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Literature summary extracted from

  • Bischof, D.; Vilei, E.; Frey, J.
    Functional and antigenic properties of GlpO from Mycoplasma mycoides subsp. mycoides SC: Characterization of a flavin adenine dinucleotide-binding site deletion mutant (2009), Vet. Res., 40, 35.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.21 expressed in Escherichia coli BL21(DE3) cells Mycoplasma mycoides subsp. mycoides

Protein Variants

EC Number Protein Variants Comment Organism
1.1.3.21 additional information recombinant GlpO lacking the six amino acids Gly12-Gly13-Gly14-Ile15-Ile16-Gly17 (GlpODELTAFAD) is unable to bind FAD and is also devoid of glycerophosphate oxidase activity Mycoplasma mycoides subsp. mycoides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.21 45000
-
 two bands at 45000 and 90000 Da, SDS-PAGE Mycoplasma mycoides subsp. mycoides
1.1.3.21 90000
-
 two bands at 45000 and 90000 Da, SDS-PAGE Mycoplasma mycoides subsp. mycoides

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.21 Mycoplasma mycoides subsp. mycoides Q70GP9
-
-
1.1.3.21 Mycoplasma mycoides subsp. mycoides SC Afade Q70GP9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.21 Ni-NTA column chromatography Mycoplasma mycoides subsp. mycoides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.21 glycerol 3-phosphate + O2
-
 Mycoplasma mycoides subsp. mycoides dihydroxyacetone phosphate + H2O2
-
 ?
1.1.3.21 glycerol 3-phosphate + O2
-
 Mycoplasma mycoides subsp. mycoides SC Afade dihydroxyacetone phosphate + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.3.21 alpha-glycerol-3-phosphate oxidase
-
 Mycoplasma mycoides subsp. mycoides
1.1.3.21 GlpO
-
 Mycoplasma mycoides subsp. mycoides
1.1.3.21 L-alpha-glycerophosphate oxidase
-
 Mycoplasma mycoides subsp. mycoides

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.21 FAD
-
 Mycoplasma mycoides subsp. mycoides

General Information

EC Number General Information Comment Organism
1.1.3.21 physiological function GlpO is involved in production and translocation of toxic H2O2 into the host cell, causing inflammation and cell death Mycoplasma mycoides subsp. mycoides