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Literature summary extracted from

  • Kumar, K.; Bhargava, P.; Roy, U.
    In Vitro Refolding of Triosephosphate Isomerase from L. donovani (2011), Appl. Biochem. Biotechnol., 164, 1207-1214.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.1 in expression vector pET-23(a) with C-terminal His6 tag fused in frame, expressed in Escherichia coli BL21(DE3) cells Leishmania donovani

General Stability

EC Number General Stability Organism
5.3.1.1 bovine serum albunin assists in the refolding and regain of TIM enzyme activity by providing framework for structure formation Leishmania donovani

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.1 27600
-
 x * 27600, SDS-PAGE Leishmania donovani

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.1 Leishmania donovani Q0ZAG6
-
-
5.3.1.1 Leishmania donovani MHOM/IN/80/Dd8 Q0ZAG6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.1 Ni-NTA column chromatography Leishmania donovani

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.1 D-glyceraldehyde 3-phosphate
-
 Leishmania donovani dihydroxyacetone phosphate
-
 ?
5.3.1.1 D-glyceraldehyde 3-phosphate
-
 Leishmania donovani MHOM/IN/80/Dd8 dihydroxyacetone phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.1 ? x * 27600, SDS-PAGE Leishmania donovani

Synonyms

EC Number Synonyms Comment Organism
5.3.1.1 TIM
-
 Leishmania donovani
5.3.1.1 Triosephosphate isomerase
-
 Leishmania donovani