Literature summary extracted from

Belingheri, L.; Cartayrade, A.; Pauly, G.; Gleizes, M.
Partial purification and properties of the sesquiterpene beta-selinene cyclase from Citrofortunella mitis fruits (1992), Plant Sci., 84, 129-136.

No PubMed abstract available

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
4.2.3.66	Mn2+	inhibitory above 0.2 mM, stimulation below	Citrus x microcarpa

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
4.2.3.66	0.045	-	(2E,6E)-farnesyl diphosphate	pH 7.6, 28°C	Citrus x microcarpa

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
4.2.3.66	Mg2+	maximum activity at 10 mM	Citrus x microcarpa
4.2.3.66	Mn2+	about 60% of the activity with Mg2+, inhibitory above 0.2 mM	Citrus x microcarpa
4.2.3.66	additional information	presence of divalent metal ion required, Mg2+ is preferred	Citrus x microcarpa

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
4.2.3.66	67000	-	gel filtration	Citrus x microcarpa

Organism

EC Number	Organism	UniProt	Comment	Textmining
4.2.3.66	Citrus x microcarpa	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
4.2.3.66	partial	Citrus x microcarpa

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
4.2.3.66	flavedo	-	Citrus x microcarpa	-
4.2.3.66	fruit	flavedo of immature fruit	Citrus x microcarpa	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
4.2.3.66	5180	-	pH 7.6, 28°C	Citrus x microcarpa

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4.2.3.66	(2E,6E)-farnesyl diphosphate	-	Citrus x microcarpa	beta-selinene + diphosphate	-	?

Subunits

EC Number	Subunits	Comment	Organism
4.2.3.66	monomer	1 * 67000, SDS-PAGE	Citrus x microcarpa

pI Value

EC Number	Organism	Comment	pI Value Maximum	pI Value
4.2.3.66	Citrus x microcarpa	isoelectric focusing	-	6

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Release 2023.1 (January 2023)