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Literature summary extracted from

  • Ariyawutthiphan, O.; Ose, T.; Tsuda, M.; Gao, Y.; Yao, M.; Minami, A.; Oikawa, H.; Tanaka, I.
    Crystallization and preliminary X-ray crystallographic study of a methyltransferase involved in 2-methylisoborneol biosynthesis in Streptomyces lasaliensis (2011), Acta Crystallogr. Sect. F, 67, 417-420.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.255 expression of His6-tagged enzyme in Escherichia coli strain BL21 (DE3) Streptomyces lasalocidi
2.1.1.255 overproduced in Escherichia coli strain BL21 Streptomyces lasalocidi

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.255 purified recombinant His6-tagged apo-GPPMT containing one molecule in the asymmetric unit, or in ternary complex with geranyl diphosphate and sinefungin, hanging drop method, mixing of protein solution containing 5 mg/ml in 50 mM sodium phosphate, pH 7.5, 300 mM NaCl and 10% glycerol, with S-adenosyl-L-methionine at a protein:S-adenosyl-L-methionine molar ratio of 1:7, 4°C, and further with reservoir solution containing 50 mM sodium cacodylate, pH 6.5, 1.4 M lithium sulfate, 30 mM MgCl2, and 1 mM spermine for GPPMT-GPP-SFG form I, or 50 mM sodium cacodylate, pH 6.7, 1.3 M lithium sulfate, 30 mM MgCl2, and 1 mM spermine for GPPMT-GPP-SFG form II, method screening, X-ray diffraction structure determination and analysis at 1.7-3.5 A resolution, molecular replacement method Streptomyces lasalocidi
2.1.1.255 sitting-drop vapour-diffusion method Streptomyces lasalocidi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate Streptomyces lasalocidi methylation of geranyl diphosphate before the cyclization reaction S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.255 Streptomyces lasalocidi D3KYU3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.255
-
 Streptomyces lasalocidi
2.1.1.255 recombinant His6-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and gel filtration Streptomyces lasalocidi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate methylation of geranyl diphosphate before the cyclization reaction Streptomyces lasalocidi S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.255 geranyl pyrophosphate methyltransferase
-
 Streptomyces lasalocidi
2.1.1.255 GPP methyltransferase
-
 Streptomyces lasalocidi
2.1.1.255 GPPMT
-
 Streptomyces lasalocidi

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.255 S-adenosyl-L-methionine
-
 Streptomyces lasalocidi