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Literature summary extracted from

  • Gray, J.P.; Mishin, V.; Heck, D.E.; Laskin, D.L.; Laskin, J.D.
    Inhibition of NADPH cytochrome P450 reductase by the model sulfur mustard vesicant 2-chloroethyl ethyl sulfide is associated with increased production of reactive oxygen species (2010), Toxicol. Appl. Pharmacol., 247, 76-82.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.6.2.4 additional information in contrast to the inhibitory effects of 2-chloroethyl ethyl sulfide on redox cycling b cytochrome P450 reductase, spontaneous hydrogen peroxide production by the enzyme is stimulated by 2-chloroethyl ethyl sulfide. This response is concentration-dependent in the range of 0.1-10 mM, reaching a maximum at 0.3 mM Mus musculus
1.6.2.4 additional information in contrast to the inhibitory effects of 2-chloroethyl ethyl sulfide on redox cycling b cytochrome P450 reductase, spontaneous hydrogen peroxide production by the enzyme is stimulated by 2-chloroethyl ethyl sulfide. This response is concentration-dependent in the range of 0.1-10 mM, reaching a maximum at 0.3 mM Homo sapiens
1.6.2.4 additional information in contrast to the inhibitory effects of 2-chloroethyl ethyl sulfide on redox cycling b cytochrome P450 reductase, spontaneous hydrogen peroxide production by the enzyme is stimulated by 2-chloroethyl ethyl sulfide. This response is concentration-dependent in the range of 0.1-10 mM, reaching a maximum at 0.3 mM Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.2.4 2-chloroethyl ethyl sulfide potent inhibitor Homo sapiens
1.6.2.4 2-chloroethyl ethyl sulfide potent inhibitor Mus musculus
1.6.2.4 2-chloroethyl ethyl sulfide potent inhibitor Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.2.4 microsome
-
 Homo sapiens
-
-
1.6.2.4 microsome
-
 Rattus norvegicus
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.4 Homo sapiens
-
-
-
1.6.2.4 Mus musculus
-
-
-
1.6.2.4 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.2.4 liver
-
 Rattus norvegicus
-
1.6.2.4 MLE-15 cell type II lung epithelial cell Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.4 2 ferricytochrome c + NADPH
-
 Mus musculus 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 2 ferricytochrome c + NADPH
-
 Homo sapiens 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 2 ferricytochrome c + NADPH
-
 Rattus norvegicus 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 NADPH + H+ + oxidized 2,6-dichlorophenolindophenol
-
 Mus musculus NADP+ + reduced 2,6-dichlorophenolindophenol
-
 ?
1.6.2.4 NADPH + H+ + oxidized 2,6-dichlorophenolindophenol
-
 Homo sapiens NADP+ + reduced 2,6-dichlorophenolindophenol
-
 ?
1.6.2.4 NADPH + H+ + oxidized 2,6-dichlorophenolindophenol
-
 Rattus norvegicus NADP+ + reduced 2,6-dichlorophenolindophenol
-
 ?
1.6.2.4 NADPH + H+ + oxidized menadione
-
 Mus musculus NADP+ + reduced menadione
-
 ?
1.6.2.4 NADPH + H+ + oxidized menadione
-
 Homo sapiens NADP+ + reduced menadione
-
 ?
1.6.2.4 NADPH + H+ + oxidized menadione
-
 Rattus norvegicus NADP+ + reduced menadione
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.6.2.4 cytochrome P450 reductase
-
 Mus musculus
1.6.2.4 cytochrome P450 reductase
-
 Homo sapiens
1.6.2.4 cytochrome P450 reductase
-
 Rattus norvegicus
1.6.2.4 NADPH cytochrome P450 reductase
-
 Mus musculus
1.6.2.4 NADPH cytochrome P450 reductase
-
 Homo sapiens
1.6.2.4 NADPH cytochrome P450 reductase
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.4 FAD
-
 Mus musculus
1.6.2.4 FAD
-
 Homo sapiens
1.6.2.4 FAD
-
 Rattus norvegicus
1.6.2.4 FMN
-
 Mus musculus
1.6.2.4 FMN
-
 Homo sapiens
1.6.2.4 FMN
-
 Rattus norvegicus
1.6.2.4 NADPH
-
 Mus musculus
1.6.2.4 NADPH
-
 Homo sapiens
1.6.2.4 NADPH
-
 Rattus norvegicus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.6.2.4 0.38
-
 using ferricytochrome c as cosubstrate, in 10 mM phosphate buffer (pH 7.4), at 37°C Homo sapiens 2-chloroethyl ethyl sulfide
1.6.2.4 1.9
-
 using ferricytochrome c as cosubstrate, in 10 mM phosphate buffer (pH 7.4), at 37°C Mus musculus 2-chloroethyl ethyl sulfide
1.6.2.4 2.9
-
 in 10 mM phosphate buffer (pH 7.4), at 37°C Rattus norvegicus 2-chloroethyl ethyl sulfide
1.6.2.4 3.3
-
 using oxidized 2,6-dichlorophenolindophenol as cosubstrate, in 10 mM phosphate buffer (pH 7.4), at 37°C Mus musculus 2-chloroethyl ethyl sulfide
1.6.2.4 8.6
-
 using oxidized 2,6-dichlorophenolindophenol as cosubstrate, in 10 mM phosphate buffer (pH 7.4), at 37°C Homo sapiens 2-chloroethyl ethyl sulfide