Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Shao, W.; Xue, Y.; Wu, A.; Kataeva, I.; Pei, J.; Wu, H.; Wiegel, J.
    Characterization of a novel beta-xylosidase, XylC, from Thermoanaerobacterium saccharolyticum JW/SL-YS485 (2011), Appl. Environ. Microbiol., 77, 719-726.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.37 additional information with use of vector pHsh, expression of XylC increases approximately 4fold when a loop within the translational initiation region in the mRNA is removed by site-directed mutagenesis. The increased expression of xylCm is due to removal of a stem-loop structure without a change of the amino acid sequence of the heterologously expressed enzyme Thermoanaerobacterium saccharolyticum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.37 additional information EDTA is not inhibitory up to 10 mM Thermoanaerobacterium saccharolyticum
3.2.1.37 xylose not inhibitory up to 70 mM, 70% of its activity is retained in the presence of 200 mM xylose Thermoanaerobacterium saccharolyticum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.37 28
-
 4-nitrophenyl-beta-D-xylopyranoside pH 6.0, 65°C Thermoanaerobacterium saccharolyticum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.37 78000
-
 x * 78000, SDS-PAGE Thermoanaerobacterium saccharolyticum
3.2.1.37 86000
-
 x * 86000, SDS-PAGE Thermoanaerobacterium saccharolyticum
3.2.1.37 112000
-
 PAGE Thermoanaerobacterium saccharolyticum
3.2.1.37 122000
-
 gel filtration Thermoanaerobacterium saccharolyticum
3.2.1.37 165000
-
 PAGE Thermoanaerobacterium saccharolyticum
3.2.1.37 210000
-
 gel filtration Thermoanaerobacterium saccharolyticum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.37 Thermoanaerobacterium saccharolyticum
-
-
-
3.2.1.37 Thermoanaerobacterium saccharolyticum A2ICH1
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.37 glycoprotein 6% carbohydrate content Thermoanaerobacterium saccharolyticum
3.2.1.37 no glycoprotein
-
 Thermoanaerobacterium saccharolyticum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.37 45.8
-
 pH 6.0, 65°C Thermoanaerobacterium saccharolyticum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.37 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Thermoanaerobacterium saccharolyticum 4-nitrophenol + beta-D-xylose
-
 ?
3.2.1.37 additional information enzyme produces beta-xylose from beta-xylopyranosides while retaining the anomeric configuration of the xylose in the substrate. Enzyme shows transglycosylation activities in the presence of alcohols as acceptors. No substrate: 4-nitrophenyl-alpha-L-arabinofuranoside, 4-nitrophenyl-alpha-D-xylopyranoside, 4-nitrophenyl-alpha-D-glucopyranoside, and 4-nitrophenyl-beta-D-glucopyranoside, oat spelt xylan, birch wood xylan, or carboxymethyl cellulose Thermoanaerobacterium saccharolyticum ?
-
 ?
3.2.1.37 xylobiose + H2O
-
 Thermoanaerobacterium saccharolyticum 2 beta-D-xylose
-
 ?
3.2.1.37 xylotriose + 2 H2O
-
 Thermoanaerobacterium saccharolyticum 3 beta-D-xylose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.37 ? x * 78000, SDS-PAGE Thermoanaerobacterium saccharolyticum
3.2.1.37 ? x * 86000, SDS-PAGE Thermoanaerobacterium saccharolyticum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.37 XYLA
-
 Thermoanaerobacterium saccharolyticum
3.2.1.37 XylC
-
 Thermoanaerobacterium saccharolyticum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.37 65
-
-
 Thermoanaerobacterium saccharolyticum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.37 67
-
 half-life 1 h Thermoanaerobacterium saccharolyticum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.37 189.3
-
 4-nitrophenyl-beta-D-xylopyranoside pH 6.0, 65°C Thermoanaerobacterium saccharolyticum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.37 6
-
-
 Thermoanaerobacterium saccharolyticum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.37 6 8 stable within Thermoanaerobacterium saccharolyticum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.37 Thermoanaerobacterium saccharolyticum isoelectric focusing
-
 4.4
3.2.1.37 Thermoanaerobacterium saccharolyticum isoelectric focusing
-
 4.5