BRENDA - Enzyme Database

Preparation and properties of isocitrate lyase isoforms from the cotyledons of Glycine max L

Eprintsev, A.; Dyachenko, E.; Lykova, T.; Kuen, C.; Popov, V.; Appl. Biochem. Microbiol. 46, 93-98 (2010)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.016
-
isocitrate
pH 7.5, 25°C
Glycine max
4.1.3.1
0.05
-
isocitrate
pH 7.5, 25°C
Glycine max
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.1.3.1
glyoxysome
-
Glycine max
9514
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
66000
-
4 * 66000, SDS-PAGE, gel filtration
Glycine max
4.1.3.1
246000
-
gel filtration
Glycine max
4.1.3.1
272000
-
gel filtration
Glycine max
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Glycine max
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.1
ammonium sulfate frationation, gel filtration, ion exchange chromatography (DEAE-cellulose)
Glycine max
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.3.1
cotyledon
-
Glycine max
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
5.28
-
pH 7.5, 25°C
Glycine max
4.1.3.1
5.81
-
pH 7.5, 25°C
Glycine max
Storage Stability
EC Number
Storage Stability
Organism
4.1.3.1
-20°C, Tris-HCl-buffer, pH 7.5, 50% loss of activity after 3 days, 100% loss of activity after 6 days
Glycine max
4.1.3.1
-70°C, Tris-HCl-buffer, pH 7.5, 5% loss of activity after 1 day, 10% loss of activity for each following day
Glycine max
4.1.3.1
-70°C, Tris-HCl-buffer, pH 7.5, 80% activity by the tenth day of storage in the presence of 5 mM Mg2+ and 25% of activity when 5 mM Mn2+ are used for stabilization, the following storage conditions are optimal: low temperature (-70°C), 25% glycerol in the medium and the presence of 5 mM MgCl2
Glycine max
4.1.3.1
-70°C, Tris-HCl-buffer, pH 7.5, 85% activity by the tenth day of storage in the presence of 5 mM Mn2+ and 30% of activity when 5 mM Mg2+ are used for stabilization, the following storage conditions are optimal: low temperature (-70°C), 25% glycerol in the medium and the presence of 5 mM MnCl2
Glycine max
4.1.3.1
room temperature, Tris-HCl-buffer, pH 7.5, 80% loss of activity in 24 hours
Glycine max
4.1.3.1
storage in glycerol-containing medium (25% glycerol) results in a 30% higher activity than storage in the absence of glycerol
Glycine max
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
713812
Glycine max
glyoxylate + succinate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.1.3.1
homotetramer
4 * 66000, SDS-PAGE, gel filtration
Glycine max
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
6.5
-
-
Glycine max
4.1.3.1
8
-
-
Glycine max
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.016
-
isocitrate
pH 7.5, 25°C
Glycine max
4.1.3.1
0.05
-
isocitrate
pH 7.5, 25°C
Glycine max
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.1.3.1
glyoxysome
-
Glycine max
9514
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
66000
-
4 * 66000, SDS-PAGE, gel filtration
Glycine max
4.1.3.1
246000
-
gel filtration
Glycine max
4.1.3.1
272000
-
gel filtration
Glycine max
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
ammonium sulfate frationation, gel filtration, ion exchange chromatography (DEAE-cellulose)
Glycine max
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.3.1
cotyledon
-
Glycine max
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
5.28
-
pH 7.5, 25°C
Glycine max
4.1.3.1
5.81
-
pH 7.5, 25°C
Glycine max
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.1.3.1
-20°C, Tris-HCl-buffer, pH 7.5, 50% loss of activity after 3 days, 100% loss of activity after 6 days
Glycine max
4.1.3.1
-70°C, Tris-HCl-buffer, pH 7.5, 5% loss of activity after 1 day, 10% loss of activity for each following day
Glycine max
4.1.3.1
-70°C, Tris-HCl-buffer, pH 7.5, 80% activity by the tenth day of storage in the presence of 5 mM Mg2+ and 25% of activity when 5 mM Mn2+ are used for stabilization, the following storage conditions are optimal: low temperature (-70°C), 25% glycerol in the medium and the presence of 5 mM MgCl2
Glycine max
4.1.3.1
-70°C, Tris-HCl-buffer, pH 7.5, 85% activity by the tenth day of storage in the presence of 5 mM Mn2+ and 30% of activity when 5 mM Mg2+ are used for stabilization, the following storage conditions are optimal: low temperature (-70°C), 25% glycerol in the medium and the presence of 5 mM MnCl2
Glycine max
4.1.3.1
room temperature, Tris-HCl-buffer, pH 7.5, 80% loss of activity in 24 hours
Glycine max
4.1.3.1
storage in glycerol-containing medium (25% glycerol) results in a 30% higher activity than storage in the absence of glycerol
Glycine max
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
713812
Glycine max
glyoxylate + succinate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.3.1
homotetramer
4 * 66000, SDS-PAGE, gel filtration
Glycine max
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
6.5
-
-
Glycine max
4.1.3.1
8
-
-
Glycine max