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Literature summary extracted from

  • Vohra, A.; Kaur, P.; Satyanarayana, T.
    Production, characteristics and applications of the cell-bound phytase of Pichia anomala (2011), Antonie van Leeuwenhoek, 99, 51-55.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.8 gene PPHY, DNA sequence, promoter and terminator region, and amino acid sequence determination and analysis Wickerhamomyces anomalus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.8 2,3-Butanedione
-
 Wickerhamomyces anomalus
3.1.3.8 guanidine hydrochloride
-
 Wickerhamomyces anomalus
3.1.3.8 additional information no inhibition by sodium azide, DTT, 2-mercaptoethanol, EDTA, toluene, glycerol, and PMSF Wickerhamomyces anomalus
3.1.3.8 phosphate
-
 Wickerhamomyces anomalus
3.1.3.8 Triton X-100
-
 Wickerhamomyces anomalus
3.1.3.8 Tween 20
-
 Wickerhamomyces anomalus
3.1.3.8 Tween 80
-
 Wickerhamomyces anomalus
3.1.3.8 Urea
-
 Wickerhamomyces anomalus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.8 0.2
-
 myo-inositol-1,2,3,4,5,6-hexakisphosphate pH 4.0, 60°C Wickerhamomyces anomalus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.8 cell bound
-
 Wickerhamomyces anomalus
-
-
3.1.3.8 cell wall about 78% of the enzyme is cell wall bound Wickerhamomyces anomalus 5618
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.8 additional information no requirement of metal ions for the activity Wickerhamomyces anomalus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.8 64000
-
 6 * 64000, SDS-PAGE Wickerhamomyces anomalus
3.1.3.8 384000
-
 about, native PAGE Wickerhamomyces anomalus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.8 additional information Wickerhamomyces anomalus the cell-bound phytase is able to effectively dephytinize wheat flour, wheat bran, rice flour, and soybean flour, or soymilk, to varied extents ?
-
 ?
3.1.3.8 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O Wickerhamomyces anomalus
-
 ? + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Wickerhamomyces anomalus D3HIF3 single copy gene PPHY
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8 the native enzyme is purified to near homogeneity by acetone precipitation and anion exchange chromatography Wickerhamomyces anomalus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.8 additional information optimization of fermentation for production of cell-bound phytase, overview Wickerhamomyces anomalus
-

Storage Stability

EC Number Storage Stability Organism
3.1.3.8 purified enzyme, shelf life is 6 months at 4°C, no loss in the activity on lyophilization Wickerhamomyces anomalus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 additional information the cell-bound phytase is able to effectively dephytinize wheat flour, wheat bran, rice flour, and soybean flour, or soymilk, to varied extents Wickerhamomyces anomalus ?
-
 ?
3.1.3.8 additional information the phytase exhibits broad substrate specificity since it hydrolyses 4-nitrophenyl phosphate, ATP, ADP, and glucose-6-phosphate besides phytic acid. The enzyme hydrolyzes insoluble calcium and magnesium phytates efficiently, but not iron phytate Wickerhamomyces anomalus ?
-
 ?
3.1.3.8 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
-
 Wickerhamomyces anomalus ? + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.8 homohexamer 6 * 64000, SDS-PAGE Wickerhamomyces anomalus

Synonyms

EC Number Synonyms Comment Organism
3.1.3.8 cell-bound phytase
-
 Wickerhamomyces anomalus
3.1.3.8 histidine acid phosphatase
-
 Wickerhamomyces anomalus
3.1.3.8 More PPHY is a phytase belonging to histidine acid phosphatases Wickerhamomyces anomalus
3.1.3.8 PPHY
-
 Wickerhamomyces anomalus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 60
-
 purified phytase Wickerhamomyces anomalus
3.1.3.8 75
-
 cell bound phytase Wickerhamomyces anomalus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 additional information
-
 the cell-bound phytase exhibits better thermostability than the pure enzyme Wickerhamomyces anomalus
3.1.3.8 60
-
 purified enzyme, half-life is 7 days Wickerhamomyces anomalus
3.1.3.8 70
-
 purified enzyme, half-life is 48 h Wickerhamomyces anomalus
3.1.3.8 80
-
 purified enzyme, half-life is 5 min Wickerhamomyces anomalus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 4
-
-
 Wickerhamomyces anomalus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.3.8 Wickerhamomyces anomalus isoelectric focusing
-
 4.5