - |
- |
5.3.1.6 | Acetivibrio thermocellus ATCC 27405 | A3DIL8 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.3.1.6 | HisTrap HP column chromatography | Acetivibrio thermocellus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
5.3.1.6 | 0.259 | - |
mutant enzyme H98A, using D-psicose as substrate, pH 7.5, 80°C | Acetivibrio thermocellus |
5.3.1.6 | 0.299 | - |
mutant enzyme H133A, using D-psicose as substrate, pH 7.5, 80°C | Acetivibrio thermocellus |
5.3.1.6 | 0.299 | - |
mutant enzyme R136A, using D-psicose as substrate, pH 7.5, 80°C | Acetivibrio thermocellus |
5.3.1.6 | 0.698 | - |
mutant enzyme N99A, using D-psicose as substrate, pH 7.5, 80°C | Acetivibrio thermocellus |
5.3.1.6 | 0.917 | - |
mutant enzyme T67A, using D-psicose as substrate, pH 7.5, 80°C | Acetivibrio thermocellus |
5.3.1.6 | 1.256 | - |
mutant enzyme H9A, using D-psicose as substrate, pH 7.5, 80°C | Acetivibrio thermocellus |
5.3.1.6 | 1.815 | - |
mutant enzyme T135A, using D-psicose as substrate, pH 7.5, 80°C | Acetivibrio thermocellus |
5.3.1.6 | 1.994 | - |
wild type enzyme, using D-psicose as substrate, pH 7.5, 80°C | Acetivibrio thermocellus |
5.3.1.6 | 2.313 | - |
mutant enzyme R132A, using D-psicose as substrate, pH 7.5, 80°C | Acetivibrio thermocellus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.1.6 | D-psicose | the conversion yield of D-psicose to D-allose is 32% for the R132E mutant enzyme and 25% for the wild type enzyme after 80 min | Acetivibrio thermocellus | D-allose | - |
r | |
5.3.1.6 | D-psicose | the conversion yield of D-psicose to D-allose is 32% for the R132E mutant enzyme and 25% for the wild type enzyme after 80 min | Acetivibrio thermocellus ATCC 27405 | D-allose | - |
r | |
5.3.1.6 | D-ribulose 5-phosphate | - |
Acetivibrio thermocellus | D-ribose 5-phosphate | - |
r | |
5.3.1.6 | D-ribulose 5-phosphate | - |
Acetivibrio thermocellus ATCC 27405 | D-ribose 5-phosphate | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.3.1.6 | CTRpiB | - |
Acetivibrio thermocellus |
5.3.1.6 | ribose-5-phosphate isomerase | - |
Acetivibrio thermocellus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.3.1.6 | 80 | - |
- |
Acetivibrio thermocellus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.3.1.6 | 60 | 90 | - |
Acetivibrio thermocellus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.3.1.6 | 60 | 75 | the half-lives of the wild type enzyme at 60°C, 65°C, 70°C, 75°C, and 80°C are 11, 7.0, 4.2, 1.5, and 0.6 h, respectively | Acetivibrio thermocellus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.6 | 1962 | - |
D-psicose | mutant enzyme R132K, pH 7.5, 80°C | Acetivibrio thermocellus | |
5.3.1.6 | 2211 | - |
D-psicose | mutant enzyme R132I, pH 7.5, 80°C | Acetivibrio thermocellus | |
5.3.1.6 | 2335 | - |
D-psicose | mutant enzyme R132Q, pH 7.5, 80°C | Acetivibrio thermocellus | |
5.3.1.6 | 2347 | - |
D-psicose | wild type enzyme, pH 7.5, 80°C | Acetivibrio thermocellus | |
5.3.1.6 | 2580 | - |
D-psicose | mutant enzyme R132A, pH 7.5, 80°C | Acetivibrio thermocellus | |
5.3.1.6 | 2674 | - |
D-psicose | mutant enzyme R132D, pH 7.5, 80°C | Acetivibrio thermocellus | |
5.3.1.6 | 2743 | - |
D-psicose | mutant enzyme R132E, pH 7.5, 80°C | Acetivibrio thermocellus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.3.1.6 | 7.5 | - |
- |
Acetivibrio thermocellus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
5.3.1.6 | 6 | 9 | - |
Acetivibrio thermocellus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.6 | 28 | - |
D-psicose | mutant enzyme R132I, pH 7.5, 80°C | Acetivibrio thermocellus | |
5.3.1.6 | 37 | - |
D-psicose | mutant enzyme R132K, pH 7.5, 80°C | Acetivibrio thermocellus | |
5.3.1.6 | 43 | - |
D-psicose | mutant enzyme R132Q, pH 7.5, 80°C | Acetivibrio thermocellus | |
5.3.1.6 | 44 | - |
D-psicose | wild type enzyme, pH 7.5, 80°C | Acetivibrio thermocellus | |
5.3.1.6 | 46 | - |
D-psicose | mutant enzyme R132A, pH 7.5, 80°C | Acetivibrio thermocellus | |
5.3.1.6 | 58 | - |
D-psicose | mutant enzyme R132D, pH 7.5, 80°C | Acetivibrio thermocellus | |
5.3.1.6 | 64 | - |
D-psicose | mutant enzyme R132E, pH 7.5, 80°C | Acetivibrio thermocellus |