EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.277 | the recombinant DesVII protein is overproduced in Escherichia coli as a C-terminal His6-tagged fusion protein. The DesVIII protein is produced in Escherichia coli as a fusion with maltose-binding protein at its N-terminus. The fusion protein is cleaved by treatment with protease and untagged DesVIII protein is used in the assays together with DesVII | Streptomyces venezuelae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.277 | TDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + 10-deoxymethynolide | Streptomyces venezuelae | - |
dTDP + 10-deoxymethymycin | - |
? | |
2.4.1.277 | TDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + narbonolide | Streptomyces venezuelae | - |
dTDP + narbomycin | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.277 | Streptomyces venezuelae | Q9ZGH7 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.277 | - |
Streptomyces venezuelae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.277 | TDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + 10-deoxymethynolide | - |
Streptomyces venezuelae | dTDP + 10-deoxymethymycin | - |
? | |
2.4.1.277 | TDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + 10-deoxymethynolide | DesVII/DesVIII exhibits a stringent 6-deoxysugar requirement but an otherwise relaxed substrate specificity toward both TDP-sugar donor and aglycone acceptor | Streptomyces venezuelae | dTDP + 10-deoxymethymycin | - |
? | |
2.4.1.277 | TDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + narbonolide | - |
Streptomyces venezuelae | dTDP + narbomycin | - |
? | |
2.4.1.277 | TDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + narbonolide | DesVII/DesVIII exhibits a stringent 6-deoxysugar requirement but an otherwise relaxed substrate specificity toward both TDP-sugar donor and aglycone acceptor | Streptomyces venezuelae | dTDP + narbomycin | - |
? |